Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,342,582	C→T	D530N (GAT→AAT)	yfaL ←	putative autotransporter adhesin YfaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,342,582	0	C	T	100.0%	230.4 / NA	68	D530N (GAT→AAT)	yfaL	putative autotransporter adhesin YfaL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/31);  total (37/31)

CTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAGCACCGCACCATCATCAACGACTATATCGCCGGTTAGCGCCATCGACTGCGCCCCTGCCAGCACGTAGCTGC  >  NC_000913/2342343‑2342827                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      ccccATCACTCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCTGTCGTCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCg                                                                                                                                                                                                                                             <  1:168950/248‑1 (MQ=255)   ccATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATCGCGGGTATCTCCCAGCTGCGAGTTGTATCTCCCCATTCGCGTACCCAAGGCGCTTTGTTGTGCGCCCAAATAACTTTTACTCTTTGCCCGACTTACCTGCCCGTCGCTCGCATCCGCCACCACGTTCACCCCCTCTTTCCCTTCGCCGCCCATATGCACATTGTCCCCACACG‑CACGCTCTTCCCCATTCTGACTGcccatt                                                                                                                                                                                                                                          >  2:232230/1‑247 (MQ=255)   ccATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGa                                                                                                                                                                                                                                            <  1:353546/249‑1 (MQ=255)       cACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTT‑CAAGATCTACCACATTCTGACTGCCGATAAccc                                                                                                                                                                                                                                      >  1:106052/1‑250 (MQ=255)         cGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGtt                                                                                                                                                                                                                                    <  1:399095/251‑1 (MQ=255)             ttCTTGTTGCTTATCGGTAAGGAGAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGGGAGTTGTCGCTCCCAATCAGCGTACCGGGGGCGATTGGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCCAGCATCGACCACCAAGTACAAGCCATCTTTCCCGTGGCCGCCGATATGCAAATTGTCGGCCCCTG‑CAAGATCTACCACAGTCGGACTGCCGATAACCGTTCCg                                                                                                                                                                                                                                 <  2:168900/250‑1 (MQ=255)                     gCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCa                                                                                                                                                                                                                        <  1:276764/251‑1 (MQ=255)                                 aGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCCACGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCCGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCCAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGATGCTGCCACTGACTTCAAgg                                                                                                                                                                                                            >  1:175062/1‑251 (MQ=255)                                    tAACCTGGCGGTTATAGGGGGTATCTCCCAGCTGCGAGTTGTCGCTCCCCCTCAGCGTACCCGAGGCGGATTGTGTTGTGCCCCAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCCCGCATCGACCACCACGTAGACGCCATCTTTCCCCTCGCCGCCGCTATGCCAATTGTCGCCACCCG‑CAAAATCTACCACATTCTGAATGCCGATAACCGTTCCGCTGCCGCCACTGACTTCCAGGcac                                                                                                                                                                                                         >  2:153967/1‑249 (MQ=255)                                               ttATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTatg                                                                                                                                                                                               >  2:234992/1‑250 (MQ=255)                                                        gTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTCCAGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGGCTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCCAGGCCATCGTTGTATGCTGTGCCGct                                                                                                                                                                                     >  2:57319/1‑251 (MQ=255)                                                        gTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGct                                                                                                                                                                                     >  2:292501/1‑251 (MQ=255)                                                              ccAATCTGCGAGTTGCCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCCCCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTg                                                                                                                                                                                  <  2:326647/248‑1 (MQ=255)                                                              ccAAGCTGCGATTTGTCGCTCACCACCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCTGACTTCCCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTCCCGGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCa                                                                                                                                                                               <  2:136535/251‑1 (MQ=255)                                                              ccAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTT‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTg                                                                                                                                                                                  <  1:23725/248‑1 (MQ=255)                                                              ccAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGcc                                                                                                                                                                                >  2:281974/1‑250 (MQ=255)                                                                    ttcGAGTTGCCGCTCACAACCAGCGTCACGGAGGCGCCTTGAGTTGTGCCCAAATTACTATTATTGTTGGCCAGACTTACCTCCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTACCGTCGCCGCCGATATGCACATTGTCGCCACCTG‑CAAGATCTACCACACTCTGACTGCCGATAACCGTTCCGATGGTGCCACTGACTACAAGACCATCGTTGTATGCTGTGCCGCACTGCCAGGTggg                                                                                                                                                                         >  2:251172/3‑250 (MQ=255)                                                                     gCGAGTTGTCGCTCACCATCAGCGTACCGCAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTCCCTGCCCGTCGCTCGCATCGACCACCACGTATACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTTCCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGga                                                                                                                                                                         <  1:273832/250‑1 (MQ=255)                                                                       gAGTTGTCGCTCACCATCATCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGaa                                                                                                                                                                      <  1:366877/251‑1 (MQ=255)                                                                               gCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCagaga                                                                                                                                                              <  1:293177/251‑1 (MQ=255)                                                                                    ccagcAGCGGACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCGCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCg                                                                                                                                                         <  1:163737/247‑1 (MQ=255)                                                                                         aGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGcac                                                                                                                                                    >  1:210591/1‑251 (MQ=255)                                                                                                 ggAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGCTGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGt                                                                                                                                            >  2:38359/1‑251 (MQ=255)                                                                                                   aGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACTTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGttt                                                                                                                                          <  1:154554/251‑1 (MQ=255)                                                                                                      cGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATATTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAac                                                                                                                                       >  1:348710/1‑251 (MQ=255)                                                                                                        aTTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAacac                                                                                                                                     >  2:305281/1‑251 (MQ=255)                                                                                                          tttggtgtggCCCAAATAACTATTATTGTTTGCCAAACTTACCTGCCCGTCGCTCGCATCGCCCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGATCACACCGCCGTTTAACACg                                                                                                                                    <  2:61295/241‑1 (MQ=255)                                                                                                           tgtgttgtgCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGa                                                                                                                                   <  1:305281/250‑1 (MQ=255)                                                                                                           tgtgttgtgCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGAt                                                                                                                                  >  2:245281/1‑251 (MQ=255)                                                                                                                  tgCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGc                                                                                                                           >  2:82437/1‑251 (MQ=255)                                                                                                                    cccAACTAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCAc                                                                                                                                                                                                                       >  1:78919/1‑157 (MQ=255)                                                                                                                    cccAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCAc                                                                                                                                                                                                                       <  2:78919/157‑1 (MQ=255)                                                                                                                       aaaTAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATc                                                                                                                      >  1:205469/1‑251 (MQ=255)                                                                                                                            aCTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCt                                                                                                                 <  1:137581/251‑1 (MQ=255)                                                                                                                                     gTTTGCCAGACTGCCCTGCCCGTCGCTCGCACCGACCACCCCGTAGACCCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCgg                                                                                                        <  1:11769/251‑1 (MQ=255)                                                                                                                                        tcccagcctcaCCTGCCAGTCTCCCGCCTCGACCCACACGGACACGCCCTCTTTACCGTCCCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGAGCTACCACATTCTGCCTGCCGATAACCTTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAACTCAGAGAGATCGACCTCACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGTGGTa                                                                                                      <  2:152346/240‑1 (MQ=255)                                                                                                                                        tGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTa                                                                                                      >  2:111463/1‑250 (MQ=255)                                                                                                                                         gCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTa                                                                                                      >  2:234573/1‑249 (MQ=255)                                                                                                                                          ccAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATATTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CACGATCTACCACCTTCTGACTGCCTATAACCGTTCCGCTGCTGCCACTGCCTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCTCCGTTTAACACGATCGACTGCGGATCGTCATGGAGATCggga                                                                                                      >  2:328350/1‑246 (MQ=255)                                                                                                                                            aGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGACATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTa                                                                                                      >  2:141837/1‑246 (MQ=255)                                                                                                                                                 tACCGGCCCGTCGCTCGCATCGACCACCACGTAGACTCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGc                                                                                              <  2:210591/249‑1 (MQ=255)                                                                                                                                                    cTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CACGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGt                                                                                         >  2:153659/1‑251 (MQ=255)                                                                                                                                                       cccGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGACGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGc                                                                                      >  2:336421/1‑251 (MQ=255)                                                                                                                                                             gctcgcATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTcc                                                                                 >  1:65318/1‑250 (MQ=255)                                                                                                                                                                  cATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGa                                                                            >  2:214532/1‑250 (MQ=255)                                                                                                                                                                     cGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAgc                                                                        <  1:38359/251‑1 (MQ=255)                                                                                                                                                                      gACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAgc                                                                        >  2:123389/1‑250 (MQ=255)                                                                                                                                                                      cacccacatgTAGACGACATGTTTCCCGTGGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCGGACTGCCGGTAACCGTTCCGCTGCTGCCACTGACTGCAAGGCCATCGTTGTATGAAGTGCCGCACTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACGGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAgca                                                                       <  1:45843/242‑1 (MQ=255)                                                                                                                                                                        ccaccaCGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCAGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAgcacc                                                                     >  2:282614/1‑251 (MQ=255)                                                                                                                                                                            cacGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAgcaccgcac                                                                 >  1:387424/1‑251 (MQ=255)                                                                                                                                                                            cacGTAGACGCCATCTTTCCCGTCGCCCACGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGATCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCGTTCCAGCGAAAgcaccgcac                                                                 >  1:288304/1‑251 (MQ=255)                                                                                                                                                                                            tccctgTCGCCGCCGAAATGCAAATCGTCGCC‑GCTGTCAAGATCTCCCACATTTTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTGCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTTGAGAAATCAGAGAGATCGAGCACACCGCCGTGTAACACGATCGACTGCGGATCGTCCTGGAGAGCTGTAAGATCTGCCGCTTCGCCTTCCATCGAAAGCACCGCACCCTCATCAACGACta                                                  <  2:28502/245‑1 (MQ=255)                                                                                                                                                                                                cGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAGCACCGCACCATCATCAACGACTATATCg                                             <  1:232637/251‑1 (MQ=255)                                                                                                                                                                                                cGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCACTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATAGACTGCGGATCGGCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAGCACCACACCATAATCACCGACTATATcc                                             >  2:195853/1‑250 (MQ=255)                                                                                                                                                                                                   cgccgcCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAGCACCGCACCATCATCAACGACTATATCGCCg                                          <  1:234992/251‑1 (MQ=255)                                                                                                                                                                                                   cgccgcCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAGCACCGCACCATCATCAACGACTATATCGCCg                                          <  1:281974/251‑1 (MQ=255)                                                                                                                                                                                                             ctgccacttctcggcACCTG‑AAAGATCTACCACATTCTGACTGACGATAACCGTTCCGCTGCTGCCATTGACTTCAAGGCCATCGTTGTATGATGAGCCGCTCTGCAAGGGGGAGAAATCAGAGACATCGAGCACACCGCCGCTTAACACGACCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCAGCGAAGCCTTCCAGCGAAAGCACCGCACTATCATCAACGTATATATCGCCGGATAgcnac                                 >  2:354468/15‑247 (MQ=255)                                                                                                                                                                                                             atGCAAATTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAGCACCGCACCATCATCAACGACTATATCGCCGGTTAGCGcc                                 >  1:328051/1‑250 (MQ=255)                                                                                                                                                                                                                 aaaTTGTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAGCACCGCACCATCATCAACGACTATATCGCCGGTTAGCGCCATCGa                            <  1:107996/251‑1 (MQ=255)                                                                                                                                                                                                                      gTCGCCACCTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAGCACCGCACCATCATCAACGACTATATCGCCGGTTAGCGCCATCGACTgc                        <  1:153967/250‑1 (MQ=255)                                                                                                                                                                                                                           cgccTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAGCACCGCACCATCATCAACGACTATATCGCCGGTTAGCGCCATCGACTGCGCCCCt                  <  1:180541/249‑1 (MQ=255)                                                                                                                                                                                                                             ccTG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAGCACCGCACCATCATCAACGACTATATCGCCGGTTAGCGCCATCGACTGCGCCCCTGc                >  1:71744/1‑251 (MQ=255)                                                                                                                                                                                                                               tG‑CAAGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAGCACCGCACCATCATCAACGACTATATCGCCGGTTAGCGCCATCGACTGCGCCCCTGcc               >  1:186118/1‑250 (MQ=255)                                                                                                                                                                                                                                   aaGATCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCCGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAGCACCGCACCATCATCAACGACTATATCGCCGGTTAGCGCCATCGACTGCGCCCCTGCCAGCa           <  2:155275/251‑1 (MQ=255)                                                                                                                                                                                                                                     gATCTACCAAATTCTGCCTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCCGCGAAAGCACCGCACCATCATCAACGACTATATCGCCGGTTCGCGCCATCGACTGCGCCCCTGCCAGCACg         >  2:284542/1‑251 (MQ=255)                                                                                                                                                                                                                                      aTCTACCACATTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAGCACCGCACCATCATCAACGACTATATCGCCGGTTAGCGCCATCGACTGCGCCCCTGCCAGCACGt        >  2:108095/1‑251 (MQ=255)                                                                                                                                                                                                                                          tccACATTCTGACTTCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCTTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAGCACCGCACCATCATCAACGACGATATCGCCGGTTAGTGCCATCGACTGCGCCCCTGCCAGCACGTa       <  1:117668/247‑1 (MQ=255)                                                                                                                                                                                                                                            cacaTTCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAGCACCGCACCATCATCAACGACTATATCGCCGGTTAGCGCCATCGACTGCGCCCCTGCCAGCACGTAGCTGc  >  1:372344/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      CTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTG‑CAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATCGAGCACACCGCCGTTTAACACGATCGACTGCGGATCGTCCTGGAGAGCGGTAAGATCTGCCGCGTCGCCTTCCAGCGAAAGCACCGCACCATCATCAACGACTATATCGCCGGTTAGCGCCATCGACTGCGCCCCTGCCAGCACGTAGCTGC  >  NC_000913/2342343‑2342827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑