Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	702,796	G→A	intergenic (‑45/+15)	nagA ← / ← nagB	N‑acetylglucosamine‑6‑phosphate deacetylase/glucosamine‑6‑phosphate deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	702,796	0	G	A	100.0%	10.3 / NA	5	intergenic (‑45/+15)	nagA/nagB	N‑acetylglucosamine‑6‑phosphate deacetylase/glucosamine‑6‑phosphate deaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/4);  total (1/4)

CGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAG  >  NC_000913/702621‑703008                                                                                                                                                                                |                                                                                                                                                                                                                     cGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAAGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACtttn                                                                                                                                           <  1:17093/251‑2 (MQ=255)                                                                     aacCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAAGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGt                                                                       <  1:38154/251‑1 (MQ=255)                                                                       ccGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAAGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTcc                                                                     >  1:59367/1‑251 (MQ=255)                                                                                                       nnnnnncGNNNNNGGGNTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAAGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCa                                                                                                                                          <  2:108167/144‑1 (MQ=255)                                                                                                                                          ccccGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAAGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAg  <  1:57336/251‑1 (MQ=255)                                                                                                                                                                                |                                                                                                                                                                                                                     CGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAG  >  NC_000913/702621‑703008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑