Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	948,464	G→A	K412K (AAG→AAA)	ycaM →	putative transporter YcaM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	948,464	0	G	A	88.9%	17.0 / ‑6.0	9	K412K (AAG→AAA)	ycaM	putative transporter YcaM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (5/3);  minor base T (0/1);  total (5/4)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGATTCTGATTATGCTGCCGACTCTCGGCATTGGTGATATGAACAATCTCTATAAATGGCTGTTGAACCTTAATTCGGTAGTGATGCCGCTGCGTTATTTATGGGTATTTGTTGCATTTATTGCAGTCGTTCGCTTGGCGCAGAAATATAAACCAGAATATGTCTTTATTCGTAATAAGCCTCTGGCAATGACCGTCGGGATTTGGTGTTTTGCCTTTACCGCCTTTGCCTGTTTGACGGGGATCTTCCCGAAAATGGAAGCCTTCACTGCAGAGTGGACCTTCCAGTTGGCGCTGAATGTTGCAACGCCGTTTGTGCTGGTAGGATTAGGACTGATATTCCCGCTGCTGGCGCGTAAAGCGAATAGTAAATAATTATTGTGGTCGGTGACGCTGACTCTG  >  NC_000913/948284‑948686                                                                                                                                                                                     |                                                                                                                                                                                                                               ggCGATTCTGATTATGCTGCCGACTCTCGGCATTGGTGATATGAACAATCTCTATAAATGGCTGTTGAACCTTAATTCGGTAGTGATGCCGCTGCGTTATTTATGGGTATTTGTTGCATTTATTGCAGTCGTTCGCTTGGCGCAGAAATATAAACCAGAATATGTCTTTATTCGTAATAATCCTCTGGCAATGTCCGTCGGGATTTGGTGTTTTGCCTTTACCGCCTTGGCCTGTTTGACGGGGATCTTc                                                                                                                                                           <  1:35581/250‑1 (MQ=255)                                                             gCTGTTGAACCTTAATTCGGTAGTGATGCCGCTGCGTTATTTATGGGTATTTGTTGCATTTATTGCAGTCGTTCGCTTGGCGCAGAAATATAAACCAGAATATGTCTTTATTCGTAATAAACCTCTGGCAATGACCGTCGGGATTTGGCGTTTTGCCTTTACCGCCTTTGCCTGTTTGACGGGGATCTTCCCGAAAATGGAAGCCTTCACTGCAGAGTGGACCTTCCAGTTGGCGCTGAATGTTGCAACGc                                                                                              >  1:168279/1‑251 (MQ=255)                                                             gCTGTTGAACCTTAATTCGGTAGTGATGCCGCTGCGTAATTTATGGGTATTTGTTGAATTTATTGCAGTAGTTAGCTTGGCGCAGAAATATAAACCAGAATATGTCTTTATTCGTAATAAACCACTGGCAATGACCGTCGGGATTTGGCGTTTTGCCTTTACCGCCTTTGCCTGTTTGACGGGGATCTTCCCGAAAATGGAAGCCTTCACTGCCGAGTGGACCTTCCAGTTGGCTCTGAATGTTGCAACGc                                                                                              >  1:168281/1‑251 (MQ=255)                                                                gTTGAACCTTAATTCGGTAGTGATGCCGCTGCGTTATTTATGGGTATTTGTTGCATTTATTGCAGTCGTTCGCTTGGCGCAGAAATATAAACCAGAATATGTCTTTATTCGTAATAAACCTCTGGCAATGACCGTCGGGATTTGGTGTTTTGCCTTTACCGCCTTTGCCTGTTTGACGGGGATCTTCCCGAAAATGGAAGCCTTCACTGCAGAGTGGACCTTCCAGTTGGCGCTGAATGTTGCAACGCCGt                                                                                           >  1:61521/1‑251 (MQ=255)                                                                                   gTGATGCCGCTGCGTTATTTATGGGTATTTGTTGCATTTATTGCAGTCGTTCGCTTGGCGCAGAAATATAAACCAGAATATGTCTTTATTCGTAATAAACCTCTGGCAATGACCGTCGGGATTTGGTGTTTTGCCTTTACCGCCTTTGCCTGTTTGACGGGGATCTTCCCGAAAATGGAAGCCTTCACTGCAGAGTGGACCGTCCAGTTGGCGCTGAATGTTGCAACGCCGTTTGTGCTGGTAGGATTAgg                                                                        <  1:111521/251‑1 (MQ=255)                                                                                                   atttatGGGTATTTGTTGCATTTATTGCAGTCGTTCGCTTGGCGCAGAAATATAAACCAGAATATGTCTTTATTCGTAATAAACCTCTGGCAATGACCGTCGGGATTTGGTGTTTTGCCTTTACCGCCTTTGCCTGGTTGACGGGGATCTTCCCGAAAATGGAAGCCTTCACTGCAGAGTGGACCTTCCAGTTGGCGCTGAATGTTGCAACGCCGTTTGTGCTGGTAGGAATAGGACTGATATTCCCgctg                                                        <  1:39468/251‑1 (MQ=255)                                                                                                                                          tGGCGCAGAAATATAAACCAGAATATGTCTTTATTCGTAATAAACCTCTGGCAATGACCGTCGGGATTTGGTGTTTTGCCTTTACCGCCTTTGCCTGTTTGACGGGGATCTTCCCGAAAATGGAAGCCTTCACTGCAGAGTGGACCTTc                                                                                                                       <  1:3566/149‑1 (MQ=255)                                                                                                                                                        aaaCCAGAATATGTCTTTATTCGTAATAAACCTCTGGCAATGACCGTCGGGATTTGGTGTTTTGCCTTTACCGCCTTTGCCTGTTTGACGGGGATCTTCCCGAAAATGGAAGCCTTCACTGCAGAGTGGACCTTCCAGTTGGCGCTGAATGTTGCAACGCCGTTTGTGCTGGTAGGATTAGGACTGATATTCCCGCTGCTGGCGCGTAAAGCGAATAGTAAATAATTATTGTGGTCGGTGACGCTGActct   >  1:141891/1‑251 (MQ=255)                                                                                                                                                         aaCCAGAATATGTCTTTATTCGTAATAAACCTCTGGCAATGACCGTCGGGATTTGGTGTTTTGCCTTTACCGCCTTTGCCTGTTTGACGGGGATCTTCCCGAAAATGGAAGCCTTCACTGCAGAGTGGACCTTCCAGTTGGCGCTGAATGTTGCAACGCCGTTTGTGCTGGTAGGATTAGGACTGATATTCCCGCTGCTGGCGCGTAAAGCGAATAGTAAATAATTATTGTGGTCGGTGACGCTGACTCTg  >  1:85165/1‑251 (MQ=255)                                                                                                                                                                                     |                                                                                                                                                                                                                               GGCGATTCTGATTATGCTGCCGACTCTCGGCATTGGTGATATGAACAATCTCTATAAATGGCTGTTGAACCTTAATTCGGTAGTGATGCCGCTGCGTTATTTATGGGTATTTGTTGCATTTATTGCAGTCGTTCGCTTGGCGCAGAAATATAAACCAGAATATGTCTTTATTCGTAATAAGCCTCTGGCAATGACCGTCGGGATTTGGTGTTTTGCCTTTACCGCCTTTGCCTGTTTGACGGGGATCTTCCCGAAAATGGAAGCCTTCACTGCAGAGTGGACCTTCCAGTTGGCGCTGAATGTTGCAACGCCGTTTGTGCTGGTAGGATTAGGACTGATATTCCCGCTGCTGGCGCGTAAAGCGAATAGTAAATAATTATTGTGGTCGGTGACGCTGACTCTG  >  NC_000913/948284‑948686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑