Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,583,650	G→A	A13V (GCC→GTC)	ydeO ←	DNA‑binding transcriptional dual regulator YdeO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,583,650	0	G	A	100.0%	37.0 / NA	12	A13V (GCC→GTC)	ydeO	DNA‑binding transcriptional dual regulator YdeO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/5);  total (7/5)

ACATTTGAAGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGTGCATTGTAGATATTTTTTAATGGTATTTTCAGTAAATCCGATCTCTTTAAAATGCCGCTCATAAGGTTCAAAATGCGTACGAACAGCGTTATCTACCATCAGGATCTCGCCGTCAGAGAAGGCGTAATCTTTATCTAAAATGTTAGCGTTGAAGGCATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTTGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATA  >  NC_000913/1583437‑1583880                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       aCATTTGAAGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGAGCATTGTAGATATTTTTTAATGGTATTTTCAGTAAATCCGATCTCTTTAAAATGCCGCTCATAAGGTTCAAAATGCGTACGAACAGCGTTATCTACCATCAGGATCTCGCCGTCAGAGAAGGCGTAATCTTTATCTAAAATGTTAGCGTTGAAGACATGATGAATAAATATAACAGAACAAACGAGCGACAt                                                                                                                                                                                                   >  2:63728/1‑251 (MQ=255)  cATTTGAAGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGTGCATTGTAGATATTTTTTAATGGTATTTTCAGTAAATCCGATCTCTTTAAAATGCCGCTCATAAGGTTCAAAATGCGTACGAACAGCGTTATCTACCATCAGGATCTCGCCGTCAGAGAAGGCGTAATCTTTATCTAAAATGTTAGCGTTGAAGACATGATGAATAAATATAACAGAACAAACGTGCGACAtt                                                                                                                                                                                                  <  1:75273/251‑1 (MQ=255)             cGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGTGCATTGTAGATATTTTTTAATGGTATTTTCAGTAAATCCGATCTCTTTAAAATGCCGCTCATAAGGTTCAAAATGCGTACGAACAGCGTTATCTACCATCAGGATCTCGCCGTCAGAGAAGGCGTAATCTTTATCTAAAATGTTAGCGTTGAAGACATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTa                                                                                                                                                                                       <  1:61983/251‑1 (MQ=255)                                  ggCACCGTCACTGTCTGGATGTTAGTGCATTGTAGATATTTTTTAATGGTATTTTCAGTAAATCCGATCTCTTTAAAATGCCGCTCATAAGGTTCAAAATGCGTACGAACAGCGTTATCTACCATCAGGATCTCGCCGTCAGAGAAGGCGTAATCTTTATCTAAAATGTTAGCGTTGAAGACATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCg                                                                                                                                                                   >  1:164443/1‑250 (MQ=255)                                                                              caTGGTATTTTCAGTAACTCAGATATCTTTAACATGCCGCTCATAAGGTTCAAAATGAGTACGAACAGCGTTATCTACCATCAGGATCTCGCCGTCCGAGAAGGCGTAATCTTTATCTAAAATGTTAGCGTTGAAGACATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACACTAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTc                                                                                                                       >  2:135487/2‑250 (MQ=255)                                                                                         cAGTAAATCCGATCTCTTTAAAATGCCGCTCATAAGGTTCAAAATGCGTACGAACAGCGTTATCTACCATCAGGATCTCGCCGTCAGAGAAGGCGTAATCTTTATCTAAAATGTTAGCGTTGAAGACATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTTGCAAAc                                                                                                           <  2:161875/251‑1 (MQ=255)                                                                                                          ttAAAATGCCGCTCATAAGGTTCAAAATGCGTACGAACAGCGTTATCTACCATCAGGATCTCGCCGTCAGAGAAGGCGTAATCTTTATCTAAAATGTTAGCGTTGAAGACATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTTGCAAACTATTGATAATGaaa                                                                                             <  1:79689/248‑1 (MQ=255)                                                                                                                  ccGCTCATAAGGTTCAAAATGCGTACGAACAGCGTTATCTACCATCAGGATCTCGCCGTCAGAGAAGGCGTAATCTTTATCTAAAATGTTAGCGTTGAAGACATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTTGCAAACTATTGATAATGAAATGTGTa                                                                                       >  2:62537/1‑246 (MQ=255)                                                                                                                  ccGCTCATAAGGTTCAAAATGCGTACGAACAGCGTTATCTACCATCAGGATCTCGCCGTCAGAGAAGGCGTAATCTTTATCTAAAATGTTAGCGTTGAAGACATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTTGCAAACTATTGATAATGAAATGTGTa                                                                                       >  2:6869/1‑246 (MQ=255)                                                                                                                                             aaCAGCGTGATCTACCATCAGGATCTCGCCGTCAGAGAAGGCGTAATCTTTATCTAAAATGATAGAGTTGAAGACATGATGAATAAATATAACAGAACAAACGAGCGAAATTTTATCTCCTTAACACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTTGCAAACTATTGATAATGACATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAAt                                                       >  2:240233/1‑251 (MQ=255)                                                                                                                                                                              agagAATGCGTAATCTTTATCTAAAATTTTAGCGTGGAAGACATGATGAATAAATATAACAGAACAAACGAACGACATTTTATCTCCTTAAAACAATAAAGTTTTTTTCTAGACGTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTTGCAAACTATTGATAATGAAATGTGTAAGATTTCTTGCATTGATACACATTTGTGAAATACTCGGCTGATCAATTGCCGAAAAAATAGCTAt                      <  2:394736/251‑1 (MQ=255)                                                                                                                                                                                                   tAAAATGTTAGCGTTGAAGACATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTTGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGtaata  >  1:17127/1‑250 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       ACATTTGAAGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGTGCATTGTAGATATTTTTTAATGGTATTTTCAGTAAATCCGATCTCTTTAAAATGCCGCTCATAAGGTTCAAAATGCGTACGAACAGCGTTATCTACCATCAGGATCTCGCCGTCAGAGAAGGCGTAATCTTTATCTAAAATGTTAGCGTTGAAGGCATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTTGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATA  >  NC_000913/1583437‑1583880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑