Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	810,780	G→A	A134T (GCC→ACC)	bioF →	8‑amino‑7‑oxononanoate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	810,780	0	G	A	100.0%	24.8 / NA	11	A134T (GCC→ACC)	bioF	8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/6);  total (5/6)

GCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAAAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGATTGCTTGCTTCCCCCTGTCCGGGGCAGCAAATGGTGGTGACAGAAGGCGTGTTCAGCATGGACGGCGATAGTGCGCCACTGGCGGAAATCCAGCAGGTAACGCAACAGCACAATGGCTGGTTGATGGTCGATGATGCCCACGGCACGGGCGTTATCGGG  >  NC_000913/810619‑811019                                                                                                                                                                  |                                                                                                                                                                                                                                                gCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGATAATCAGGCAGTTATTGCCGCGATGATGGCGAAAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATACCTCATTGCTGGAAGCTGCCAGTTTAAGCTCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGGCTCTCATTTTGCGCGTTtgcttgct                                                                                                                                                        <  1:55668/251‑1 (MQ=255)                          aCTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAAAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATACCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCTCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGATTGCTTGCTTCCCCCTGTCCGGGGCAGCAAAtgg                                                                                                                               <  1:117175/251‑1 (MQ=255)                            tGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTTGCGGGCACTGCTGTTTGTCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGAGATGATGGCGAAAGAGGACCGTATTACTGCCGACCGGCTTAGCCATACCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTTCTCATAACGATGTCACTCATTTGGCGCGATTGCTTGCTTCCCCCTGTCCGGGGCAGAAAAtggtg                                                                                                                             >  1:156497/1‑251 (MQ=255)                                                 ggcttggctATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAAAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATACCTCATTGCTGGAAGCTGcc                                                                                                                                                                                                                             <  1:6310/134‑1 (MQ=255)                                                                         ggTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAAAGAGGACCGTATTGCTGCCGACCGGGTTAGCCGTACCTCAGTGCTGGAAGCGGGCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATTTCACTCAATTGGCGCGATTGCTGGCTTCCCCCTGTCCgggg                                                                                                                                         <  1:154524/193‑1 (MQ=255)                                                                                             gcTAATCAGGCAGTTATTGCCGCGATGATGGCGAAAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATACCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGATTGCTTGCTTccccc                                                                                                                                                  <  1:12370/165‑1 (MQ=255)                                                                                                    aGGCAGTTATTGCCGCGATGATGGCGAAAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATACCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGATTGCTTGCTTCCCCCTGTCCGGGGCAGCAAATGGTGGTGACAGAAGGCGTGTTCAGCATGGACGGCGATAGTGCGCCACTGGCGGAAATCCAGCAGGTAACGCAACAg                                                     <  1:146158/251‑1 (MQ=255)                                                                                                     ggCAGTTATTGCCGCGATGATGGCGAAAGAGGACCGTATTGGTGCCGACCGGCTTAGCCATACCTACTTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCAATCATTTGGCGCGATTGCTTGCTTCCCCCTGTACGGGGCAGCAAATGGTGGTGACAGAAGGCGTGTTCAGCATGGACGGCGATAATGCGCCACTGGCGGAAATCCAGCAGGTAACGCAACAGc                                                    >  1:83885/1‑251 (MQ=255)                                                                                                                             gAAAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATACCTCATTGCTGGAAGCTACCCGTTTAAGCCCGTCGCAGATTCGCCGTTTAGCACATACCGATGTCACTCATTTGGCGAGATTGCTTGCTTCCACCTGTCCgggg                                                                                                                                         >  1:122522/1‑142 (MQ=255)                                                                                                                                                       ggCTTAGCCATACCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGATTGCTTGCTTCCCCCTGTCCGGGGCAGCAAATGGTGGTGACAGAAGGCGTGTTCAGTATGGACGGCGATAGTGCGCCACTGGCGGAAATCCAGCAGGTAACGCAACAGCACAATGGCTGGTTGATGGTCGATGATGCCCACGGCACGGGCGTTATCggg  >  1:173385/1‑251 (MQ=255)                                                                                                                                                             gCCATACCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGATTGCTTGCTTCCCCCTGTCCGGGGCAGCAAATGGTGGTGACAGAAGGCGTGTTCAGCATGGACGGCGATAGTGCGCCACTGGCGGAAATCCAGCAGGTAACGCAACAGCACAATGGCt                                           >  1:95432/1‑204 (MQ=255)                                                                                                                                                                  |                                                                                                                                                                                                                                                GCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAAAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGATTGCTTGCTTCCCCCTGTCCGGGGCAGCAAATGGTGGTGACAGAAGGCGTGTTCAGCATGGACGGCGATAGTGCGCCACTGGCGGAAATCCAGCAGGTAACGCAACAGCACAATGGCTGGTTGATGGTCGATGATGCCCACGGCACGGGCGTTATCGGG  >  NC_000913/810619‑811019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑