Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	62.9 / NA	18	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/9);  total (9/9)

GGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGG‑AACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATT‑AACGAGACTGAGCAAAGAGCATATCAT‑TGATGTTC‑ACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTAT  >  NC_000913/1652109‑1652554                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                ggAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGATGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGTCCGTTTCACGGTACCAAAAACACAGGCGGG‑AACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATT‑AACGAGACTGAGCAAAGACCATATCAG‑TGATGTTC‑ACCTCAGAGAGTATGGCTGAACAGAAAAAc                                                                                                                                                                                                      <  2:42328/250‑1 (MQ=255)      tCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGG‑AACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATT‑AACGAGACTGAGCAAAGAGCATATCAT‑TGATGTTC‑ACCTCCGAGAGTATGGcag                                                                                                                                                                                                                 >  1:309374/1‑234 (MQ=255)      tCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGG‑AACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATT‑AACGAGACTGAGCAAAGAGCATATCAT‑TGATGTTC‑ACCTCAGAGAGTATGGcag                                                                                                                                                                                                                 <  2:309374/234‑1 (MQ=255)                  gCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGG‑AACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATT‑AACGAGACTGAGCAAAGAGCATATCAT‑TGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGttt                                                                                                                                                                                    >  1:177348/1‑251 (MQ=255)                     gtgtgCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGG‑AACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATT‑AACGAGACTGAGCAAAGAGCATATCAT‑TGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGttttt                                                                                                                                                                                  <  2:177348/250‑1 (MQ=255)                                               aTCTGAAAAATGGAACAATGATGATCAGGAGAATTTTAGCAAAAGACGGTTTAACGGTACCAAAAACACAGGCGGG‑AACCAAACGGGTCCTTCATTTTTTTAAGCCCGAAATCGAGCCTCTCCGGAGTAAGAGGACATTTAAC‑AGACTGGGCAAAAATCATAT‑ATATGATTTTCCACC‑CAGAGAGTAAGGCAGAACAGAAAAAAAAAAATGCAACTTTGTTTTTTAACCTAAAGAGTCAACGAGAGTaaa                                                                                                                                                        >  2:201012/1‑250 (MQ=255)                                                                                       aaaaGACCGTTTCACGGTACCAAAAACACAGGCGGGGAACCA‑TCGGGGCATTCATCTTATTAAACCAACAATCGACGATATCCGGAGATAGATGACATT‑AACGGGACTGAGCAAAAAGGATATCAT‑TGATTTTC‑ACCCCAGAGAGTATGGCAGAACAAGAAAACAAAAATGCACCTGTGTTTTTCAACCTGAAAGGTCAGCGGAAGTAAAAAATTAGGGTTACCATTTTaacggttgctaaaaaaggg                                                                                                                  >  1:288443/1‑230 (MQ=255)                                                                                                    aCGGTACCAAAAACACAGGCGGG‑GAACAATTGGGTCATTTATTTTTTTAAGCCAGCAATTGACGCTCTCCGGAGTCAGATGACATT‑AACGAGACTGAGCAAAGAGCAAATCAT‑TGATGTTC‑ACCTCAGAGAGTATGGCAGAAAAGAAAAACAAAAAATCACCTTTTTTTTTTAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAACATTTTATCGTTTACTCAATAAGGGAAATGTGGGCCGCAGc                                                                                                  >  2:9393/1‑251 (MQ=255)                                                                                                                              ccAATCGGGTCATTCATCTTAGTAAGCCAGCAATCGACGCTCTCCCGAGTCAGATGACATT‑AACGAGACTGAGCAAAGAGCATATCAT‑TGATGTTC‑ACCTCAGAGAGTATGTCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCATCGATAAAACGTGCCGGACTCCGCCAt                                                                         <  1:283679/251‑1 (MQ=255)                                                                                                                                       tcattcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATT‑AACGAGACTGAGCAAAGAGCATATCAT‑TGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATc                                                                 >  2:100738/1‑250 (MQ=255)                                                                                                                                                  attaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATT‑AACGAGACTGAGCAAAGAGCATATCAT‑TGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGAc                                                                                                       <  2:208875/201‑1 (MQ=255)                                                                                                                                                  attaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATT‑AACGAGACTGAGCAAAGAGCATATCAT‑TGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGAc                                                                                                       >  1:208875/1‑201 (MQ=255)                                                                                                                                                          aGCAATCGACGCTCTCCGGAGTCAGATGACATT‑AACGAGACTGAGCAAAGAGCATATCAT‑TGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACtt                                             <  2:164301/251‑1 (MQ=255)                                                                                                                                                                                     gACATT‑AACGAGACTGAGCAAAGATCATATCAT‑TGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTg                  <  1:25237/251‑1 (MQ=255)                                                                                                                                                                                               gagaCTGAGCAAAGAGCATATCAT‑TGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGa                                                                                                        <  1:289801/156‑1 (MQ=255)                                                                                                                                                                                               gagaCTGAGCAAAGAGCATATCAT‑TGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGa                                                                                                        >  2:289801/1‑156 (MQ=255)                                                                                                                                                                                                agaCTGAGCAAAGAGCATATCAT‑TGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGc        <  1:71087/251‑1 (MQ=255)                                                                                                                                                                                                        cAAAGAGCATATCAT‑TGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTtat  >  1:283674/1‑249 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                GGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGG‑AACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATT‑AACGAGACTGAGCAAAGAGCATATCAT‑TGATGTTC‑ACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTAT  >  NC_000913/1652109‑1652554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑