Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,579,410	G→A	intergenic (‑68/‑135)	yddA ← / → yneP	ABC transporter family protein YddA/protein YneP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,579,410	0	G	A	100.0%	62.7 / NA	19	intergenic (‑68/‑135)	yddA/yneP	ABC transporter family protein YddA/protein YneP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/8);  total (11/8)

ATTCCAGTCGTTTAACCAGACCTGAATTTTAACCACGCCAAGGATCATGGCGAGGATAATAATGATTAACAACACTGAAGTTTTATTATTCTTACGCAGCCAAAAGGGCTTCAACAGACAGAGATACTTTGCTATCAACATACGAAGCGTAATGGGAATGGTTATCATTAGCGAAAATTGATTGTGCCATCCAATGATTATCTATGGCAATGATTTACTGATGAAATTTATTTAAGTATCATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATT  >  NC_000913/1579175‑1579648                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               attCCAGTCGTTTAACCAGACCTGAATTTTAACCACGCCAAGGATCATGGCGAGGATAATAATGATTAACAACACTGAAGTTTTATTATTCTTACGCAGCCAAAAGGGCTTCAACAGACAGAGATACTTTGCTATCAACATACGAAGCGTAATGGGAATGGTTATCATTAGCGAAAATTGATTGTGCCATCCAATGATTATCTATGGCAATGATTTACTGATGAAATTTATTTAAATATCATTTAACAata                                                                                                                                                                                                                                 <  2:137824/251‑1 (MQ=255) attCCAGTCGTTTAACCAGACCTGAATTTTAACCACGCCAAGGATCATGGCGAGGATAATAATGATTAACAACACTGAAGTTTTATTATTCTTACGCAGCCAAAAGGGCTTCAACAGACAGAGATACTTTGCTATCAACATACGAAGCGTAATGGGAATGGTTATCATTAGCGAAAATTGATTGTGCCATCCAATGATTATCTATGGCAATGATTTACTGATGAAATTTATTTAAATATCATTTAACAat                                                                                                                                                                                                                                  >  1:368649/1‑250 (MQ=255)    ccAGTCGTTTAACCAGACCTGAATTTTAACCACGCCAAGGATCATGGCGAGGATAATAATGATTAACAACACTGAAGTTTTATTATTCTTACGCAGCCAAAAGGGCTTCAACAGACAGAGATACTTTGCTATCAACATACGAAGCGTAATGGGAATGGTTATCATTAGCGAAAATTGATTGTGCCATCCAATGATTATCTATGGCAATGATTTACTGATGAAATTTATTTAAATATCATTTAACAATATGa                                                                                                                                                                                                                              <  2:262549/251‑1 (MQ=255)     cAGTCGTTTAACCAGACCTGAATTTTAACCACGCCAAGGATCATGGCGAGGATAATAATGATTAACAACACTGAAGTTTTATTATTCTTACGCAGCCAAAAGGGCTTCAACAGACAGAGATACTTTGCTATCAACATACGAAGCGTAATGGGAATGGTTATCATTAGCGAAAATTGATTGTGCCATCCAATGATTATCTATGGCAATGATTTACTGATGAAATTTATTTAAATATCATTTAACAATATGaa                                                                                                                                                                                                                             <  1:401549/251‑1 (MQ=255)                          aTTTTAACCACGCCAAGGATCATGGCGAGGATAATAATGATTAACAACACTGAAGTTTTATTATTCTTACGCAGCCAAAAGGGCTTCAACAGACAGAGATACTTTGCTATCAACATACGAAGCGTAATGGGAATGGTTATCATTAGCGAAAATTGATTGTGCCATCCAATGATTATCTATGGCAATGATTTACTGATGAAATTTATTTAAATATCATTTAACAATATGAAATATAAAGGAATATTTCAttt                                                                                                                                                                                                        >  1:384367/1‑251 (MQ=255)                              tAACCACGCCAAGGATCATGGCGAGGATAATAATGATTAACAACACTGAAGTTTTATTATTCTTACGCAGCCAAAAGGGCTTCAACAGACAGAGATACTTTGCTATCAACATACGAAGCGTAATGGGAATGGTTATCATTAGCGAAAATTGATTGTGCCATCCAATGATTATCTATGGCAATGATTTACTGATGAAATTTATTTAAATATCATTTAACAATATGAAATATAAAGGAATATTTCATTTTCa                                                                                                                                                                                                     <  1:113609/250‑1 (MQ=255)                                                 ggCNAGGATAATAATGATTAACAACACTGAAGTTTTATTATTCTTACGCAGCCAAAAGGGCTTCAACAGACAGAGATACTTTGCTATCAACATACGAAGCGTAATGGGAATGGTTATCATTAGCGAAAATTGATTGTGCCATCCAATGATTATCTATGGCAATGATTTACTGATGAAATTTATTTAAATATCATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTAtt                                                                                                                                                                                 <  2:360751/251‑1 (MQ=255)                                                     aGGATAATAATGATTAACAACACTGAAGTTTTATTATTCTTACGCAGCCAAAAGGGCTTCAACAGACAGAGATACTTTGCTATCAACATACGAAGCGTAATGGGAATGGTTATCATTAGCGAAAATTGATTGTGCCATCCAATGATTATCTATGGCAATGATTTACTGATGAAATTTATTTAAATATCATTTAACAATATGAAATCTAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGAt                                                                                                                                                                              >  2:6995/1‑250 (MQ=255)                                                                                 ttttATTATTCTTACGCAGCCAAAAGGGCTTCAACAGACAGAGATACTTTGCTATCAACATACGAAGCGTAATGGGAATGGTTATCATTAGCGAAAATTGATTGTGCCATCCAATGATTATCTATGGCAATGATTTACTGATGAAATTTATTTAAATATCATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGc                                                                                                                                                 >  2:237777/1‑251 (MQ=255)                                                                                               cGCAGACAAAAGGGCTTCAACAGACAGAGATACTTTGCTATCAACATACGAAGCGTAATGGGAATGGTTATCATTAGCGAAAATTGATTGTGCCATCCAATGATTATCTATGGCAATGATTTACTGATGAAATTTATTTAAATATCATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGcc                                                                                                                                   <  2:225395/251‑1 (MQ=255)                                                                                                                                          aCATACGAAGCGTAATGGGAATGGTTATCATTAGCGAAAATTGATTGTGCCATCCAATGATTATCTATGGCAATGATTTACTGATGAAATTTATTTAAATATCATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGAc                                                                                         >  1:235023/1‑250 (MQ=255)                                                                                                                                                      tAATGGGAATGGTTATCATTAGCGAAAATTGATTGTGCCATACAATGATTATCTATGGCAATGATTTACTGATGAAATTTATTTAAATATCATTTAACAATATGAAATATAAAGGAATATTTCTTTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTc                                                                            >  2:69065/1‑251 (MQ=255)                                                                                                                                                        aTGGGAATGGTTATCATTAGCGAAAATTGATTGAGCCATCCACTGATTATCTATGGCAATGATTTACTGATGAAATTTATTTAAATATCATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATAAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACACGACAGGAATTTATGNCgg                                                                          >  2:362694/1‑251 (MQ=255)                                                                                                                                                                                            cATCCAATGATTATCTATGGCAATGATTTACTGATGAAATTTATTTAAATATCATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAAt                                      <  1:109728/251‑1 (MQ=255)                                                                                                                                                                                               ccAATGATTATCTATGGCAATGATTTACTGATGAAATTTATTTAAATATCATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCa                                                                                                                                  <  1:277453/156‑1 (MQ=255)                                                                                                                                                                                                         tCTATGGCAATGATTTACTGATGAAATTTATTTAAATATCATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGGGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGGGTTAAAag                           >  2:246370/1‑249 (MQ=255)                                                                                                                                                                                                              ggCAATGATTTACTGATGAAATTTATTTAAATATCATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAATAGAGCGt                    >  2:72895/1‑251 (MQ=255)                                                                                                                                                                                                                             atgaAATTTATTTAAATATCATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTcc     >  2:194720/1‑251 (MQ=255)                                                                                                                                                                                                                                aaaTTTATTTAAATATCATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGGCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCAtt  >  1:288112/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               ATTCCAGTCGTTTAACCAGACCTGAATTTTAACCACGCCAAGGATCATGGCGAGGATAATAATGATTAACAACACTGAAGTTTTATTATTCTTACGCAGCCAAAAGGGCTTCAACAGACAGAGATACTTTGCTATCAACATACGAAGCGTAATGGGAATGGTTATCATTAGCGAAAATTGATTGTGCCATCCAATGATTATCTATGGCAATGATTTACTGATGAAATTTATTTAAGTATCATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATT  >  NC_000913/1579175‑1579648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑