Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,552,228	G→A	intergenic (+237/+170)	fdnI → / ← yddM	formate dehydrogenase N subunit gamma/DNA‑binding transcriptional regulator YddM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,552,228	0	G	A	100.0%	105.7 / NA	32	intergenic (+237/+170)	fdnI/yddM	formate dehydrogenase N subunit gamma/DNA‑binding transcriptional regulator YddM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/17);  total (15/17)

ATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGG  >  NC_000913/1551988‑1552465                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              ataATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGCAgg                                                                                                                                                                                                                                     >  1:349947/1‑251 (MQ=255)          tttGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGCAGGCTTTGtt                                                                                                                                                                                                                              <  2:349947/249‑1 (MQ=255)           ttGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCAt                                                                                                                                                                                                                           >  1:155868/1‑251 (MQ=255)            tGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCCGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAAAAACGTAGGCTTTGTTCATg                                                                                                                                                                                                                          >  1:165839/1‑251 (MQ=255)                  ttAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGa                                                                                                                                                                                                                     <  1:88133/250‑1 (MQ=255)                                          aGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAAAGCCTTATCCGGc                                                                                                                                                                                             >  1:285262/1‑250 (MQ=255)                                          aGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAAGGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCa                                                                                                                                                                                            <  1:260025/251‑1 (MQ=255)                                                cATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACCACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGaaa                                                                                                                                                                                       >  2:48603/1‑250 (MQ=255)                                                         atTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCaa                                                                                                                                                                              <  1:48603/250‑1 (MQ=255)                                                              aGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCa                                                                                                                                                                         >  1:282245/1‑250 (MQ=255)                                                                      tAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTAACCGGCCTGAAAAACCTTCCAATCCAATAAATTGCAGTGAACGTGTAGGCCTGATAAGCGTTGCGCATCGGGCAATGCTGCGGTAGACATCAGTTTCAAATGGCGCTGCAAAAGGCGGCATTTTATTACTGTAACCATCGGAGGCTTTGTCAATGCCGGACGCGGCGTCAAAGCGTTAACCGGCATGGAAACCCTTCAAATCCAATAGAGTGc                                                                                                                                                                >  1:229104/1‑251 (MQ=12)                                                                            ttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGa                                                                                                                                                           <  1:80251/250‑1 (MQ=255)                                                                              ggtCATGCCGGATGCCGCGAGAACGGCTTATCCGGCATGAAAACACTTCAAATACAATAGATTGCAGTGACCGGGTCGGCCTGGGAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCCGTTTCCAATGGCGCTGTAACAGGCGTCATTTTCCTATTGCAACCAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACTCCCTATCCCGCATGAACAACCTTCAAAtgcactcgatttcagagaa                                                                                                                                                          >  2:397584/3‑231 (MQ=16)                                                                                 cATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACgtg                                                                                                                                                      <  1:397584/250‑1 (MQ=255)                                                                                        gATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATTCGGCGTGAACGCCCTATCCGGCATGAAAAACCTTCAAATCCCAACGATTGCAGTGAACGTGTAGGCCTg                                                                                                                                              >  2:222760/1‑251 (MQ=25)                                                                                                   agcggcgtATCCGGAATGAAAACCCGTCAAAACCAATAGATTGCAGTGACAGTGTAGACCTGAGAAGAGTAGCGCATCAGGCAATGTTGTTTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAgc                                                                                                                                   <  2:1035/244‑1 (MQ=18)                                                                                                           aTCCGGCATGAAAACCCTTCAAATCCAAGAGATTGCAGTGAACGTGTAGGCCTGATAAGCGGAGCGCATCAGGTAATGTTGCGTTTTTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAg                                                                                                                            <  2:399834/250‑1 (MQ=255)                                                                                                                      aaaCACTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGt                                                                                                                <  1:204663/251‑1 (MQ=255)                                                                                                                            ttCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGtc                                                                                                           >  1:101635/1‑250 (MQ=255)                                                                                                                                      aTAGATTGCAGTGATCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGTATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGATCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTaa                                                                                                <  1:371418/251‑1 (MQ=255)                                                                                                                                                  gAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTtat                                                                                    <  1:20012/251‑1 (MQ=255)                                                                                                                                                       tgtAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTTAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGAGTAGGCCTGATAAGCGTAGCGCATCAGACAATGTTGCGTTTGTCCTCAGTTCTAAATGGCGCTTTATaacgg                                                                               >  2:40505/1‑248 (MQ=255)                                                                                                                                                           ggCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGAtt                                                                                                                                                                  <  2:228554/164‑1 (MQ=255)                                                                                                                                                           ggCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGAtt                                                                                                                                                                  >  1:228554/1‑164 (MQ=255)                                                                                                                                                             ccTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAGCCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCAtt                                                                         <  2:260370/251‑1 (MQ=255)                                                                                                                                                                                          tGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAATTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATc                                            >  1:206521/1‑251 (MQ=255)                                                                                                                                                                                           gCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATc                                            <  2:285262/250‑1 (MQ=255)                                                                                                                                                                                              tttGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATaa                                                                                                                                          >  1:382538/1‑153 (MQ=255)                                                                                                                                                                                              tttGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATaa                                                                                                                                          <  2:382538/153‑1 (MQ=255)                                                                                                                                                                                                tGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGac                                       <  2:169304/250‑1 (MQ=255)                                                                                                                                                                                                                              gCGTCATTTTCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGCCGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGAc        >  1:214169/1‑251 (MQ=255)                                                                                                                                                                                                                                     tttCATATTGTAAACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAgg  <  1:291925/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              ATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGG  >  NC_000913/1551988‑1552465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑