Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,199,005	G→A	P213S (CCA→TCA)	glnE ←	fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,199,005	0	G	A	100.0%	182.9 / NA	51	P213S (CCA→TCA)	glnE	fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/25);  total (26/25)

GACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGGTTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTCCCCACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGC  >  NC_000913/3198758‑3199243                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               gACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCgttgattg                                                                                                                                                                                                                                             >  1:393939/1‑251 (MQ=255)   cTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCAACAGCGGGCCACTTTCGCCAAACGGACGCAGCAGCATATCCACGCGATAGACGCAGACATCCTGCGTtgattgat                                                                                                                                                                                                                                           >  1:389122/1‑251 (MQ=255)    tGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGCCGAAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATc                                                                                                                                                                                                                                          >  2:191016/1‑251 (MQ=255)     gAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAAGCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATcc                                                                                                                                                                                                                                         <  1:182732/251‑1 (MQ=255)             cTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACttt                                                                                                                                                                                                                                 >  1:340678/1‑251 (MQ=255)                    cGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGc                                                                                                                                                                                                                          >  1:301182/1‑251 (MQ=255)                      aTGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCg                                                                                                                                                                                                                        >  1:271404/1‑251 (MQ=255)                        gTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCt                                                                                                                                                                                                                      >  1:207098/1‑251 (MQ=255)                             gacgGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGcccc                                                                                                                                                                                                                 >  1:263703/1‑251 (MQ=255)                             gacgGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGcccc                                                                                                                                                                                                                 <  2:149944/251‑1 (MQ=255)                                 gAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGc                                                                                                                                                                                                             <  1:334941/251‑1 (MQ=255)                                          cGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTaaaa                                                                                                                                                                                                     >  1:23171/1‑250 (MQ=255)                                                   caTCGCACGCAACACGTTTGCATAGACGCCTTCGCTATCGCCCATCATCCGCGCCTTGACCCTCGCGTACCGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCAACTTTCGCCAACCGGACGCACCCGCATATCCACGCCATAGACGAAGCCATACTGCGTCGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGcgcgc                                                                                                                                                                                           >  2:66211/1‑250 (MQ=255)                                                            caACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGtt                                                                                                                                                                                  <  2:393939/251‑1 (MQ=255)                                                             aaCTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCTTTGATTGATCCAGGACTTTAATCAGCCGCTGCCCCATGCGGTTAAAAAACTGCGCTTTATCCAGTTc                                                                                                                                                                                 <  2:24702/251‑1 (MQ=255)                                                                tCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCg                                                                                                                                                                              >  2:78475/1‑251 (MQ=255)                                                                tCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCg                                                                                                                                                                              >  2:372563/1‑251 (MQ=255)                                                                 cGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCgc                                                                                                                                                                             >  1:223971/1‑250 (MQ=255)                                                                  gTTAGCATAGACGCCTTCGCCATCGCCCACAATCCGAGCCCTGACCATAGCGGACCGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGc                                                                                                                                                                            <  1:109417/251‑1 (MQ=255)                                                                     aGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGt                                                                                                                                                                          <  1:112255/250‑1 (MQ=255)                                                                                cTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCCAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGt                                                                                                                                                               >  2:179084/1‑250 (MQ=255)                                                                                 ttCGCTATCGCCCATAATCCGCGACTTGACCATCGCGTCACGCTCCCCGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGCACGCAGCCGCATATCCACACGATAGACGAAGCCCTCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTCTCCAGTTCCCGGCGTCCACCCTGCGTAc                                                                                                                                                             >  2:155618/1‑251 (MQ=255)                                                                                      tATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAAcc                                                                                                                                                         >  2:310893/1‑250 (MQ=255)                                                                                       aTCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCa                                                                                                                                                        <  1:84445/250‑1 (MQ=255)                                                                                         cGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGt                                                                                                                                                     >  1:243651/1‑251 (MQ=255)                                                                                            ccATAATCCGCGCGTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCACCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTcc                                                                                                                                                  >  2:62906/1‑251 (MQ=255)                                                                                             cATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCg                                                                                                                                                 <  1:29435/251‑1 (MQ=255)                                                                                                  tCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACAATGTTCCGGcc                                                                                                                                              >  1:165585/1‑249 (MQ=255)                                                                                                     gcgcCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCa                                                                                                                                         <  1:314621/251‑1 (MQ=255)                                                                                                       gcCTGGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGTCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCaaa                                                                                                                                       <  1:6516/251‑1 (MQ=255)                                                                                                                   gcgTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGat                                                                                                                           <  2:86707/251‑1 (MQ=255)                                                                                                                       aaCGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATc                                                                                                                        >  1:307239/1‑250 (MQ=255)                                                                                                                        aCGCTCCCATTCGCGCCCCTGCTCCTGGTAATAATCTGCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGCtgattgat                                                                                                                                                                                                                                           <  2:19040/134‑1 (MQ=255)                                                                                                                        aCGCTCCCAGTCGCGCCCCTGCTCCTGGCAATAATCTTCCCACGCGGCAAAGTTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATCGACGAAACAATCCTGCgttgattgct                                                                                                                                                                                                                                           >  1:19040/1‑132 (MQ=255)                                                                                                                               cAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGaa                                                                                                               <  1:354561/251‑1 (MQ=255)                                                                                                                                 gTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAAt                                                                                                             <  1:310893/251‑1 (MQ=255)                                                                                                                                    gcgcCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCTCTGCGCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCa                                                                                                          <  2:247129/251‑1 (MQ=255)                                                                                                                                        cccTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTc                                                                                                      <  1:262808/251‑1 (MQ=255)                                                                                                                                            gCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTCCCCa                                                                                                  <  1:48837/251‑1 (MQ=255)                                                                                                                                                            ttCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCAGCATATCCACGAGATAGCCGAAGCCATCCTGCGTTGATTGATCCAGCCCTTTAATCAGCCGCTGCCCCATGCGGGTAAACAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCCGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTCCCCACCGCCCAGCTTAcccc                                                                                  >  1:362179/1‑250 (MQ=255)                                                                                                                                                                aaCGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTCCCCTCCGCCCAGCTTACCCATGcc                                                                              <  1:114108/251‑1 (MQ=255)                                                                                                                                                                   gcgGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCCAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAACTTAAGCTCCCCACCGCCCAGCTTAACCATGCCTa                                                                            >  2:263884/1‑250 (MQ=255)                                                                                                                                                                     ggCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTCCCCACCGCCCAGCTTACCCATGCCTnaa                                                                          >  2:358584/1‑250 (MQ=255)                                                                                                                                                                        aaaGCTCAGCACCAGCGTGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCCCTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTCCCCACCGCCCAGCTTACCCATGCCTAAAATca                                                                      <  2:23171/251‑1 (MQ=255)                                                                                                                                                                              cAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTCCCCACCGCCCAGCTTACCCATGCCTAAAATCagcagc                                                                 <  1:78475/250‑1 (MQ=255)                                                                                                                                                                              cAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTCCCCACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCg                                                                >  1:64146/1‑251 (MQ=255)                                                                                                                                                                               aGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTCCCCACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCgg                                                               >  2:140930/1‑251 (MQ=255)                                                                                                                                                                                    cAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTCCCCACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTgcg                                                          <  2:3048/251‑1 (MQ=255)                                                                                                                                                                                                    ccAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTCCCCACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCAtt                                          <  1:382059/251‑1 (MQ=255)                                                                                                                                                                                                                agcagCATATCCACGCGATAGACGAAGACATCCTGCGTTGATTAATCCAGCACTTTAATGAGCCGCTGCCCCATGCGGGCAAAAAACTGCGCGTTATCCAGTTCACGGCGTCCACCTTGCGTACAACCATGTTCAGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTACCCACCGCCCAGCTTAACAATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGtccc                              >  2:125334/5‑248 (MQ=255)                                                                                                                                                                                                                     cATATCCACGCGATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTCCCCACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACt                         >  1:103797/1‑251 (MQ=255)                                                                                                                                                                                                                              gcgATAGACGAAGCCATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGACAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTCCCCACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGgcagc                <  1:60295/251‑1 (MQ=255)                                                                                                                                                                                                                                         agccagccTGCGTTGATTGATCCAGCACTGTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTGCCCGGCGTACACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTCCCCACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATAc     <  2:207784/251‑1 (MQ=255)                                                                                                                                                                                                                                            cATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTCCCCACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGc  <  2:271404/251‑1 (MQ=255)                                                                                                                                                                                                                                            cATCCTGCGTTGATTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTCCCCACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGc  >  1:15502/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               GACTGAATCACGCTGAAATCGATGTAACGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGGTTGATCCAGCACTTTAATCAGCCGCTGCCCCATGCGGGTAAAAAACTGCGCGTTATCCAGTTCCCGGCGTCCACCCTGCGTACAACCATGTTCCGGCCAGGCAAAAATCAGATCGATATCAGAGGAGAAATTCAGCTCCCCACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGC  >  NC_000913/3198758‑3199243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑