Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,805,424 C→T G176D (GGC→GAC)  waaP ← lipopolysaccharide core heptose (I) kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,805,4240CT95.0% 65.7 / ‑6.0 20G176D (GGC→GAC) waaPlipopolysaccharide core heptose (I) kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (14/5);  minor base A (0/1);  total (14/6)
Fisher's exact test for biased strand distribution p-value = 3.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCGGCACAATAATCTTCAAGGCTAATTGTGGGAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTT  >  NC_000913/3805213‑3805645
                                                                                                                                                                                                                   |                                                                                                                                                                                                                             
cAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAAc                                                                                                                                                                                        <  2:268746/251‑1 (MQ=255)
               aaGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTa                                                                                                                                                                         <  1:242465/251‑1 (MQ=255)
                         atTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGt                                                                                                                                                               >  1:121886/1‑251 (MQ=255)
                                            cAAAATAAGCCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAAGTTTTAATTCATCTTCCCGGACAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCATCATa                                                                                                                                            <  2:352450/251‑1 (MQ=255)
                                               aaTAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGtt                                                                                                                                         >  1:252828/1‑251 (MQ=255)
                                                aTAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTg                                                                                                                                        >  2:77108/1‑251 (MQ=255)
                                                    aCCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAAAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAAATGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTac                                                                                                                                    >  1:401230/1‑251 (MQ=255)
                                                                 gCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGc                                                                                                                       >  1:129565/1‑251 (MQ=255)
                                                                  ccAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGc                                                                                                                       >  1:280172/1‑250 (MQ=255)
                                                                               tGCGTCAGGCCAATATTCCTTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACg                                                                                                         >  2:249442/1‑251 (MQ=255)
                                                                                 cGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGta                                                                                                       <  1:215837/251‑1 (MQ=255)
                                                                                                                                 aTCGCGCCAGCGGCGCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCGGCACAATAATCTTCAAGGCTa                                                        <  1:118545/250‑1 (MQ=255)
                                                                                                                                           cggcgCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCa                                                                                                                                                          <  1:64180/142‑1 (MQ=255)
                                                                                                                                           cggcgCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCa                                                                                                                                                          >  2:64180/1‑142 (MQ=255)
                                                                                                                                           cggcgCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCGGCACAATAATCTTCAAGGCTAATTGTGGGAgg                                             >  1:203296/1‑250 (MQ=255)
                                                                                                                                                             cGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCGGCACAATAATCTTCAAGGCTAATTGTGGGAGTGAGATCTTCGGtaataat                           >  2:12808/1‑251 (MQ=255)
                                                                                                                                                                                gATCGATAACTGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCGGCACAATAATCTTCAAGGCTAATTGTGGGAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAAt        >  1:108194/1‑251 (MQ=255)
                                                                                                                                                                                     aTAACTGAACTTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCGGCACAATAATCTTCAAGGCTAATTGTGGGAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGAtt   >  1:21117/1‑251 (MQ=255)
                                                                                                                                                                                     aTAACTGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCGGCACAATAATCTTCAAGGCTAATTGTGGGAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGAtt   >  2:92851/1‑251 (MQ=255)
                                                                                                                                                                                       aaCTGAAATTTTTAATTCATCTTCCCGGTCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCGGCACAATAATCTTCAAGGCTAATTGTGGGAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGAttt  >  1:274952/1‑250 (MQ=255)
                                                                                                                                                                                                                   |                                                                                                                                                                                                                             
CAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATATCTCTTTGCGTCAGGCCAATATTCATTGATGAGAAATATAAACCAATCAGGTCTTTATCGCGCCAGCGGCGCGGTACTTTTGCACGTATCTGTGCCCGATGCAGATCGATAACTGAAATTTTTAATTCATCTTCCCGGCCAGTAAATGGCAAATGAAGCAAAAAGTGGCAAATGTAACAGTCGCGGTGATTTATCCCTGCAGTATGCATTTTACGCACCATAGTTGCTACACGTGCGATCAGCATACGCTTAACACGTATATCAGGCGGGTTGACTGCCCAATCGGCACAATAATCTTCAAGGCTAATTGTGGGAGTGAGATCTTCGGTAATAATAAATGATGCGCGAGTTAATGGATTT  >  NC_000913/3805213‑3805645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑