Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,205	C→A	T29K (ACG→AAG)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,205	0	C	A	97.7%	143.1 / NA	45	T29K (ACG→AAG)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (21/22);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTA  >  NC_000913/3485959‑3486446                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   aCAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCtt                                                                                                                                                                                                                                                >  1:121534/1‑251 (MQ=255)   attAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTAGAAAGCCCCTTCCCAGGTAGGGGGAAGCATATTGCGGCAACCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGGGGATAACCGCGCATGGTGCTTGGCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTAt                                                                                                                                                                                                                                              <  2:389507/249‑1 (MQ=255)          cATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCAcc                                                                                                                                                                                                                                         >  1:34289/1‑249 (MQ=255)                 gTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATATCCCCTTCCTAGGTAGCGGGAAGCTTATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAGCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑Gaaa                                                                                                                                                                                                                               <  1:28533/251‑1 (MQ=255)                    cTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑Gaaaa                                                                                                                                                                                                                              >  2:399383/1‑249 (MQ=255)                           tATGCACAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCCTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGA‑GAAAACGCGGAAAc                                                                                                                                                                                                                     >  2:170109/1‑251 (MQ=255)                                aaaGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGCTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGG‑GAAAAAGCGGAAACGCTGt                                                                                                                                                                                                                >  1:11551/1‑251 (MQ=255)                                              tACCGTGCAGTACAGTGGATAGCCCCTTCCCAGGTAGCGGTAAGCATATTTCGGCAATCCAGATACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGTATAACCGCGCATGGTGCTTGGCAATCCGCAACCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTaa                                                                                                                                                                                                   <  1:319039/250‑1 (MQ=255)                                               aCCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCGCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTaaa                                                                                                                                                                                                  <  1:67220/250‑1 (MQ=255)                                                  gTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGct                                                                                                                                                                                              >  1:256024/1‑251 (MQ=255)                                                    gCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGCGAAGCATATTCCGGAAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctct                                                                                                                                                                                            <  1:75599/251‑1 (MQ=255)                                                     cAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtg                                                                                                                                                                                           >  2:133257/1‑251 (MQ=255)                                                      aGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGc                                                                                                                                                                                                                           <  1:11306/218‑1 (MQ=255)                                                      aGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGc                                                                                                                                                                                                                           >  2:11306/1‑218 (MQ=255)                                                              tGATAGCCCCTCCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAAAAAGCTTATAACAGAGGATACCCGCGCAGGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACTCTGTACTACATCGTTAAAGGCTCTGTGGCAGTg                                                                                                                                                                                   <  1:28263/250‑1 (MQ=255)                                                               gATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCt                                                                                                                                                                                 >  1:77148/1‑251 (MQ=255)                                                                        ttCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCan                                                                                                                                                                           >  2:361312/1‑247 (MQ=255)                                                                               gTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAAACGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAag                                                                                                                                                                   >  1:47369/1‑249 (MQ=255)                                                                                 aGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAggg                                                                                                                                                               >  1:149920/1‑251 (MQ=255)                                                                                    gggAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTaa                                                                                                                                                            <  2:77148/251‑1 (MQ=255)                                                                                                     aaTCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCt                                                                                                                                           >  2:236318/1‑251 (MQ=255)                                                                                                            agaCAGCGGCGTTATCGGCCTCTGGAGAAAGCTTATAAAAGAGGATAACCGCGCATGGTGCTAGGCAAACCGAAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCAATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGTCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGaa                                                                                                                                    <  2:298366/251‑1 (MQ=255)                                                                                                                agctgcgtcagcTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGTTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTGGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAg                                                                                                                                <  2:315253/240‑1 (MQ=255)                                                                                                                                     agaNAGCTTATAACAGAGGATAACCGCGCAGGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGACCTg                                                                                                           <  2:353803/251‑1 (MQ=255)                                                                                                                                       aaaGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTggg                                                                                                         >  2:245847/1‑251 (MQ=255)                                                                                                                                         aGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGcc                                                                                                       <  2:70103/251‑1 (MQ=255)                                                                                                                                                   agagGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCCTTGCCAAATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGACTGTTTGa                                                                                                >  1:368307/1‑248 (MQ=255)                                                                                                                                                                gcATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCATGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGt                                                                                <  1:72990/251‑1 (MQ=255)                                                                                                                                                                            gCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTa                                                                    <  2:256024/251‑1 (MQ=255)                                                                                                                                                                               aaCCGCAAACGGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATGCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGt                                                                 <  1:391772/251‑1 (MQ=255)                                                                                                                                                                               aaCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGt                                                                 <  2:149920/251‑1 (MQ=255)                                                                                                                                                                                 acGCAAGCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGAGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGc                                                               >  2:92789/2‑251 (MQ=255)                                                                                                                                                                                             ccGACGCTCGAATTGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATGCACCAGGGT‑GAAAGAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGAAGTGCTGATCAAAGACGAAGAGGGTAAAGAACTGATCATCTCCTATCCTAATCAGGGTGATTTGATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGAAACGTAGCCCATGGGTAAGTGCGAAAGCCGCCtt                                                   <  2:334382/251‑2 (MQ=255)                                                                                                                                                                                               gACTCTCGAATGTTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAACCTTATACCCCAGGGT‑AAAAAAGTGAAAACGCTGTATTACATCGTTAAAGGCTCTTTGGCAGTGCTGATCAAAGACAAAGAGTGTAAAGAAATGACCCCCTCCTATCTGAATCAGGTTGTTTTTTTTGGCGAACTGGGCCGGTTGAAATAGTCCCAGGAACGTAGCCCATGGGTGCGTGCGAAAACCGCCtgtg                                                 <  1:357038/251‑1 (MQ=255)                                                                                                                                                                                                   ctcGCATGGATCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGGAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAgg                                             >  2:44938/1‑250 (MQ=255)                                                                                                                                                                                                       aaTGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGc                                                               >  2:179233/1‑229 (MQ=255)                                                                                                                                                                                                       aaTGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGc                                                               <  1:179233/229‑1 (MQ=255)                                                                                                                                                                                                          ggTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGCAGTGGCTGa                                       >  2:145295/1‑250 (MQ=255)                                                                                                                                                                                                                tGTCTAATTGCCACATTAAAAAGTACCCATCAAAGAGAAAGTTTATCCACCAGGGT‑AAAAAAGCGAAACCGTTGTACTACATCGTTAAAGGCTCTGTGGGAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGTTCCTCTCCTATCTGAATCAGGGTGATTTTATTGGGGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGTTCCGTGCGAAAACCGCCTGTGAAGTGGCTGAAAtt                                   <  1:108862/248‑1 (MQ=255)                                                                                                                                                                                                                           cccaTTCATAAGTACCCATCCAAGAGCATGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGATGGTAAAGAAATGACCCTCTCCGATCTGAAGCAGGGTGATTTTATTGGCGAACTGAGACTTTTTGAAGAGGGCCAGGAACGTATCGCATGGCTACGTg                                                                <  2:40311/206‑1 (MQ=255)                                                                                                                                                                                                                                tCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGAGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTACTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGGATGGGCACGTGCGAAAACAGCCTGTGAAGTGGCTGAAATTTCGAACAAAAAATTTAGcc                >  2:272204/1‑251 (MQ=255)                                                                                                                                                                                                                                 aaaaaGTACCCAACCAAGAGCAAGCTTATTCACCAGGGT‑AAAAAAGCGGAAACGCTGTACAACATTTTTAAAGGCTCTGGGGCAGTGTTGATAAAAGACGAAGGGGGAAAAAAAATAATCCTCTCCTATCTGAATCAGGGTGTTTTTATTGGCGAACTGGGCCTGTTTAAAGAGGGCCAGAAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGcc                <  1:245847/247‑1 (MQ=255)                                                                                                                                                                                                                                              ccatgagCAAGCTTATTCACC‑GGGTATAAAAATCTGAAGCGCTGTATTACAGTGTTAAATGCTCTTTGTCATGGCGTATAAAAGACGAAGAGGGTAAAGAATTGTTCCTCTGCTATCTGAATCAGGTTGATGTTATTGGGGTTTTGTTCGTTTTTGAGGAGGGCCAGAAACGTATCTCATGGGTCCTTGAGAAAACCGCCTGTTAATTTGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTa  <  1:396300/247‑1 (MQ=255)                                                                                                                                                                                                                                              ccAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCCGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGGGGGCCAGGAACGTAGAGCATGGGTACGTGCGAAAACCGCCTGTGAAGCGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCANGt   >  2:357125/1‑250 (MQ=255)                                                                                                                                                                                                                                               cAAGAGCAAGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTa  >  1:22457/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   ACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGT‑GAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTA  >  NC_000913/3485959‑3486446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑