Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	1,303,073	Δ12 bp	coding (175‑186/921 nt)	oppB →	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1303072	1 (0.020)	36 (0.570)	34/482	0.9	97.3%	coding (174/921 nt)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
	?	NC_000913	1303085 =	1 (0.020)					coding (187/921 nt)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

CCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATC  >  NC_000913/1302838‑1303321                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             gggcaggagtgtcctgctccacggtgtctgatttttatcgcattacagaaggcacaggccagaaggtagggcaatgttaaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacttactttaccgccagaagtgatggccaatatcgaagcgaaatatcagcttaatgatccgTAtt                                                                                                                                                                                                                                           <  2:38668‑M2/4‑1 (MQ=255)           gtcctgctccacggtgtctgatttttatcgcattacagaaggcacaggccagaaggtagggcaatgttaaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCt                                                                                                                                                                                                                                 <  1:84956‑M2/14‑1 (MQ=255)                   ccacggtgtctgatttttatcgcattacagaaggcacaggccagaaggtagggcaatgttaaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAaacaac                                                                                                                                                                                                                         <  2:20367‑M2/22‑1 (MQ=255)                                  ttaatcgcattacagaaggcacaggccagaaggtaggtcaatgttaaaatttattctacgtcgctggctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagccattttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatataatcttaatgatcagTATTTCAGCTACCTCAAACAAGTGgcgcacgcacatt                                                                                                                                                                                                          >  2:441020‑M2/215‑245 (MQ=255)                                         cattacagaaggcacaggccagaaggtagggcaatgttaaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTcc                                                                                                                                                                                                   <  2:97063‑M2/44‑1 (MQ=255)                                                                               aaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactattgcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTgg                                                                                                                                                             <  1:300105‑M2/82‑1 (MQ=255)                                                                                         ctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGGGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATcc                                                                                                                                                     >  1:490080‑M2/160‑249 (MQ=255)                                                                                                   gtctggaagcgattacgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGtt                                                                                                                                         >  1:575184‑M2/150‑251 (MQ=255)                                                                                                              attccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgtcgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACt                                                                                                                              <  1:258364‑M2/113‑1 (MQ=255)                                                                                                                 attCTTATTACTATTTCGTTCTTTATGATGCGCTTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCTCCAGAAGGGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTTAAACAACTGTCGCACGGTGATTGCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGAATCCAGTTTTCCCGTTTCTGCCaacatggg                                                                                                                           >  1:522212/1‑245 (MQ=255)                                                                                                                     cgctatttattcttattactatttcgttctttatgatgcgcctcgcgcagggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGcc                                                                                                                       >  2:614121‑M2/132‑251 (MQ=255)                                                                                                                              ttcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGc                                                                                                                                    <  2:291489‑M2/107‑1 (MQ=255)                                                                                                                              ttcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGc                                                                                                                                    >  1:291489‑M2/123‑229 (MQ=255)                                                                                                                                     ttntagttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgatcgtactttaccgccagaagttatggccaatatctaatcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTTGCGCACGGTGATTTCGGGCCTTCGTTTAAATATAAAGATTATTCGGTCAATTACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCTGCAGCATTCTTCCtt                                                                                                        <  2:599869‑M2/135‑1 (MQ=255)                                                                                                                                     tactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCtt                                                                                                        <  2:348126‑M2/135‑1 (MQ=255)                                                                                                                                      actatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGc                                                                                                      >  1:135290‑M2/115‑251 (MQ=255)                                                                                                                                        tatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAATTTTAAAGATTATTCGTTCAATGACTTGGTTGCATCCATTTTTCCCGTTTCTGCCAACCTGGGAGCCGCAGCATTTTTCCTTCCgg                                                                                                    >  2:246132‑M2/113‑251 (MQ=255)                                                                                                                                         atttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGt                                                                                                   <  1:3938‑M2/140‑1 (MQ=255)                                                                                                                                             cgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCTTTTCTGCCAAACTTGGAGCCGCAGCATTTTTCCTTGCGGTAATa                                                                                               <  1:614121‑M2/144‑1 (MQ=255)                                                                                                                                                tctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTg                                                                                            >  1:291740‑M2/105‑251 (MQ=255)                                                                                                                                                   ttatgatgcgcctcgcgccgggaaggccttttaccggcgaacgtactttaccgccagaagcgatggccaatatcgaagcgagatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTTGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGGTGCATCCAGTTTTCTCGTTTCTGCCAAACTGGGAGCCGCAGAATTTTTCATTGCGTTAATACTGGgt                                                                                         >  2:349656‑M2/102‑251 (MQ=255)                                                                                                                                                            gcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCt                                                                                <  1:246132‑M2/159‑1 (MQ=255)                                                                                                                                                                cgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatagcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCTTTTCTGTCAATCTGTGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTgg                                                                              <  1:79431‑M2/161‑1 (MQ=255)                                                                                                                                                                 gcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGt                                                                           <  2:537904‑M2/164‑1 (MQ=255)                                                                                                                                                                       ggangcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTGCCTGAAACAACTGGCGCGCGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGGTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGGGCTGGCGTGATTGc                                                                     <  2:592498‑M2/170‑1 (MQ=255)                                                                                                                                                                            cccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGtt                                                                          <  2:121413‑M2/165‑1 (MQ=255)                                                                                                                                                                            cccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGtt                                                                          >  1:121413‑M2/77‑241 (MQ=255)                                                                                                                                                                                gttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTTCCGGAAACAACTGGCGCACGGTGATTTCGGGCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTGTGCCTTGCGGTAATACTGGGTGTTAGTGCTGGCTTTATTGCCGCATTa                                                              <  2:385689‑M2/177‑1 (MQ=255)                                                                                                                                                                                   accggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCTCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTaaaaca                                                         <  1:539869‑M2/182‑1 (MQ=255)                                                                                                                                                                                     cggcaaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCGATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTGATTGCCGCATTaaaacaaa                                                       <  2:317534‑M2/184‑1 (MQ=255)                                                                                                                                                                                                    accgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGAc                                         <  2:490080‑M2/198‑1 (MQ=255)                                                                                                                                                                                                          agaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTTCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCg                                  <  2:342147‑M2/205‑1 (MQ=255)                                                                                                                                                                                                                                      tatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGGTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCACATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGggtgt      >  2:353449‑M2/19‑251 (MQ=255)                                                                                                                                                                                                                                       atcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGgttgtt     >  1:51990‑M2/18‑251 (MQ=255)                                                                                                                                                                                                                                         catcttaatgctccgTCTTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTcc                                                                                                          >  2:52637‑M2/16‑148 (MQ=255)                                                                                                                                                                                                                                         catcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTcc                                                                                                          <  1:52637‑M2/133‑1 (MQ=255)                                                                                                                                                                                                                                              taatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATc  <  2:223863‑M2/237‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             CCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATC  >  NC_000913/1302838‑1303321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.