Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,247,458	C→T	intergenic (+30/‑210)	exuR → / → yqiM	DNA‑binding transcriptional repressor ExuR/protein YqiM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,247,458	0	C	T	100.0%	89.9 / NA	29	intergenic (+30/‑210)	exuR/yqiM	DNA‑binding transcriptional repressor ExuR/protein YqiM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/19);  total (10/19)

CGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAA  >  NC_000913/3247212‑3247692                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           cGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGt                                                                                                                                                                                                                                        >  1:356051/1‑251 (MQ=255)  gTCGATAACTTGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGtt                                                                                                                                                                                                                                       <  1:76955/251‑1 (MQ=255)                             cAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGtata                                                                                                                                                                                                             >  2:220907/1‑250 (MQ=255)                              aaatctgcAAGGCGCTGAGTCGCAAAGAGCCTCATGCTGCTAAGCTGGCAATGTGGCAGCAGCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCTAGTTCAATGCCGACCGCTATCTATTCTCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGTCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATaaa                                                                                                                                                                                                           <  2:9457/244‑1 (MQ=255)                                     cAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGCCGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAATGTGTc                                                                                                                                                                                                      >  2:177134/1‑249 (MQ=255)                                     cAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAGGCGAAAAGTAGAAAGTGTc                                                                                                                                                                                                      >  2:244738/1‑249 (MQ=255)                                           gcTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCtgtg                                                                                                                                                                                              <  1:163821/251‑1 (MQ=255)                                              gATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGGATCTCGATACAGCCACCATCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTTTCAGCCTgtgt                                                                                                                                                                                             <  2:53954/249‑1 (MQ=255)                                                              cATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCt                                                                                                                                                                           <  1:317824/251‑1 (MQ=255)                                                              cANGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCGCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCAccc                                                                                                                                                                            <  2:356051/250‑1 (MQ=255)                                                                     ctAAGCTGGCAATGTGGCAGCATCTGGCAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGACCCCCTCCCCTGc                                                                                                                                                                    >  2:14476/1‑251 (MQ=255)                                                                       aaGCTGGCAATGTGGCAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCAt                                                                                                                                                                  >  2:146324/1‑251 (MQ=255)                                                                             gCAATGTGGCAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCa                                                                                                                                                            <  1:265189/251‑1 (MQ=255)                                                                                              ggAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTTCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGa                                                                                                                                           <  1:341644/251‑1 (MQ=255)                                                                                                   acacCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAGCCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCTGTAAGCGAAAAGTATAAAGTGTCAGTCGGTGGAAAGCCTCTCGCTCCCGTCCCCTGCATTCAGCATAATCAgtgtcgacgtacatgt                                                                                                                                      <  2:154964/251‑16 (MQ=255)                                                                                                        aaGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAAttt                                                                                                                                 >  1:263119/1‑251 (MQ=255)                                                                                                                      tAACGAAACCAGCGACGACTTCGAGTGCAATGCCGACCGCTATCTATTCGCCGAAATCCCGGTGGTGCATCTCGATACAGCCACCAGCGTCAGTAAATGAAATTCCTGTCCGACAGGTGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATGCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGaacaa                                                                                                                    <  2:263119/250‑1 (MQ=255)                                                                                                                                   gacgacTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGtt                                                                                                      >  1:237015/1‑251 (MQ=255)                                                                                                                                               ttCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTAGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGCCATGTAATTTTGATAACGAACAACGTTTACCTTTGTTACAATTAGATTc                                                                                          >  2:383509/1‑251 (MQ=255)                                                                                                                                                      ccGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCGCCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTGGTTACAATTAGATTCAATTTGa                                                                                   <  2:373641/251‑1 (MQ=255)                                                                                                                                                      ccGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAAATCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGa                                                                                   <  1:146324/251‑1 (MQ=255)                                                                                                                                                       cGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTCATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGa                                                                                   >  2:308439/1‑250 (MQ=255)                                                                                                                                                       cGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGaa                                                                                  <  2:258874/251‑1 (MQ=255)                                                                                                                                                         aCCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAAtt                                                                                <  2:40544/251‑1 (MQ=255)                                                                                                                                                                      cgcagAAAACCCGGTGGTGCATCTCGATACAGCCAACAGCGGCAGTAAATGAAATTCCTGGCCGACATGCGCTGCAATGCGTCTGTTTGTTTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCTCTGCATTCAGCAAAATCAGCCTCCACGGACATGTAATTTTGATAACGACCAACGTTGAACTTTGTTACAATTAGATTCAACTTGAATTTATGTTTTTGaac                                                                   <  2:60529/247‑2 (MQ=255)                                                                                                                                                                                                        accaGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATGTGAATTTATGTTTTTGAATGCTTTCTTATCTCCCGATTTAACAGGGAATAGtt                                 <  2:331372/251‑1 (MQ=255)                                                                                                                                                                                                                    aaaTGAAATTCCTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTg                     <  1:14476/251‑1 (MQ=255)                                                                                                                                                                                                                              ccTGTCCGACAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATCAAGGGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCaaa           <  1:383509/251‑1 (MQ=255)                                                                                                                                                                                                                                       cAGGCGCTGCAATGCGTCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGaa  <  2:72124/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           CGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTTATCTCACGATTTAACAGGGAATAGTTCAGGCTGTGTTGATGTATCAAACCCGCAGAA  >  NC_000913/3247212‑3247692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑