Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,567,651	G→A	N391N (AAC→AAT)	digH ←	divisome localized glycosyl hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,567,651	0	G	A	100.0%	54.5 / NA	18	N391N (AAC→AAT)	digH	divisome localized glycosyl hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/13);  total (5/13)

TAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGTCGGTTTAACGACATCCGCCCACCATTTTGCCAACACGTCATAACGCGCGGCACTCCGTGAGAACGGCCAGTAAATTTGGGGAGCAATGTAATCCAGCAATCCTTGTTCCACCCATCGACGGGTGTCAGCGTAGGATTCGTCATAGGCTGCCGCGCCTCGGGTA  >  NC_000913/1567415‑1567894                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    taagtaagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCATTAATCATCCAGTc                                                                                                                                                                                                                                       >  1:166920/1‑251 (MQ=255)  aagtaagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCATTAATCATCCAGTc                                                                                                                                                                                                                                       <  1:263727/250‑1 (MQ=255)  aagtaagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCATTAATCATCCAGTCt                                                                                                                                                                                                                                      <  1:105318/251‑1 (MQ=255)                       ggATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCATTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTc                                                                                                                                                                                                                  >  1:79990/1‑250 (MQ=255)                           aGTTTAAGAGGGTAACAAGCCAGTTGGTAAAGCACCGACTTGTTACAAAGTAAGAATGGGAGTTTAACGGCCCCAGCGACTTTGCAGATAGTTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCGCGGAACACGATGGTTCCGCTAATTCCGGGCCCTGCCTCGTTAAGATCGTGCTGCTTTTTCAGTTCCGCTACTCCGCCATTAATCATCCAGTCGGCCTCTATCTTTGCAGGTTCAcccc                                                                                                                                                                                                             <  2:46504/251‑2 (MQ=255)                                        aaCAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCATTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGc                                                                                                                                                                                                <  1:259932/251‑1 (MQ=255)                                           aaGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCATTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGAt                                                                                                                                                                                             >  2:238213/1‑251 (MQ=255)                                                       aaaGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCATTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGCAGGCGATACCGATATAc                                                                                                                                                                                   >  2:109745/1‑249 (MQ=255)                                                                           aGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCATTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTTTAGAAGGCGATGCCGATATACAGGCGGGTCCTg                                                                                                                                                                       <  1:13611/241‑1 (MQ=255)                                                                           aGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCATTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTTTAGAAGGCGATACCGATATACAGGCGGGtgccg                                                                                                                                                                       >  2:13611/1‑237 (MQ=255)                                                                                                                           cGCTTGTTGAGTCGGCGTTTTGTTCAGATAGTCCGCACGGAACAAGATGTTGCTGCTAATGTCGGGCACAGTATCGTAAAGATCGAGCTGGTTGTTCATTTCCGGTATGCCGCCATGAATCATCCAGTCTGTCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGTCGGTTTAACGACATCCGCCCACCATTTTGCCAAGACGTCATAACGCGCGTCACTcc                                                                                                             <  2:83300/251‑1 (MQ=255)                                                                                                                                                           ccTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCATTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGTCGGTTTAACGACATCCGCCCACCATTTTGCCAACACGTCATAACGCGCGGCACTCCGTGAGAACGGCCAGTAAATTTGGGGAGCAATg                                                                             <  1:238213/251‑1 (MQ=255)                                                                                                                                                                              gCCGCTAATTGCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCATTAATCATCCAGTTTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTTGTCGGTTTAACGACATCCGCCCACCATTTTGCCAACACGTCATAACGCGCGGCACTCCGTGAGAACGGCCAGTAAATTTGGGGAGCAATGTAATCCAGCAATCCTTGtt                                                          <  1:314560/251‑1 (MQ=255)                                                                                                                                                                                                          aGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCATTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGTCGGTTTAACGACATCCGCCCACCATTTTGCCAACACGTCATAACGCGCGGCACTCCGTGAGAACGGCCAGTAAATTTGGGGAGCAATGTAATCCAGCAATCCTTGTTCCACCCATCGACGGGTGTCAGCGTAGGa                              <  2:79990/251‑1 (MQ=255)                                                                                                                                                                                                                    gctTTTTCAGTTCCGGTACGCCGCCATGAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGTCGGTTTAACGACATCCGCCCACCATTTTGCCAACACGTCATAACGCGCGGCACTCCGTGAGAACGGCCAGTAAATTTGGGGAGCAATGTAATCCAGCAATCCTTGTTCCACCCATCGACGGGTGTCAGCGTAGGATTCGTCATa                     <  1:291089/250‑1 (MQ=255)                                                                                                                                                                                                                       ttttCAGTTCCGGTACGCCGCCATTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGTCGGTTTAACGACATCCGCCCACCATTTTGCCAACACGTCATAACGCGCGGCACTCCGTGAGAACGGCCAGTAAATTTGGGGAGCAATGTAATCCAGCAATCCTTGTTCCACCCATCGACGGGTGTCAGCGTAGGATTCGTCATAgg                   <  1:64936/249‑1 (MQ=255)                                                                                                                                                                                                                        tgtcattgccGGTACGCCGCCATTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCTGGTCCTGGTCGTTTTAACGACATCCGCCCACCATTTTGCCAACACGTCATAACGCGCGGCACTCCGTGAGAACGGCCAGTAAATTTGGGGAGCAATTTTATCGAGCAATCCGTGTTCCACCCATCGACTGGTTTCAGCTTAGGAGTCGTCATAGGCt                 <  2:166920/242‑1 (MQ=255)                                                                                                                                                                                                                                      cgccgcCATTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGTCGGTTTAACGACATCCGCCCACCATTTTGCCAACACGTCATAACGCGCGGCACTCCGTGAGAACGGCCAGTAAATTTGGGGAGCAATGTAATCCAGCAATCCTTGTTCCACCCATCGACGGGTGTCAGCGTAGGATTCGTCATAGGCTGCCGCGCCTCGGGTa  <  2:124776/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    TAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGTCGGTTTAACGACATCCGCCCACCATTTTGCCAACACGTCATAACGCGCGGCACTCCGTGAGAACGGCCAGTAAATTTGGGGAGCAATGTAATCCAGCAATCCTTGTTCCACCCATCGACGGGTGTCAGCGTAGGATTCGTCATAGGCTGCCGCGCCTCGGGTA  >  NC_000913/1567415‑1567894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑