Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 776,964 G→A R208K (AGA→AAA)  tolA → Tol‑Pal system protein TolA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913776,9640GA100.0% 16.7 / NA 9R208K (AGA→AAA) tolATol‑Pal system protein TolA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/3);  total (6/3)

AGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCAGATGCTAAAGCTGCGGAAGAAGCAGCGAAGAAAGCGGCTGCAGACGCAAAGAAAAAAGCAGAAGCAGAAGCCGCCAAAGCCGCAGCCGAAGCGCAGAAAAAAGCCGAGGCAGCCGCTGCGGCACTGAAGAAGAAAGCGGAAGCGGCAGAAGCAGCTGCAGCTGAAGCAAGAAAGAAAGCGGCAACTGAAGCTGCTGAAAAAGCCAAAGCAGAAGCTGAGAAGAAAGCGGCTGCTGAAAAGGCTGCAGCTGATAAGAAAGCGGCAGCAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCG  >  NC_000913/776740‑777197
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         
agcTGCAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGACGGCCGAAGCAGATGCTAAAGCTGCGGAAGAACCAGCGAAGAAACCGGCTGCAGACGCACAGACAAAAGCAGAAGCAGAAGCCGCCAAAGCCGCAGCCGAAGCGCAGAAAAAAGCCGAGGCAGCCGCTGCGGCACTGAAGAAGAAAGCGGAAGCGGCAGAAGCAGCGGCAGCTGAAGCAAAAAAGAAAGCAGCAACTGAAGctgctg                                                                                                                                                                                                                 >  1:997/1‑251 (MQ=255)
                                               ccGAAGCAGATGCTAAAGCTGCGGAAGAAGCAGCGAAGAAAGCGGCTGCAGACGCAAAGAAAACAGCAGAAGCAGAAGCCGCCAAAGCCGCCGCCGAAGCGCAGAAAAAAGCCGAGGCAGCCGCTGCGGCACTGAAGAAGAAAGCGGAAGCGGCAGAAGCAGCTGCAGCTGAAGCAAAAAAGAAAGCGGCAACTGAAGCTGCTGAAAAAGCCAAAGCAGCAGCTGAGAAGAAAGCGGCTGCTGAAAAGGCt                                                                                                                                                                  >  1:113422/1‑251 (MQ=255)
                                                                                cGAAGAAAGCGGCTGCAGACGCAAAGAAAAAAGCAGAAGCAGAAGCCGCCAAAGCCGCAGCCGAAGCGCAGAAAAAAGCCGAGGCAGCCGCTGCGGCACTGAAGAAGAAAGCGGAAGCGGCAGAAGCAGCTGCAGCTGAAGCAAAAAAGAAAGCGGCAACTGAAGCTGCTGAAAAAGCCAAAGCAGAAGCTGAGAAGAAAGCGGCTGCTGAAAAGGCTGCAGCTGATAAGAAAGCGGCAGCAGAGAAAGCt                                                                                                                                 >  1:118920/1‑251 (MQ=255)
                                                                                                                             ccgccAAAGCCGCAGCCGAAGCGCAGAAAAAAGCCGAGGCAGCCGCTGCGGCACTGAAGAAGAAAGCGGAAGCGGCAGAAGCAGCTGCAGCTGCAGCAAAAAAGAAAGCGGCAACTGAAGCTGCTGAAAAAGc                                                                                                                                                                                                          >  1:176085/1‑133 (MQ=255)
                                                                                                                                                      aaaaaaaGCCGAGGCAGCCGCTGCGGCACTGAAGAAGAAAGGGGAAGCGGCAGAAGCAGCGGCAGCTGAAGCAAAAAAGAAAGCGGCAACTGAATCGTCTGAAAAAGCAAAAGCAGAAGCTGAGAATAAAGCTGCTGCTGAAAAGGCTGCAGCTAATAAGAAAGCGGCATCAGAGAAAGCGTCAGCCGATAAAAAAGCAGCATAAAAAGCGGCTGCTGAAAAGGcagcag                                                                                <  1:140051/229‑1 (MQ=255)
                                                                                                                                                                                    naagaagnnAGCGGAAGCGGCAGAAGGAGCTGCAGCTGAATCAAAAAAGAAAGCGGCAACTGAAGCTGCGGAAAAAGCCAAAGCAGAAGCTgagatg                                                                                                                                                                                       <  2:997/96‑3 (MQ=255)
                                                                                                                                                                                      anaanaaAGCNGAAGNGGCAGAAGCAGCTGCNGCTGAAGCTAAAAAGAAAGNNGNATCTTNAgctg                                                                                                                                                                                                                    <  2:70589/66‑1 (MQ=255)
                                                                                                                                                                                             agcggaagcggCAGAAGCAGCTGCAGCTGAAGCAAAAAAGAAAGCGGCAACTGAAGCTGCTGAAAAAGCCAAAGCAGAAGCTGAGAAGAAAGCGGCTGCTGAAAAGGCTGCAGCTGATAAGAAAGCGGCAGCAGAGAAAGCTGCAGCCGAc                                                                                                                        >  1:11156/1‑151 (MQ=255)
                                                                                                                                                                                                               agctgcagctgcAGCAAAAAAGAAAGCGGCAACTGAAGCTGCTGAAAAAGCCAAAGCAGAAGCTGAGAAGAAAGCGGCTGCTGAAAAGGCTGCAGCTGATAAGAAAGCGGCAGCAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCg  >  1:12163/1‑251 (MQ=255)
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         
AGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCAGATGCTAAAGCTGCGGAAGAAGCAGCGAAGAAAGCGGCTGCAGACGCAAAGAAAAAAGCAGAAGCAGAAGCCGCCAAAGCCGCAGCCGAAGCGCAGAAAAAAGCCGAGGCAGCCGCTGCGGCACTGAAGAAGAAAGCGGAAGCGGCAGAAGCAGCTGCAGCTGAAGCAAGAAAGAAAGCGGCAACTGAAGCTGCTGAAAAAGCCAAAGCAGAAGCTGAGAAGAAAGCGGCTGCTGAAAAGGCTGCAGCTGATAAGAAAGCGGCAGCAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCG  >  NC_000913/776740‑777197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑