Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	823,053	G→A	Y229Y (TAC→TAT)	clsB ←	cardiolipin synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	823,053	0	G	A	100.0%	41.4 / NA	16	Y229Y (TAC→TAT)	clsB	cardiolipin synthase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/9);  total (7/9)

CTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTCAAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAGTAGGCGTTGGCGATAATCACTTCCCGCCGCGCCTGAGTGAGCATTTTCAAATAATGGCGTTCAATATCATCGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGC  >  NC_000913/822833‑823281                                                                                                                                                                                                                             |                                                                                                                                                                                                                                     cTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTCAAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAATAggn                                                                                                                                                                                                                                 <  1:42374/226‑2 (MQ=255)             gATCGCCCATCATTGCCACTTTCCCGGGGAGCGGCCGGCGCCGGTACTCAAAACCCTGAACGCCGCTTTTAACCAGATAGTTAAACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAATAGGCGTTGGCGATAATCCCTTCCCGCCGCGCCtgagtgag                                                                                                                                                                                             <  1:693/250‑1 (MQ=255)                                                             cAAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTT‑CGCAAGGCGTGTAAAAATCGCTAGCCGGGGAAGAAATAGGCGTTGGCGATAATCACTTCCCGCCGCGCCTGAGTGAGCATTTTCAAATAATGGCGtt                                                                                                                                                                         >  1:79947/1‑221 (MQ=255)                                                              aaaaaCCTGCACGCCGCCTTTAACCANATAGTTNNACNGCAAGCGCGCACCGACTCTNACNNNCNNNANCTCAGNTTNNCCCTGAATGNTCAGTTTGATCCGCCCCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATNGATAGCCGGNNAAGAAATAgg                                                                                                                                                                                                                                  >  2:40531/1‑164 (MQ=255)                                                              aaaaaCCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGGTTGATCCGCACCCCGCGGCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAATAgg                                                                                                                                                                                                                                  <  1:40531/164‑1 (MQ=255)                                                                 aaCCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACACTCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCCAGGCGTGTAAAAATCGATAGCCGGGGACGAAATAGGCGTTGGCGATAATCACTTCCCGCCGCGCCtgagtgag                                                                                                                                                                                             >  1:62958/1‑198 (MQ=255)                                                                         cgccgcCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAAGCGATAGCCGGGGAAGAAATAGGCGTTGGCGATAATCACTTCCCGCCGCGCCTGAGTGAGCATTTTCAAATAATGGCGTTCAATATCATCGCGATGTTCTTCGTTATCGCGCCAGACCAgn                                                                                                                                <  1:34513/251‑2 (MQ=255)                                                                                                  cAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGCTCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAATAGGCGTTCGCGCTAATCACTTCCCGCCGCGCCTGCGTGAGCATTTTCAGATAATGGCGTTCAATATCATcgcg                                                                                                                                                            >  1:86602/1‑198 (MQ=255)                                                                                                                     ctGACAATCGGCATATCCGGTTAGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAATAGGCGTTGGCGATAATCACTTCccgc                                                                                                                                                                                                           >  1:56723/1‑132 (MQ=255)                                                                                                                                   atCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAATAGGCGTTGGCGATAATCACTTCCCGCCGCGCCTGAGTGAGCATTTTCAAATAAAGGCGTTCAATATCATCGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGccac                                                                       <  1:11830/250‑1 (MQ=255)                                                                                                                                                            ttGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAATAGGCGTTGGCGATAATCCCTTCCCGCCGCGCCTGAGTGAGCATTTTCAAATAATGGCGTTCAATATCATCGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCcag                                              <  1:53370/250‑1 (MQ=255)                                                                                                                                                                cccGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAATAGGCGTTGGCGATAATCACTTCCCGCCGCGCCTGAGTGAGCATTTTCAAATAATGGCGTTCAATATCATCGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCcaggcagn                                         <  1:103650/250‑2 (MQ=255)                                                                                                                                                                               gTGCCGATTTACGCAAGGCGAGTAAAAATCGATAGCCGGGGAAGAAATAGGCGGTGGGGATAGTAAATTCCCGCCGAGCCTGAGTGAGCATTTTCAAATAATGGCGTTCAATATCATCGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTTTCTTACGCTTTGTGATGACGTCGCCCCCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCCGCTCaaa                           >  1:144788/1‑250 (MQ=255)                                                                                                                                                                                  ccGCTTTACGCAAGGCGTGTAAAAATCGATAGCNNGNNNANAAATAGGc                                                                                                                                                                                                                                 <  2:123869/49‑1 (MQ=255)                                                                                                                                                                                  ccGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGGAGAAATAgga                                                                                                                                                                                                                                 >  1:123869/1‑48 (MQ=255)                                                                                                                                                                                                       aaaaTCGATAGCCGGGGAAGAAATAGGCGTGGGCGATAATCACTTCCCGCCGCGCCTGAGTGAGCATTTTCAAATAATGGCGTTCAATATCATCGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCGGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCggn  <  1:20803/251‑2 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                     CTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTCAAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAGTAGGCGTTGGCGATAATCACTTCCCGCCGCGCCTGAGTGAGCATTTTCAAATAATGGCGTTCAATATCATCGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGC  >  NC_000913/822833‑823281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑