Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,355,173	C→T	P150L (CCA→CTA)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,355,173	0	C	T	100.0%	157.2 / NA	48	P150L (CCA→CTA)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/16);  total (32/16)

GTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTT  >  NC_000913/3354951‑3355419                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       gTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGCATACAGATCCTGAACTCGCAGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGGGCTGTACCAACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTNATc                                                                                                                                                                                                                            >  2:367676/1‑251 (MQ=255)   ttacaaaaaaaCTACGCTGGCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCGAGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGAGATGGAGCGCCGTCTGTTTATcg                                                                                                                                                                                                                           <  2:379587/245‑1 (MQ=255)               tACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAg                                                                                                                                                                                                                                           >  1:249581/1‑222 (MQ=255)               tACGCTGTCGGGATGCTCTTCCTAAATAAAGACCCTGACCTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAg                                                                                                                                                                                                                                           <  2:249581/222‑1 (MQ=255)                 cGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTg                                                                                                                                                                                                            >  2:94947/1‑251 (MQ=255)                             gCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCg                                                                                                                                                                                                >  1:169866/1‑251 (MQ=255)                             gCTCTTCCTGAATAAAGATCCTGAACTAGCCGCTGCCGCACGCCGCATCGATGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCATCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCg                                                                                                                                                                                                >  2:59533/1‑251 (MQ=255)                                  accgaataaaaGATCCTGACCTCGCCGCTGCCGCACGCCGCAGCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGcc                                                                                                                                                                                           <  2:285127/243‑1 (MQ=255)                                          aaaGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACaa                                                                                                                                                                                      >  2:23425/1‑248 (MQ=255)                                            aGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACt                                                                                                                                                                                 >  2:130205/1‑251 (MQ=255)                                                   gAACGCGCCGCAGCCGCACGCCGCAACGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGAGGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCGGCCACGCATTGAGCAAATTTTTGTGAACTGCCCGGCAGGCTGGCGTCTACGCTAGATGTAGCTCCGTCTTTTTATCGCCCGCCGCCGCTTTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTAc                                                                                                                                                                            <  2:50766/249‑1 (MQ=255)                                                               gCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGTGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAATGCGTGCTGGGTTAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACTCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCTTTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTc                                                                                                                                                              <  1:389344/251‑1 (MQ=255)                                                                      gccgcATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTg                                                                                                                                                       >  1:359547/1‑251 (MQ=255)                                                                        cgcATCGTTGAAGAGGAACTGCAACGCGAAACCTTGTCGATTGTGGGCGGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCTTCGCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCTCATTGAAAAGCGTCTCGATGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGt                                                                                                                                                     <  2:175697/251‑1 (MQ=255)                                                                         gcATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTg                                                                                                                                                    >  1:25320/1‑251 (MQ=255)                                                                            tCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAAc                                                                                                                                                 >  2:147869/1‑251 (MQ=255)                                                                                 gaagaagaaCTGCAACTCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAGGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCt                                                                                                                                             <  1:300469/250‑1 (MQ=255)                                                                                 gaagaagaaCTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCt                                                                                                                                             >  1:303304/1‑250 (MQ=255)                                                                                   agaagaaCTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCACACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTACTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCtata                                                                                                                                          <  2:176746/251‑1 (MQ=255)                                                                                        aaCTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGc                                                                                                                                                                                                      <  2:267239/186‑1 (MQ=255)                                                                                        aaCTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGc                                                                                                                                                                                                      >  1:267239/1‑186 (MQ=255)                                                                                         aCTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAGAGGTc                                                                                                                                    >  2:42592/1‑251 (MQ=255)                                                                                              aaCGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTgtgt                                                                                                                               >  1:208738/1‑251 (MQ=255)                                                                                              aaCGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCAGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTCTAAAGGTCtgtgc                                                                                                                               >  1:256764/1‑250 (MQ=255)                                                                                                 gcgAACCCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGTCAGGCTGGCGTCTACGCTATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAATGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATg                                                                                                                            <  1:370763/251‑1 (MQ=255)                                                                                                   gAAACTTTCTCGATTGTGGGGTGGCGTGATGTCCCCACTAACGATATCGTGCTGGGCGAACTCGCCCTCTACTCTCTGAAACGCCTTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGACTGTTTATCGCTCTACGCCGCATTGAAAAACGTCTCGAAGACGACACAGACTTCTAGGTATGTAGCCTGTCGCAGCTGATTACCATCTATAAAAGTATGTGGATGcc                                                                                                                          >  2:31235/1‑251 (MQ=255)                                                                                                      aCCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGAc                                                                                                                       >  1:145874/1‑251 (MQ=255)                                                                                                      aCCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTATGTAGCCTGTCGAATCTGGTGAAAATCTATAAAGGTCTGTGTATGCCgcc                                                                                                                       >  2:231424/1‑249 (MQ=255)                                                                                                       ccTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGACg                                                                                                                      <  1:212674/251‑1 (MQ=255)                                                                                                        cTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCGGTGTATGACGNCgg                                                                                                                     >  2:363038/1‑251 (MQ=255)                                                                                                                         ggCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGACGGATCTGCCGCGCTTTTAt                                                                                                    >  1:182903/1‑251 (MQ=255)                                                                                                                              gATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTgg                                                                                                <  1:94947/250‑1 (MQ=255)                                                                                                                                        cTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGAc                                                                                     >  2:382344/1‑251 (MQ=255)                                                                                                                                                      cTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATc                                                                       >  2:371363/1‑251 (MQ=255)                                                                                                                                                       tGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAACGGTCTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGAGGACCTGCGTCTGGAATCg                                                                      >  1:292895/1‑251 (MQ=255)                                                                                                                                                                tCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGc                                                             >  1:311328/1‑251 (MQ=255)                                                                                                                                                                  gCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCt                                                           <  2:25320/251‑1 (MQ=255)                                                                                                                                                                       ctcctctcTGCCACGCATTGCGCAAATTTTTGTGAACACCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGACGGATCTGCAGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTcc                                                      >  2:336330/1‑251 (MQ=255)                                                                                                                                                                             tctGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGCCCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAgc                                                >  1:2083/1‑251 (MQ=255)                                                                                                                                                                                   aCGCATTGAGCAAATTTTTGTGAACGCGCCGGCAGGCTAGCGTCTACGAGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAATGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAATGGTCTGTGTATGCCGACGGAAATGCCGCGCTTTTATCTGGCTCTTGCGGACCTGCGTCTGGAATCGTCCATTTGCCTGATCCACCAGCGCTtct                                          >  2:90847/1‑251 (MQ=255)                                                                                                                                                                                   aCGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAAAGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGAGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCCTTTGCCTGTTCCACCAGCGCTtct                                          >  1:55881/1‑251 (MQ=255)                                                                                                                                                                                    cGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTtctc                                         >  2:307748/1‑251 (MQ=255)                                                                                                                                                                                                    ttGTGAACGCCCCGGCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTAcc                          <  1:130205/250‑1 (MQ=255)                                                                                                                                                                                                        gAACGCCCCGGCAGGCGGGCGTCTACGCGATATGGAGCGCCGTCGGTTTATCTCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAAGCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGcgct                     <  2:182964/251‑1 (MQ=255)                                                                                                                                                                                                              cccGGCAGGCTGGCGTCTACGCGCTATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCAATGAAAAGCGTCTCGACGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCATCTGGAATCGCCCATTTGCCTGTTCCACCAGCTCTTCTCCACTCACACCggtcctagctgaccgc               >  2:195801/1‑236 (MQ=255)                                                                                                                                                                                                                 ggCAGGCTGGCGTCTACGCGATATGGCACGTCGACTGCTTATCGCCTGCAGCAGATTTGCAAAGAGTCTCGTAGCAGAAAAAGACATCTACGTCCGTTGCCTGCCAAATCTGGTGAACATCTACACAGGACTGTCAATCACGAAGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACTTGCGTCTGGAATCGCCCATTTGCCTGTTCCACCATCGCTTCTCCACCAACACAGTCCCGcgctggccgctgg            >  2:153788/1‑251 (MQ=255)                                                                                                                                                                                                                 ggCAGGCTGGCGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGcgctggccgctgg            >  2:52132/1‑251 (MQ=255)                                                                                                                                                                                                                           cGTCTACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGtt  <  2:208738/251‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       GTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTGTGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTT  >  NC_000913/3354951‑3355419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑