Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	788,566	G→A	T91I (ACC→ATC)	galM ←	galactose‑1‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	788,566	0	G	A	100.0%	22.5 / NA	10	T91I (ACC→ATC)	galM	galactose‑1‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

CGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGC  >  NC_000913/788354‑788778                                                                                                                                                                                                                     |                                                                                                                                                                                                                     cgcgATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGCTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGATTTCACCGTCAAAGGTATAACGGCTATTGGCGATACgg                                                                                                                                                                                >  1:174314/1‑251 (MQ=255)         gtaatgtatattcgtttgtcgtcgGTCAGACGATATTGCAGTGTCGCGCCGAGATTACCCGCGAAGCCCTGATCGCCATCATCTGAACCCAGGGCAAACAGCACCTGACCATCGTTCTGTTTCACGGTTGGCCAGCGACGTTTGGCGAGCCCTTCCTGCCCGCCGTGCTTCTGGTTTACGCCCTGTCTTTGCGAAGTCTTCACGATTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATa                                                                                                                                                                        <  1:87813/233‑1 (MQ=255)                                              gCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACAACCATCTGAAATCCGCGCAAAAAGCACCTGAAGCTCATTCTGGGTCACCCTCTGCCAGCGACGTTTGTCGAACCCTTACGGCCCGCCCTGCAGCTCGTTAACGCCCTGTATTGGCGAAAGCGTCAAGATTTCACCGTCACAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGcctgatcc                                                                                                                                   >  1:175319/1‑251 (MQ=255)                                                                                                  cAGGGCCAACAGCACCTGACGATAGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGATTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGagc                                                                                                     >  1:48422/1‑229 (MQ=255)                                                                                                                                      cagagtgtgctcttccgatctgAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGATTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCan                                           <  1:106485/230‑2 (MQ=255)                                                                                                                                                   cGTTTGTCGAACCCTTCCGGNCCGCCGGGCNNCTNGTTAANGNNNTNNNTTNGCGAAAGCGTCACGATTTCNCCNNCAAAGGNATAACGGCTATTGGCGATACGGTTgg                                                                                                                                                                            <  2:40145/109‑1 (MQ=255)                                                                                                                                                   cGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGATTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTgg                                                                                                                                                                            >  1:40145/1‑109 (MQ=255)                                                                                                                                                           gAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGATTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGNcctnnnnntgnn                                                                                                                               >  2:106485/1‑144 (MQ=255)                                                                                                                                                                       cccGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGATTTCACCGTCAAAGGAATAACGGCTATTGGCGATACGGTTGNNATACCGNnc                                                                                                                                                                  >  2:2619/1‑99 (MQ=255)                                                                                                                                                                               gCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGATTTCACCGTCAAAGGTATAACGGCTATTGGCGATTCGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGc  <  1:2619/251‑1 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                     CGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGC  >  NC_000913/788354‑788778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑