Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,496,203	A→G	K731K (AAA→AAG)	nirB →	nitrite reductase (NADH) large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,496,203	0	A	G	100.0%	203.1 / NA	60	K731K (AAA→AAG)	nirB	nitrite reductase (NADH) large subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/25);  total (35/25)

GTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAATATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAGCGGTACTGTGTGAGTGGACTGAAACGGTCAATACGCCGTCTGCGCAGACTCGCTTCAAACACTTCATCAAC  >  NC_000913/3495973‑3496446                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    gTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAA‑GACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCCCGTC‑ATGCGGA‑TCTGATGGCGGAGGATATAGATAGCGAAACGCTGATCAAGGCTCTCGACCGCTTCctgatg                                                                                                                                                                                                                                >  2:277191/1‑251 (MQ=255)   cGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCatgatg                                                                                                                                                                                                                                <  1:183240/250‑1 (MQ=255)             gTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCg                                                                                                                                                                                                                     <  2:79972/251‑1 (MQ=255)               ggtaCTGGAAACCCGCTACAAATGCATCCGTACGCCGCATCAAATTAAGTTCGTTTTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGTATCCTGTATGTTTACTGTAACTGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCTGATATCGATCGCGCAACGCTGATCAAGTATCTCGATCGCTTCACGATGCTCTActcccgta                                                                                                                                                                                                                   <  2:224290/248‑8 (MQ=255)                gAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTAc                                                                                                                                                                                                                  >  1:394629/1‑251 (MQ=255)                              cTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTg                                                                                                                                                                                                     >  2:299239/1‑250 (MQ=255)                                aCAAAGGCGTCCGTTCGCCGCACAAAATGAAGTGCGGTGTATCCGGCTGTTCCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTTTTATCGCCACTGAAAAAGGCGGGACCCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGTGGCTGATATCGATCGCGAATCGCTGATGAAGTATCTCGAACGCTTCATGATGTTCTACATCCGTGCTGCCGACAAACTGa                                                                                                                                                                                                    <  2:240412/249‑1 (MQ=255)                                aCAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGAcg                                                                                                                                                                                                  <  1:212556/251‑1 (MQ=255)                                    aGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGAACTGCCGACAAACTGACGCGt                                                                                                                                                                                               >  1:202632/1‑250 (MQ=255)                                             tACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGGATCTAGACCGCTTTATGATGTTCTACATCCGTACTGCCGACAAACTGACGGGTACCGCACCGt                                                                                                                                                                                     >  1:275332/1‑251 (MQ=255)                                              aCGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTg                                                                                                                                                                                    >  1:41836/1‑251 (MQ=255)                                                   gcACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGtt                                                                                                                                                                                 >  2:23474/1‑249 (MQ=255)                                                    cacaAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGtt                                                                                                                                                                                 >  1:199986/1‑248 (MQ=255)                                                       aaaaggaatgtagttgtCTCCGGCTGTACTCGTTAATGCTCAGAATCCAAGGCTAATGCAGTGGGTAGTATCGTCAATGAAAAAGGCTGGAACCCGTATGTTTGCGGTAACGGCTGCATCACACCGCGCC‑ATGCGGA‑TCTGTTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTAGCCTGCTTCTTCATGTTTTGCATCAGTACTGCCGGTAAACTGACGCGTACCGTACCGTGGTTAGaaac                                                                                                                                                                           <  2:312535/237‑2 (MQ=255)                                                         aaTNAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAAcc                                                                                                                                                                         <  2:366298/251‑1 (MQ=255)                                                             aaGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGAGGTTAGAAAACCTCg                                                                                                                                                                      >  2:99341/1‑250 (MQ=255)                                                                 tCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGGATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGGTAGAAAACCTCGAAggc                                                                                                                                                                 >  2:266800/1‑251 (MQ=255)                                                                    gtgtCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAAACGCGTC‑ATGCGGA‑TCTGCTTGCGGCGGATATCGATCGCGAAACGCTGATCACGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTCGAAAACCTCTAAggnggc                                                                                                                                                              >  2:368595/1‑251 (MQ=255)                                                                          ccGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATc                                                                                                                                                           <  2:393519/248‑1 (MQ=255)                                                                            ggCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGC‑TCTGCTGGCGGCGGATATCGATCGGGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGAACTGCCGACAAACTGACGCGTACGGCCCCGGGGTTAGAAAACCTCGCAGGCGGCATAGAtt                                                                                                                                                       >  1:386939/1‑250 (MQ=255)                                                                            ggCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTa                                                                                                                                                      <  1:43031/251‑1 (MQ=255)                                                                                    ccgcgAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGACACAGGATGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACTGCGTC‑ATGCGGAATC‑GCTGGCGGCGGATATCCATCGAGCAACGCTGATCAAGTATCTCGGCCTCTTCATGATGTTCTACATCCGTACTGGCGACAAACTGACGCGTACCGCACCGTGGTTAGACAACCTCGACGGCGGCACCGATCATCTGaa                                                                                                                                                >  2:68643/5‑249 (MQ=255)                                                                                        gAATGTTCAGCAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGCC‑ATGCGGG‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCCCGAAGGCGGCATCGATTACCTGAAAGCAgg                                                                                                                                          >  2:254570/1‑250 (MQ=255)                                                                                            gTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGAGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATc                                                                                                                                      >  2:289871/1‑251 (MQ=255)                                                                                             tttaggaGCTCAGGGTAAAGACGTGGCAATTATCGACACTGAAAAAGGCTCGAACCTGAATGTTTGCGGTAACGGCGCCCTGAAACCGCGTCCATG‑GGC‑TCTGCTGGCGCCGGCTATCGCTCGCGAAACTCTGATTCAGTATCTCGAGCGCTTCATGATGTTCTGCATCCGTACTGCCGCCAAACTGGCGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCTTCGATTACCTGAAAGCAGGGNTc                                                                                                                                      >  2:360976/7‑250 (MQ=255)                                                                                               cAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCAt                                                                                                                                    <  1:203763/250‑1 (MQ=255)                                                                                                aGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGGGGCATCGATTACCTGAAAGCAGTGATCATTg                                                                                                                                  <  1:102479/251‑1 (MQ=255)                                                                                                   aGCTCATGGTAAAGACGTGGGTATTATAGCCACTAAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAACCCGTGTA‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGTCGGCATCGATTAACTGAAAGCAGTGATAATTgacg                                                                                                                               <  1:301119/251‑1 (MQ=255)                                                                                                       cAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACa                                                                                                                            >  1:203722/1‑250 (MQ=255)                                                                                                        aGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACaa                                                                                                                           >  1:372773/1‑250 (MQ=255)                                                                                                        aGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACaa                                                                                                                           >  1:341124/1‑250 (MQ=255)                                                                                                             aaaGTCGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTgg                                                                                                                      <  2:307346/250‑1 (MQ=255)                                                                                                                                      aaaaaGGATCGAACATGTCTGTTTGCGGTAACGGCGGCCTGAAACCGCGTC‑CTGCGGA‑TATGCTGGCCGCGGATATCGATCGAGACACGCTGATCAAGTCTCTCGCCCGCTTCATGCTGTTCTCCATCCGTACTGCCGACACACTGCCGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGGGATCATTGACGACAAACTGGGGCTGACCGCACATCTGGcagang                                                                                              >  2:357020/1‑249 (MQ=255)                                                                                                                                       aaaaGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGaagaag                                                                                              >  1:118211/1‑248 (MQ=255)                                                                                                                                       aaaaGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAgaga                                                                                           <  1:23474/251‑1 (MQ=255)                                                                                                                                        aaaGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAGACCGCGCA‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGGATCTCGACCGCTTCATGATGTTCTACATCCGGACGGCCGACAAACTGACGCGAACCACACCGTGGGTAGAAAACCTCGACGGCGGCATCGATTACCTGAAAGCAGTGGTCATTGAGGACAGACTGGGGCTGAACGCACACCTGGAAGAAgaga                                                                                           >  1:289203/1‑250 (MQ=255)                                                                                                                                         aaGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATg                                                                                         <  1:152570/251‑1 (MQ=255)                                                                                                                                              tGGAACATGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑AGGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAAAGGTGATCAAGTATCTCGACCGCTTCATGATGCTTTACATCCGTACTGCCGACAACCTGACACGTACCGCACCGTCGTTAGACAAGCTCGAAGGCGGCATCGATTACCTGAAAGCCGGGCACATTGACGACCAACTGGGGCTAAACTCAAACCTGGAAGAAGAGATggggcc                                                                                    >  2:193972/1‑247 (MQ=255)                                                                                                                                              gggAACCTGTATGTTTGCGGGAACGGCGGCATGAAACCGCGTG‑AGGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACGGACGCGTACCGCTCCGTGGTTAGAAAACCTCGATGGCGGCATCGATTACCTGGATGCAGTGATCATTGACGACAGACTGGGTCTGAACGCACAGCTGGAAGAAGATATGgcgcg                                                                                    <  2:23816/250‑1 (MQ=255)                                                                                                                                                  aCCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGCAGAGATGGCGCGCCTg                                                                                >  1:73797/1‑251 (MQ=255)                                                                                                                                                          gTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAg                                                                         <  2:84958/250‑1 (MQ=255)                                                                                                                                                          gTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAg                                                                         <  1:99341/250‑1 (MQ=255)                                                                                                                                                          gTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAGc                                                                        <  1:299239/251‑1 (MQ=255)                                                                                                                                                                 gTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGATCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAGCGGTACtg                                                                 <  2:237663/251‑1 (MQ=255)                                                                                                                                                                  tAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTg                                                                            >  1:60542/1‑239 (MQ=255)                                                                                                                                                                  tAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTg                                                                            <  2:60542/239‑1 (MQ=255)                                                                                                                                                                      ggcggcATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATTGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTATACATCCGTAATGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAAACTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAGCGGTACtgtgtg                                                             >  1:181152/1‑250 (MQ=255)                                                                                                                                                                                      gTC‑ATGCGGA‑TATGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGACAGCAGTGATCATTGACGACAAACTGGCGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGAGAAGCGGTACTGTGTGGGTGGACTGAAACGGTc                                            >  2:213672/1‑251 (MQ=255)                                                                                                                                                                                        gtcatgcgggcatgctgGCGGCGGATATCGATCGCGAAACGCTGATCAAGTATCACGACCGCTTCATGATGGTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAAGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACGGGGGCTGACCGCACAGCTGGAAGAAGAGATGGCGCGCCTTCGTGAAGCGGTACTGTGTGTGTGGTCTGAAACGGTc                                            <  2:386939/239‑1 (MQ=255)                                                                                                                                                                                          aTGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGGAACGCTGATCAAGTATCTCGACCGCTTCATGATTTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAGCCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAGCGGTACTGTGTGAGTGGACTGAAACGGTCAAt                                         <  1:1858/251‑1 (MQ=255)                                                                                                                                                                                                          cggcggATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAGCGGTACTGTGTGAGTGGACTGAAACGGTCAATACGCCGTCTGCGCAg                          <  1:401423/251‑1 (MQ=255)                                                                                                                                                                                                           ggcggATATCGATCGCGAAAAGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAGCGGTACTGTGTGAGTGGACTGAAACGGTCAATACGCCGTCTgcgc                            >  2:12638/1‑248 (MQ=255)                                                                                                                                                                                                               gATATCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAGCGGTACTGTGTGAGTGGACTGAAACGGTCAATACGCCGTCTGCGCAGACTCg                     >  1:120099/1‑251 (MQ=255)                                                                                                                                                                                                                atatCGATCGCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAGCGGTACTGTGTGAGTGGACTGAAACGGTCAATACGCCGTCTGCGCAGACTCGc                    >  1:65799/1‑251 (MQ=255)                                                                                                                                                                                                                  atcgatcgCGAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAGCGGTACTGTGTGAGTGGACTGAAACGGTCAATACGCCGTCTGCGCAGACTCGCtt                  >  1:43156/1‑251 (MQ=255)                                                                                                                                                                                                                           gAAACGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAGCGGTACTGTGTGAGTGGACTGAAACGGTCAATACGCCGTCTGCGCAGACTCGCTTCAAACACtt         >  2:122985/1‑251 (MQ=255)                                                                                                                                                                                                                              aCGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAGCGGTACTGTGTGAGTGGACTGAAACGGTCAATACGCCGTCTGCGCAGACTCGCTTCAAACACTtcat      <  2:341124/251‑1 (MQ=255)                                                                                                                                                                                                                               cGCTGATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAGCGGTACTGTGTGAGTGGACTGAAACGGTCAATACGCCGTCTGCGCAGACTCGCTTCAACCACTtcatc     >  2:1319/1‑251 (MQ=255)                                                                                                                                                                                                                                gCTGATTAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAGCGGTACTGTGTGAGTGGACTGAAACGGTCAATACGCCGTCTGCGCAGACTCGCTTCAAACACTtcatc     >  1:309864/1‑250 (MQ=255)                                                                                                                                                                                                                                   gATCAAGTATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGAAAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAGCGGTACTGTGTGAGTGGACTGAAACGGTCAATACGCCGTCTGCGCAGACTCGCTTCAAACACTTCATCAAc  >  1:239678/1‑250 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    GTCGGCCTCGGCGTGGAACTGGAAAACCGCTACAAAGGCATCCGTACGCCGCACAAAATGAAGTTCGGTGTCTCCGGCTGTACCCGTGAATGTTCAGAAGCTCAGGGTAAAGACGTGGGTATTATCGCCACTGAAAAAGGCTGGAACCTGTATGTTTGCGGTAACGGCGGCATGAAACCGCGTC‑ATGCGGA‑TCTGCTGGCGGCGGATATCGATCGCGAAACGCTGATCAAATATCTCGACCGCTTCATGATGTTCTACATCCGTACTGCCGACAAACTGACGCGTACCGCACCGTGGTTAGAAAACCTCGAAGGCGGCATCGATTACCTGAAAGCAGTGATCATTGACGACAAACTGGGGCTGAACGCACATCTGGAAGAAGAGATGGCGCGCCTGCGTGAAGCGGTACTGTGTGAGTGGACTGAAACGGTCAATACGCCGTCTGCGCAGACTCGCTTCAAACACTTCATCAAC  >  NC_000913/3495973‑3496446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑