Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,631,349	C→T	K414K (AAG→AAA)	yhiJ ←	DUF4049 domain‑containing protein YhiJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,631,349	0	C	T	100.0%	59.1 / NA	19	K414K (AAG→AAA)	yhiJ	DUF4049 domain‑containing protein YhiJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/12);  total (7/12)

CGGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACATTTGTGAACATGCTAATAGTGCTTCAAGTTTAGGTCTGTAATCTGTCGAAACAGAAAAATAACGATTAAAGGATCGACAAGAGATAGCCCGGCTAGTTTTAAACTTTTTGCCATTAATTATAGCATGATGCTTTTTATTGATAATTGCCGCTAATTCGACCGGATCGAGAGCATTGGTCATTTCATCAATAGAGACTGTTATCGGTCCTATAATGTAACGTTTTCGCTGATCATCAAAAGTTATCCCATGATGATTTGCCATTATTTTGTATTTGGGGTCATAGAAACATACAGGATAATCTTTTATCCCAGAGTAGGTATCCTCATCGAGTGGTTTCATACGCGTGAAGTTCTGCATATAGTTACCATTGCA  >  NC_000913/3631128‑3631591                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   cGGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACAATTGTGAACATGCTAATAGTGCTTCAAGTTTAGGTCTGTAATCGGTCGAAACAGAAAAATAACGATTAAAGGATCGACAAGAGATAGCCCGGCTAGTTTTAAACTTTTTGCCATTAATTATAGCATGATGTTTTTTATTGATAATTGCCGCTAATTCGAc                                                                                                                                                                                                                       <  1:135324/251‑1 (MQ=255)            aCCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACATTTGTGAACATGCTAATAGTGCTTCAAGTTTAGGTCTGTAATCTGTCGAAACAGAAAAATAACGATTAAAGGATCGACAAGAGATAGCCCGGCTAGTTTTAAACTTTTTGCCATTAATTATAGCATGATGTTTTTTATTGATAATTGCCGCTAATTCGACCGGATCgag                                                                                                                                                                                                              >  2:344276/1‑249 (MQ=255)                              ttCGCCTATTCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACATTTGTGAACATGCTAATAGTGCTTCAAGTTTAGGTCTGTAATCTGTCGAAACAGAAAAATAACGATTAAAGGATCGACAAGAGATAGCCCGTCTAGTTTTAAACTTTTTGCCATTAATTATAGCATGATGTTTTTTATTGATAATTGCCGCTAATTCGACCGGATCGAGAGCATTGGTCATTtcatca                                                                                                                                                                                           <  1:200954/250‑1 (MQ=255)                               tCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACATTTGTGAACATGCTAATAGTGCTTCAAGTTTAGGTCTGTAATCTGTCGAAACAGAAAAATAACGATTAAAGGATCGACAAGAGATAGCCCGGCTAGTTTTAAACTTTTTGCCATTAATTATAGCATGATGTTTTTTATTGATAATTGCCGCTAATTCGACCGGATCGAGAGCATTGGTCATTTCATCAAt                                                                                                                                                                                         >  1:139093/1‑251 (MQ=255)                                cGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACATTTGTGAACATGCTAATAGTGCTTCAAGTTTAGGTCTGTAATCTGTCGAAACAGAAAAATAACGATTAAAGGATCGACAAGAGATAGCCCGGCTAGTTTTAAACTTTTTGCCATTAATTATAGCATGATGTTTTTTATTGATAATTGCCGCTAATTCGACCGGATCGAGAGCATTGGTCATTTCATCAATa                                                                                                                                                                                        <  1:76946/251‑1 (MQ=255)                                                 ccaccaTTTCCGTTATGTGCAACAACTTGATTTATACCTAACATTTGTGAACATGCTAATAGTGCTTCAAGTTTAGGTCTGTAATCTGTCGAAACAGAAAAATAACGATTAAAGGATCGACAAGAGATAGCCCGGCTAGTTTTAAACTTTTTGCCATTAATTATAGCATGATGTTTTTTATTGATAATTGCCGCTAATTCGACCGGATCGAGAGCATTGGTCATTTCATCAATAGAGACTGTTATCGGTcc                                                                                                                                                                       <  1:2143/251‑1 (MQ=255)                                                     caTTTCCGTTATGTGCAACAACTTGATTTATACCTAACATTTGTGAACATGCTAATAGTGCTTCAAGTTTAGGTCTGTAATCTGTCGAAACAGAAAAATAACGATTAAAGGATCGACAAGAGATAGCCCGGCTAGTTTTAAACTTTTTGCCATTAATTATAGCATGATGTTTTTTATTGATAATTGCCGCTAATTCGACCGGATCGAGAGCATTGGTCATTTCATCAATAGAGACTGTTATCGGTCCtat                                                                                                                                                                    >  2:13491/1‑250 (MQ=255)                                                                                  ataCCTCAAATTTGTGAACACGCTCATAGCGCTTCAAGTTTAGGTCTGCAATCTGTCGAAACAGAAAAATAACGATTAAAGGATCGACCAGAGATAGCCCGGCTAGATTTAAACTTTTTGACATTAATTCTAGCATGCTGTTTTTTATTGATAATTGGCGCTCATTCGACCGGATCGAGAGCATTGGTCATTTCATCAATAGAGACTGTTATCGGTCATATAATGTAACGTTTTCGCTGATCATCAAAAGt                                                                                                                                      >  2:384400/1‑251 (MQ=255)                                                                                         aCATTTGTGAACATGCTAATAGTGCTTCAAGTTTAGGTCTGTAATCTGTCGAAACAGAAAAATAACGATTAAAGGATCGACAAGAGATAGCCCGGCTAGTTTTAAACTTTTTGCCATTAATTATAGCATGATGTTTTTTATTGATAATTGCCGCTAATTCGACCGGATCGAGAGCATTGGTCATTTCATCAATAGAGACTGTTATCGGTCCTATAATGTAACGTTTTCGCTGATCATCAAAAGTTATccc                                                                                                                                >  1:381080/1‑250 (MQ=255)                                                                                               gtgAACATGCTAATAGTGCTTCAAGTTTAGGTCTGTAATCTGTCGAAACAGAAAAATAACGATTAAAGGATCGACAAGAGATAGCCCGGCGAGTTTTAAACTTTTTTCCATTAATTATAGCATGATTTTTTTTATTGATAATTGCCGCTAATTCGACCAGATCGAGAGCATTGGTTATTTCATCAATAGAGGCTGTTATCTGTCCTATAATGTAACGTTTTCGCTGATCATCAAAAGTTATCCCatgatg                                                                                                                          <  1:254914/250‑1 (MQ=255)                                                                                                     aTGCTAATAGTGCTTCAAGTTTAGGTCTGTAATCTGTCGAAACAGAAAAATAACGATTAAAGGATCGACAAGAGATAGCCCGGCTAGTTTTAAACTTTTTGCCATTAATTATAGCATGATGTTTTTTATTGATAATTGCCGCTAATTCGACCGGATCGAGAGCATTGGTCATTTCATCAATAGAGACTGTTATCGGTCCTATAATGTAACGTTTTCGCTGATCATCAAAAGTTATCCCATGAGGATTTGcc                                                                                                                   <  1:81248/251‑1 (MQ=255)                                                                                                                           aGGTCTGTTCTCTCTCGAAACAGAACAATAACGATTCAAGCATCGACACGAGAAAGACCGGCTAGTTTAAAACTTTATACCATTAAATATAGCATGATGTTTTTTATTGATAATTGCCGCTAATTCGACCAGCTAGAGAGCATTGGTCATTTCATCAATAGAGACTGTTATCGGTCCTATAATGTAACGTTTTCGCTGATCATCAAAAGTTATCACATGATGATTTGCCATTATTTTGTATTTGGGGTCAt                                                                                             >  2:376721/1‑251 (MQ=255)                                                                                                                                                 gAAAAATAACGATTAAAGGATCGACAAGAGATAGCCCGGCTAGTTTTAAACTTTTTGCCATTAATTATAGCATGATGTTTTTTATTGATAATTGCCGCTAATTCGACCGGATCGAGAGCATTGGTCATTTCATCAATAGAGACTGTTATCGGTCCTATAATGTAACGTTTTCGCTGATCATCAAAAGTTATCCCATGATGATTTGCCATTATTTTGTATTTGGGGTCATAGAAACATACAGGATAATCttt                                                                       <  2:283432/251‑1 (MQ=255)                                                                                                                                                      aTAACGATTAAAGGATCGACAAGAGATAGCCCGGCTAGTTTTAAACTTTTTGCCATTAATTATAGCATGATGTTTTTTATTGATAATTGCCGCTAATTCGACCGGATCGAGAGCATGGGTCATTTCATCAATAGAGACTGTTATCGGTCCTATAATGTAATGTTTTCGCTGAGCATCAAAAGTTATCCCATGATGATTTGCCATCATTGTGCAGTCGGGGTCATAGAAACATACAGGATAATCTATTATcc                                                                  <  2:49823/251‑1 (MQ=255)                                                                                                                                                                       gACAAGAGATAGCCCGGCTAGTTTTAAACTTTTTGCCATTAATTATAGCATGATGTTTTTTATTGATAATTGCCGCTAATTCGACCGGATCGAGAGCATTGGTCATTTCATCAATAGAGACTGTTATCGGTCCTATAATGTAACGTTTTCGCTGATCATCAAAAGTTATCCCATGATGATTTGCCATTATTTTGTATTTGGGGTCATAGAAACATACAGGATAATCTTTTATCCCAGAGTAGGTATCCTCa                                                 <  2:55370/251‑1 (MQ=255)                                                                                                                                                                         cAAGAGATAGCCCGGCTAGTTTTAAACTTTTTGCCATTAATTATAGCATGATGTTTTTTATTGATAATTGCCGCTAATTCGACCGGATCGAGAGCATTGGTCATTTCATCAATAGAGACTGTTATCGGTCCTATAATGTAACGTTTTCGCTGATCATCAAAAGTTATCCCATGATGATTTGCCATTATTTTGTATTTGGGGTCATAGAAACATACAGGATAATCTTTTATCCCAGAGTAGGTATCCTCATc                                               <  1:73909/251‑1 (MQ=255)                                                                                                                                                                                 aGCCCGGCTAGTTTTAAACTTTTTGCCATTAATTATAGCATGATGTTTTTTATTGATAATTGCCGCTAATTCGACCGGATCGAGATCATTGGTCATTTCATCAATAGAGACTGTTATCGGTCCTATAATGTAACGTTTTCGCTGATCATCAAAAGTTATCCCATGATGATTTGCCATTATTTTGTATTTGGGGTCATAGAAACATACAGGATAATCTTTTATCCCAGAGTAGGTATCCTCATCGAGTGGtt                                       <  1:114746/251‑1 (MQ=255)                                                                                                                                                                                                                      gCATGATGTTTTTTATTGATAATTGCCGCTAATTCGACCGGATCGAGAGCATTGGTCATTTCATCAATAGAGACTGTTATCGGTCCTATAATGTAACGTTTTCGCTGATCATCAAAAGTTATCCCATGATGATTTGCCATTATTTTGTATTTGGGGTCATAGAAACATACAGGATAATCTTTTATCCCAGAGTAGGTATCCTCATCGAGTGGTTTCATACGCGTGAAGTTCTGCATATAGTTACCATTGCa  <  1:32096/251‑1 (MQ=255)                                                                                                                                                                                                                       cATGATGTTTTTTATTGATAATTGCCGCTAATTCGACCGGATCGAGAGCATTGGTCATTTCATCAATAGAGACTGTTATCGGTCCTATAATGTAACGTTTTCGCTGATCATCAAAAGTTATCCCATGATGATTTGCCATTATTTTGTATTTGGGGTCATAGAAACATACAGGATAATCTTTTATCCCAGAGTAGGTATCCTCATCGAGTGGTTTCATACGCGTGAAGTTCTGCATATAGTTACCATTGCa  >  2:231599/1‑250 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   CGGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACATTTGTGAACATGCTAATAGTGCTTCAAGTTTAGGTCTGTAATCTGTCGAAACAGAAAAATAACGATTAAAGGATCGACAAGAGATAGCCCGGCTAGTTTTAAACTTTTTGCCATTAATTATAGCATGATGCTTTTTATTGATAATTGCCGCTAATTCGACCGGATCGAGAGCATTGGTCATTTCATCAATAGAGACTGTTATCGGTCCTATAATGTAACGTTTTCGCTGATCATCAAAAGTTATCCCATGATGATTTGCCATTATTTTGTATTTGGGGTCATAGAAACATACAGGATAATCTTTTATCCCAGAGTAGGTATCCTCATCGAGTGGTTTCATACGCGTGAAGTTCTGCATATAGTTACCATTGCA  >  NC_000913/3631128‑3631591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑