Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,450,107	C→T	A43T (GCG→ACG)	rplV ←	50S ribosomal subunit protein L22

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,450,107	0	C	T	100.0%	159.3 / NA	44	A43T (GCG→ACG)	rplV	50S ribosomal subunit protein L22
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/28);  total (16/28)

ATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCTTTTTTATCAGCAGCGTGGCCGCGATAAGTACGAGTCGGTGCGAATTCACCCAGTTTGTGACCAACCATTTCGTCGGTTACAAATACC  >  NC_000913/3449866‑3450350                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    aTGTACTTGCTGACCCATTGCTAGTCTCCAGAGTCTCTGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTGCGTCTACGAACATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCttgtt                                                                                                                                                                                                                                            <  1:255846/251‑1 (MQ=255)        ttCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGgtgt                                                                                                                                                                                                                                       <  1:68748/249‑1 (MQ=255)        ttCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGgtgt                                                                                                                                                                                                                                       >  1:19179/1‑249 (MQ=255)                   tGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTTAAAATATc                                                                                                                                                                                                                          >  1:98135/1‑251 (MQ=255)                    gCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATcc                                                                                                                                                                                                                         >  1:12592/1‑251 (MQ=255)                       aGTCTCCAGAGTCTCAGCGATCGGGCACAACCACAGTGATGTGGCTGGTTCGCTGCAGGATGCGATCTGCACGACCTTTTGCACGCTGCATAATGCGCTTCATGCTCGGTCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAGCTTTCTGGACCAGTACAGCCGTTTTCTTGTTTGTGTAGGTCAAAATATCCaga                                                                                                                                                                                                                      <  2:372199/251‑1 (MQ=255)                        gtgtcCAGAGGCTTAGCGATCGGACACAGCCACAGGGATGTGGCTGGTGCGCTTCAGGATGCGATCTACACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCACCGGGATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGGTGGTGTAGGTCAAAATATCCagag                                                                                                                                                                                                                     <  2:317488/248‑1 (MQ=255)                                agTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGac                                                                                                                                                                                                             <  2:135017/251‑1 (MQ=255)                                 gTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGac                                                                                                                                                                                                             >  1:301745/1‑250 (MQ=255)                                     cAGCGATCGGACACAACCATAGTGATGTGGCTGTTGCCCTTCATGATGCGATCTGCTCGACCTTTTGCACGCGGCATAATGCGCTTTATGCTCGGGCCTTCGTCTACGAAAATGTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCCATGGCATATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTTTTGGTGTAGGTCAAAATATCCAGAGCCTGCGacacc                                                                                                                                                                                                          <  2:337723/249‑2 (MQ=255)                                                   aaCCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATc                                                                                                                                                                                           >  1:100489/1‑250 (MQ=255)                                                               gtgGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTGCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGcaaca                                                                                                                                                                               <  1:394636/250‑1 (MQ=255)                                                                 ggCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAg                                                                                                                                                                             <  1:312397/250‑1 (MQ=255)                                                                               aGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGc                                                                                                                                                              <  2:277007/251‑1 (MQ=255)                                                                                ggATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGc                                                                                                                                                              >  1:256127/1‑250 (MQ=255)                                                                                  aTGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGc                                                                                                                                                              >  1:209379/1‑248 (MQ=255)                                                                                      gATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCCTGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCagaaga                                                                                                                                                        >  2:259922/1‑250 (MQ=255)                                                                                               gACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATgg                                                                                                                                              <  2:306898/251‑1 (MQ=255)                                                                                                     ttGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGt                                                                                                                                        >  1:143510/1‑251 (MQ=255)                                                                                                       gCACGCGGCATAATGCGCTTCATGCTCGGGCTTTCGTCTACGAAAATTGTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATTGCAGATTCCAGAACTTTCTTTACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATAGCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAATAAGTCGAACCTTCTGAGCAGAAGAACGAGCATGGCGAtttt                                                                                                                                       <  2:377293/250‑4 (MQ=255)                                                                                                       gCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGTCGATGtt                                                                                                                                       <  1:352259/250‑1 (MQ=255)                                                                                                       gCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGtt                                                                                                                                       <  2:100489/250‑1 (MQ=255)                                                                                                          cgcnGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGc                                                                                                                                   <  2:367296/251‑1 (MQ=255)                                                                                                                 tAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGtt                                                                                                                            >  1:292428/1‑251 (MQ=255)                                                                                                                          tCATGCTCGGGCCTTCGGCTACGAAAATTTTCGTAACTTTCATATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCAtctc                                                                                                                    <  2:290595/250‑1 (MQ=255)                                                                                                                                             tACGAAAATTTTCGAAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGAGGTTTAGCGATAGTTTCCATCTCTTCCTCCTCCCTTATttctt                                                                                                >  2:256216/1‑251 (MQ=255)                                                                                                                                               cGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATttcttct                                                                                              >  1:216650/1‑251 (MQ=255)                                                                                                                                                        tCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCtttt                                                                                      <  1:355020/250‑1 (MQ=255)                                                                                                                                                        tCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCtttt                                                                                      <  2:256127/250‑1 (MQ=255)                                                                                                                                                            aaCTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGACCTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACTCTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCTTTTTTAt                                                                                  <  2:84611/250‑1 (MQ=255)                                                                                                                                                            aaCTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCTTTTTTATc                                                                                 <  1:367973/251‑1 (MQ=255)                                                                                                                                                                 tCAGATCGTCAATGTCATCGCAATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTATTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTCTCTTACCGCGAATCAGGTCAGCAACAAGGCGATCCTTCTGAGCAGAAGAACGAGCATGTCGATGTTTAGCGATAGTTTCCATCCCGTCCTCCTACCTTATTTCTTCTTCGCTTTTTTATcagca                                                                             <  2:15805/250‑1 (MQ=255)                                                                                                                                                                  cAGATCGTCAATGTCAGCGCCATCGTTGTTTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCTTTGTTATcagcag                                                                            <  2:373604/250‑1 (MQ=255)                                                                                                                                                                                 aGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACCTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTTTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCTTTTTTATCAGCAGCGTGGCCGCTATAAGt                                                            <  1:368959/251‑1 (MQ=255)                                                                                                                                                                                   cgcCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCTTTTTTATCAGCAGCGTGGCCGCGATAAGTAc                                                          <  2:216650/251‑1 (MQ=255)                                                                                                                                                                                         cGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCTTTTTTATCAGCAGCGTGGCCGCGATAAGTACGAGTCg                                                    <  1:189335/251‑1 (MQ=255)                                                                                                                                                                                                         aGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCTTTTTTATCAGCAGCGTGGCCGCGATAAGTACGAGTCGGTGCGAATTCAccc                                      >  1:344235/1‑249 (MQ=255)                                                                                                                                                                                                               ggCAGATTCCAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCTTTTTTATCAGCAGCGTGGCCGCGATAAGTACGAGTCGGTGCGAATTCACCCAGTTtgt                               <  1:158376/250‑1 (MQ=255)                                                                                                                                                                                                                      tCCAGAACTTTCTTTACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAGCCTTCTGAGCATAAGAACGAGCATGGCGATGTTTAGCGATAGTGTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCTTTTTTATCAGCAGCGTGGCCGCGATAAGTACGAGTCGGTGCGAATTCACCCAGTTTGTGaccaac                        <  2:100511/250‑1 (MQ=255)                                                                                                                                                                                                                        cAGAACTTTCTTGACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCTTTTTTATCAGCAGCGTGGCCGCGATAAGTACGAGTCGGTGCGAATTCACCCAGTTTGTGACCAACCAt                     >  1:321615/1‑251 (MQ=255)                                                                                                                                                                                                                                    gACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCTTTTTTATCAGCAGCGTGGCCGCGATAAGTACGAGTCGGTGCGAATTCACCCAGTTTGTGACCAACCATTTCGTCGGTTAc         >  1:150780/1‑251 (MQ=255)                                                                                                                                                                                                                                    gACCAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCTTTTTTATCAGCAGCGTAGCCGCGATAAGTACGAGTCGGTGCGAATTCACCCAGTTTGTGACCAACCATTTCGTCGGTTa          <  2:301745/250‑1 (MQ=255)                                                                                                                                                                                                                                       cAGTACAGCCGTTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCTTTTTTATCAGCAGCGTGGCCGCGATAAGTACGAGTCGGTGCGAATTCACCCAGTTTGTGACCAACCATTTCGTCGGTTACaaa      <  2:120398/251‑1 (MQ=255)                                                                                                                                                                                                                                           aCAGCCGTTTTCTTGTTGGTGTAGGTCCAACTATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCTTTTTTATCAGCAGCGTGGCCGCGATAAGTACGAGTCGGTGCGAATTCACCCAGTTTGTGACCAACCATTTCGTCGGTTACAAATAcc  >  1:293300/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    ATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTCGCTTTTTTATCAGCAGCGTGGCCGCGATAAGTACGAGTCGGTGCGAATTCACCCAGTTTGTGACCAACCATTTCGTCGGTTACAAATACC  >  NC_000913/3449866‑3450350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑