Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	805,726	G→A	G742S (GGT→AGT)	ybhJ →	putative hydratase YbhJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	805,726	0	G	A	100.0%	13.0 / NA	6	G742S (GGT→AGT)	ybhJ	putative hydratase YbhJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/3);  total (3/3)

GCTGCAAATGGCGGAAGTACCAACCTTTGAAGTGGGGGATTACATTTACATCCCTGGCATTAAAGCGGCGCTGGATAATCCGGGTACGACGTTTAAAGGTTATGTGATCCATGAAGATGCGCCGGTAACGGAAATTACGCTCTATATGGAAAGTCTGACTGCTGAAGAGCGCGAGATTATCAAGGCGGGTAGTTTGATTAACTTCAATAAAAACCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGC  >  NC_000913/805539‑805971                                                                                                                                                                                            |                                                                                                                                                                                                                                                      gCTGCAAATGGCGGAAGTACCAACCTTTGAAGTGGGGGATTACATTTACATCCCTGGAATTCAAGCGGCGATGGATCATCAGGGCACGAAGTTTCAAGGTTATGTGATCCATGAAGATGCGCCGGTAACGGAAATTACGCTCTATATGGCCAGTCTGACTGCTGAAGAGCGCGAGATTATCAAGGCGAGTAGTTTGATTAACTTCAATAAAAACCGTCAGATGTAAAAAGCGCAATGAGACTGTAGGTCGc                                                                                                                                                                                        >  1:162341/1‑251 (MQ=255)                                  gggggATTACATTTACATCCCTGGCATTAAAGCGGCGCTGGATAATCCGGGTACGACGTTTAAAGGTTATGTGATCCATGAAGATGCGCCGGTAACGGAAATTACGCTCTATATGGAAAGTCTGACTGCTGAAGAGCGCGAGATTATCAAGGCGAGTAGTTTGATTAACTTCAATAAAAACCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTGGCATTCGTCACTTATTGTCGGATGCGATGTTTgcg                                                                                                                                                       <  1:138294/251‑1 (MQ=255)                                   ggggATTACATTTACATCCCTGGCATTAAAGCGGCGCTGGATAATCCGGGTACGACGTTTAAAGGTTATGTGATCCATGAAGATGCGCCGGTAACGGAAATTACGCTCTATATGGAAAGTCTGACTGCTGAAGAGCGCGAGATTATCAAGGCGAGTAGTTTGATTAACTTCAATAAAAACCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTgcgc                                                                                                                                                      >  1:5727/1‑251 (MQ=255)                                                        ggCATTAAAGCGGCGCTGGATAATCCGGGTACGACGTTTAAAGGTTATGTGATCCATGAAGATGCGCCGGTAACGGAAATTACGCTCTATATGGAAAGTCTGACTGCTGAAGAGCGCGAGATTATCAAGGCGAGTAGTTTGATTAACTGCAATAAAAACCGGCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACGAATc                                                                                                                                 <  1:87175/251‑1 (MQ=255)                                                                                                                                                                    gAAGAGCGCGAGATTATCAAGGCGAGTAGTTTGATTAACTTCAATAAAAACCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATc                                                                                                                                                   >  1:92364/1‑125 (MQ=255)                                                                                                                                                                                       aGGCGAGTAGTTTGATTAACTTCAATAAAAACCGTCAGATGTAAAAATCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACTAATCGCATCTAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGAGGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTAcgc  <  1:130139/251‑1 (MQ=255)                                                                                                                                                                                            |                                                                                                                                                                                                                                                      GCTGCAAATGGCGGAAGTACCAACCTTTGAAGTGGGGGATTACATTTACATCCCTGGCATTAAAGCGGCGCTGGATAATCCGGGTACGACGTTTAAAGGTTATGTGATCCATGAAGATGCGCCGGTAACGGAAATTACGCTCTATATGGAAAGTCTGACTGCTGAAGAGCGCGAGATTATCAAGGCGGGTAGTTTGATTAACTTCAATAAAAACCGTCAGATGTAAAAAGCGCCATGTGAATGTAGGTCGCATTCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGC  >  NC_000913/805539‑805971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑