Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,567,076	G→A	P22L (CCC→CTC)	dosC ←	diguanylate cyclase DosC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,567,076	0	G	A	100.0%	57.5 / NA	20	P22L (CCC→CTC)	dosC	diguanylate cyclase DosC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/10);  total (10/10)

CTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATT  >  NC_000913/1566837‑1567319                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    cTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGAGCGGATCTGcc                                                                                                                                                                                                                                          >  1:74340/1‑251 (MQ=255)                     gcgACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGAGCGGATCTGCCTGTTCGACAAGTCCGGTCCa                                                                                                                                                                                                                      <  2:117955/251‑1 (MQ=255)                               gCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGAGCGGATCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTc                                                                                                                                                                                                            >  2:261209/1‑251 (MQ=255)                                                          cGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCATCTGACTCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGAGCGGATCTGCCTGTTCGACAAGTCCTGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAAtt                                                                                                                                                                                 <  2:163893/251‑1 (MQ=255)                                                                                  taataaTCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGAGCGGATCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGCTGGGAAAt                                                                                                                                                         >  2:263727/1‑251 (MQ=255)                                                                                                      gCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGAGCGGATCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGaa                                                                                                                                      >  2:123049/1‑250 (MQ=255)                                                                                                                         cAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGAGCGGATCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCg                                                                                                                    <  1:123049/249‑1 (MQ=255)                                                                                                                                      aCTCAAGAATGCTTCGTCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATAATCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGAGCGGATCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGCTGGTAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCata                                                                                                     <  2:318385/251‑1 (MQ=255)                                                                                                                                         cAAGAATTCTTCGGCAGTCAGGTTGATGCGGACAATACGAGAAAACTCAATACTCAAATAAGGAGCAAGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGAGCGGATCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCTCACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTTCTCCatatc                                                                                                   <  2:306929/250‑2 (MQ=255)                                                                                                                                                             ggTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGAGCGGATCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGAtata                                                                              <  1:211165/251‑1 (MQ=255)                                                                                                                                                             ggTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCATGCGCAAAGGCAATTTCCGCGGCTTTAGCACGAATGAGAGGATCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCAATAATTCACACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGatat                                                                               >  1:46504/1‑250 (MQ=255)                                                                                                                                                                 gATGCGGACAATTCGATAAACCTCGATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGAGCGGATCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTg                                                                           >  1:83300/1‑250 (MQ=255)                                                                                                                                                                                     aCTCGATACTCAGATAATGGGCATGCGCAACGGCATTTTCCGCGGCTTTAACATGAATGAACGGAGCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACTCCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTCAAATATGAAGATATACTGGAGAGAAATATGCGATTTAgg                                                      >  1:62916/1‑251 (MQ=255)                                                                                                                                                                                         gATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGAGCGGATCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGat                                                                                 <  1:48095/220‑1 (MQ=255)                                                                                                                                                                                         gATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGAGCGGATCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGat                                                                                 >  2:48095/1‑220 (MQ=255)                                                                                                                                                                                                             gcgcAACGGCAATTTCCGCGGCTTTAGCACGAATGAGCTGATCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGtt                              <  2:74340/251‑1 (MQ=255)                                                                                                                                                                                                                   cGGCAATTTCCGCGGCTTTAGCACGAATGAGCGTATCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCGTAATTCACACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAATCGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATAGACTGAAAAGAAATAAGCTATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCag                        <  1:218690/251‑1 (MQ=255)                                                                                                                                                                                                                     gCAATTTCCGCGGCTTTAGCAAGAATTAGCGGATCTGCATGTTCGAAACGTCCGGTCCTCTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACATTTTCAATCTAAGCTACTGTTATTCagaa                      <  2:113810/251‑1 (MQ=255)                                                                                                                                                                                                                      cAATTTCCGCGGCTTTAGCACGAATGAGCGGATCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTCATTCTTTGAAAATACAACTCCATAATTCACACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGTACAGTTTCAATCTACGCTACTGTTCTTCaga                       >  1:374795/1‑249 (MQ=255)                                                                                                                                                                                                                                          cGAATGAGCGGATCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAatt  >  2:35479/1‑250 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    CTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGTCAACCTGGGCAGAAAGCACGTTAATAATCCAGCGTTCCATCGCACTCTTCAACTGCCGCTCAACTTGTTCATTACTCAAGAATTCTTCGGCATGCGGGTCGATGCGGACAATTCGATAAAACTCGATACTCAGATAATGAGCATGCGCAACGGCAATTTCCGCGGCTTTAGCACGAATGGGCGGATCTGCCTGTTCGACAAGTCCGGTCCACTCATCTTTCATTCTTTTAAAATACATCTCCATAATTCACACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATT  >  NC_000913/1566837‑1567319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑