Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,517,508	G→T	V40V (GTG→GTT)	ortT →	orphan toxin OrtT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,517,508	0	G	T	100.0%	18.2 / NA	8	V40V (GTG→GTT)	ortT	orphan toxin OrtT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (4/4);  total (4/4)

GGTGAAAAAACTTGATGCACGTCAAAAAATGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCG  >  NC_000913/1517313‑1517672                                                                                                                                                                                                    |                                                                                                                                                                     ggTGAAAAAACTTGATGCACGTCAAAAAATGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTa                                                                                                                 <  1:3640/249‑1 (MQ=255)          aCTTGATGCACGTCAAAAAATGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTGTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCATGATAAGAAACGCATCCGTTTCGTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAg                                                                                                                                                  <  1:66260/207‑1 (MQ=255)                       cAAAAAATGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGc                                                                                         >  1:132749/1‑251 (MQ=255)                       cAAAAAATGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACATGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCa                                                                                                      >  1:23560/1‑238 (MQ=255)                                   cATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGCACATGTCTCTATATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTATTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGTTTGACGGCCCCACGCAGACt                                                                             >  1:129028/1‑251 (MQ=255)                                                                                                              gCGGTTAAGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCAGACGTTTCTTAAGCGCCTTTCGGGTTGGGGCAACATGGGCGATGTGTTTCCCGGTGGCGCTGATGTTTGCACTGTTTTAAATCTTAATCACTGGCTTGACGGCACAACGCTGTCTTGGATCAGTTGGATGAATCGGTAAATTGCACAATTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTGATcg  <  1:154811/251‑1 (MQ=255)                                                                                                                                                                  aGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGcac                                                                                       <  1:163501/114‑1 (MQ=255)                                                                                                                                                                                           cTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTAGCGCTGTTGTTTTCAATGTTTTAAATCTTACTCCCTGGCTTGACGGCACCCCGAAGACTtat                                                                          >  1:92010/1‑102 (MQ=255)                                                                                                                                                                                                    |                                                                                                                                                                     GGTGAAAAAACTTGATGCACGTCAAAAAATGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCG  >  NC_000913/1517313‑1517672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑