Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	737,716	G→A	intergenic (+1017/‑376)	ybfQ → / → ybfD	inactive transposase YbfQ/H repeat‑associated putative transposase YbfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	737,716	0	G	A	100.0%	13.8 / NA	7	intergenic (+1017/‑376)	ybfQ/ybfD	inactive transposase YbfQ/H repeat‑associated putative transposase YbfD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/3);  total (4/3)

AAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGACTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTT  >  NC_000913/737506‑737955                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                aaGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGACTGAAGAAATTATGCATGGCAGTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTGCTGCTGATTTAACCGCAGAAAAGTTCGCCACAACAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTg                                                                                                                                                                                                         <  1:175067/251‑1 (MQ=255)               agaagaAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGACTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAACAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTgg                                                                                                                                                                                           <  1:46515/251‑1 (MQ=255)                                                                                                      ngTGGCAGTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAACAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTa                                                                                                     >  1:16406/2‑250 (MQ=255)                                                                                                                       ggTCAATAATAGAAGAACACAAGAAAGCACCAGAAATGACGGTCAGATATTATATCAGTTATGCTGATTTAACCGCAGAAAAGTTCGCCACAAAAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATACGTAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTc                                                                                   >  1:52579/1‑251 (MQ=14)                                                                                                                                   cAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAACAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAg                                                                            >  1:58913/1‑246 (MQ=255)                                                                                                                                                                    gATATTATATCAGTTCTGCTGATTTAACCGCAGAAAAGTTAGCCACAACAATCAGAAACCACTGCCACGTGGCGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGACTGAAGACGACTGCAACATAAGAAGAGGCAACGCCGCAGATTTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGCATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGAt                                         >  1:173013/1‑248 (MQ=14)                                                                                                                                                                                                        aGTTCGCCACAACAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCtn  <  1:43897/251‑2 (MQ=255)                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                AAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGACTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCAATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTT  >  NC_000913/737506‑737955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑