Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	895,783	G→A	E265K (GAA→AAA)	potG →	putrescine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	895,783	0	G	A	100.0%	24.4 / NA	10	E265K (GAA→AAA)	potG	putrescine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/7);  total (3/7)

CTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCTTGATTCGCCGGGGCTGGTGCATCCACTGAAAGTCGACGCGGATGCCTCGGTGGTCGATAACGTGCCGGTACATGTGGCGCTGCGCCCGGAAAAAATCATGCTTTGCGAAGAGCCGCCCGCCAATGGTTGTAACTTCGCGGTGGGGGAGGTGATACACATTGCCTATCTCGGCGATCTTTCGGTGTATCACGTTCGTCTGAAAAGTGGGCAGATGATTAGCG  >  NC_000913/895576‑896020                                                                                                                                                                                                                |                                                                                                                                                                                                                                              cTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGAAAGATGGCCTGGTGCTTGATTCg                                                                                                                                                                                                                        <  1:56430/231‑1 (MQ=255)      gcgcGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGAAAGATGGCCTGGTGCTTGATTCGCCGGGGCTGGTGCATCCACTGAAAg                                                                                                                                                                                               <  1:58750/251‑1 (MQ=255)                gtgACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGTCATTATGAATCGTGGGACATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTCTTGGCTCGGAAAACGTCTTTGAAGGAGTACCCAAAAAGCGTCAGAAAGATGGCCTGGCGCTTGCTTTGCCGGGGCTGGGGCCTCCCCTGAAAGTCGACGCgg                                                                                                                                                                                      >  1:152852/1‑250 (MQ=255)                                       cGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTAGAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAGGGCGTACTCAAAGAGCGTCAGAAAGATGGCCTGGTGCGGGAGTCGCCGGGGCTGGTGCATCCACTGAAAGTCGACGCGGATGCCTCGGTGGTCGATAACGTGc                                                                                                                                                              <  1:114629/251‑1 (MQ=255)                                                                                                       naGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGAAAGATGGCCTGGTGCTTGATTCGCCGGGGCTGGTGCATCCACTGAAAGTCGACGCGGATGCCTCGGTGGTCGATAACGTGCCGGTACATGTGGCGCTGCGCCCGGAAAAAATCATGCTTTGCGAAGAGCCGCCCGCCAATGGTTg                                                                                              >  1:43422/2‑251 (MQ=255)                                                                                                                  aCCGGAAGAGACCTACGAGCATCCGACTACCCGCTATTGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGGACTCAAAGAGCGTCAGAAAGATGGCCTGGTGCTGGATTCGCCGGGGATGGTGCATCCACTGAAAGTCGACGCGGAGGCCTCGGTGGTCTATACCGTGCAGGGCCATGGGGTGCTCCGCCCGGAAAAAATCAGGCTTTGCGAAGAGCCGCCCGCCATTGGTTGTAACTTCGCgg                                                                                   <  1:127850/251‑1 (MQ=255)                                                                                                                               tACGAGCATCCTACTACCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGAAAGATGGCCTGGTTCTTGTTTCGCGGGGGCTGGGTCATCCGCTGAAAGTCTACGCGGAGGCCTCGGTGGTGGATAACGTGCCGGTCCATGTGGCGCGGCGCCGGTAAAAAATCAGGCTTTGCGAAGAGCCGCCCGCCAATGGTTGTAACTTCGCGGTGGGGGa                                                                            <  1:78471/245‑1 (MQ=255)                                                                                                                                              aCCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGAAAGATGGCCTGGTGCTTGATTCGCCGGGGCTGGTGCATCCACTGAAAGTCGACGGGGATGCCTCGGTGGTCGATAACGTGCCGGTACAtgtn                                                                                                                                                   <  1:19322/159‑2 (MQ=255)                                                                                                                                                                   aTTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGAAAGATGGCCTGGTGCTTGATTCGCCGGGGCTGGTGCATCCACTGAAAGTCGACGCGGATGCCTCGGTGGTCGATAACGTGCCGGTACATGTGGCGCTGCGCCCGGAAAAAATCATGCTTTGCGAATAGCCGCCCGCCAATGGTTGTAACTTCGCGGTGGGGGAGGTGATACACATTGCCTATCTCGGCGATCTTTCGGTGTATc                                   <  1:148859/250‑1 (MQ=255)                                                                                                                                                                                                   cAAAGAGCGTCAGAAAGATGGCCTGGTGCTTGATTCGCCGGGGCTGGTGCATCCACTGAAAGTCGACGCGGATGCCTCGGTGGTCGATAACGTGCCGGTACATGTGGCGCTGCGCCCGGAAAAAATCATGCTTTGCGAAGAGCCGCCCGCCAATGGTTGTAACTTCGCGGTGGGGGAGGTGATACACATTGCCTATCTCGGCGATCTTTCGGTGTATCACGTTCGTCTGAAAAGTGGGCAGATGATTAgcg  >  1:143346/1‑251 (MQ=255)                                                                                                                                                                                                                |                                                                                                                                                                                                                                              CTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCTTGATTCGCCGGGGCTGGTGCATCCACTGAAAGTCGACGCGGATGCCTCGGTGGTCGATAACGTGCCGGTACATGTGGCGCTGCGCCCGGAAAAAATCATGCTTTGCGAAGAGCCGCCCGCCAATGGTTGTAACTTCGCGGTGGGGGAGGTGATACACATTGCCTATCTCGGCGATCTTTCGGTGTATCACGTTCGTCTGAAAAGTGGGCAGATGATTAGCG  >  NC_000913/895576‑896020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑