Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,353,758	T→A	D254V (GAT→GTT)	sapC ←	putrescine ABC exporter membrane protein SapC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,353,758	0	T	A	100.0%	16.8 / NA	9	D254V (GAT→GTT)	sapC	putrescine ABC exporter membrane protein SapC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (6/3);  total (6/3)

CCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGA‑TCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATA  >  NC_000913/1353582‑1353949                                                                                                                                                                                 |                                                                                                                                                                                                 ccGGTTGTAAATTAAATGGTCAGGTTACGAAGATCGAGTAATGGCAATATTCCCCCACCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATGGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAAGCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGA‑TCGAGAAAGCCCAGCGCGGCGATATCg                                                                                                                       <  1:116793/251‑1 (MQ=255)                                     aGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGA‑TCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTg                                                                                   <  1:465/251‑1 (MQ=255)                                              attattCCACCCCCGCAATAATCGCACGACGAACGCCAACGACAAGGAGGATAAACACCAAGACGCCAATCACAATTGCCGAACCTAGCAGCATGACCGTCCACGGAGCCGCATAAATCAGTTCCGGCACAAAACCAAGCATCGCTCCCCATGCAGGCGAGCGGCGTGGTGCGACG‑GACTCGAGAAAGCCAAAAGCGGCGCAATCGAGCACGGCCATAGACAGAGCGCGAATGATCTCGGTCACCAGACcg                                                                          >  1:164247/1‑251 (MQ=255)                                                                        cgacgaACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAACTCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGA‑TCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGAtc                                                                                         >  1:73544/1‑210 (MQ=255)                                                                                       ccAAGGAGNTNNNCNNACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCNACGGGGCGACATAAANCANTTCCNGCGNAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGA‑TCGAGAAAGCCCAGCgcggc                                                                                                                              >  2:120902/1‑158 (MQ=255)                                                                                       ccAAGGAGGTTAACCAACAACACGCTAATCATAAGTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGA‑TCGAGAAAGCCCAGCgcggc                                                                                                                              <  1:120902/158‑1 (MQ=255)                                                                                                          ncacGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGA‑TCGAGAAAGCCCAGCGCGGCGATATCGAGAATCGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATgcgc                      >  1:35017/2‑243 (MQ=255)                                                                                                                       aaTTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGA‑TCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATa  >  1:86475/1‑250 (MQ=255)                                                                                                                                                        ggCGACATAAATCAGTTCCAGCGCAACACCGCGAATCGCTCCCCATTCAGGc                                                                                                                                                                        >  1:54070/1‑52 (MQ=255)                                                                                                                                                                                 |                                                                                                                                                                                                 CCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGA‑TCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATA  >  NC_000913/1353582‑1353949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑