Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,551,766	C→T	F143F (TTC→TTT)	fdnI →	formate dehydrogenase N subunit gamma

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,551,766	0	C	T	100.0%	67.5 / NA	21	F143F (TTC→TTT)	fdnI	formate dehydrogenase N subunit gamma
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/10);  total (11/10)

GATGTTTATGTTTGTGCGTTTTGTGCATCACAACATCCCGGATAAGAAAGATATTCCGTGGCTGTTGAACATTGTCGAAGTATTGAAAGGCAATGAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTCCCGATGCAGGTTGTTCGCTACAGCCTGCTGATCCACGCGGCTGCGGGTATCATCCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTT  >  NC_000913/1551541‑1551999                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          gATGTTTATGTTTGTGCGTTTTGTGCATCACAACATCCCGGATAAGAAAGATATTCCGTGGCTGTTGAACATTGTCGAAGTATTGAAAGGCAATGAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTTCCGATGCAGGTTGTTCGCTACAGcc                                                                                                                                                                                                                  <  1:89080/251‑1 (MQ=255)                                    tCCCGGATAAGAAAGATATTCCGTGGCTGTTGAACATTGTCGAAGTATTGAAAGGCAATGAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTTCCGATGCAGGTTGTTCGCTACAGCCTGCTGATCCACGCGGCTGCGGGTATCATCCTGATc                                                                                                                                                                               >  1:12183/1‑251 (MQ=255)                                     cccGGATAAGAAAGATATTCCGTGGCTGTTGAACATTGTCGAAGTATTGAAAGGCAATGAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTTCCGATGCAGGTTGTTCGCTACAGCCTGCTGATCCACGCGGCTGCGGGTATCATCCTGATcc                                                                                                                                                                              <  2:217171/251‑1 (MQ=255)                                     cccGGATAAGAAAGATATTCCGGGGCTGTTGAACATTGTCGAAGTATTGAAAGGCAATGAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCGGGTTACCTGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTTCCGATGCAGGTTGTTCGCTACAGCCTGCTGATCCACGCGGCTGCGGGTATCATCCTGATcc                                                                                                                                                                              <  1:395992/251‑1 (MQ=255)                                        ggATAAGAAAGATATTCCGTGGCTGTTGAACATTGTCGAAGTATTGAAAGGCAATGAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTTCCGATGCAGGTTGTTCGCTACAGCCTGCTGATCCACGCGGCTGCGGGTATCATCCTGATCCACg                                                                                                                                                                           <  2:287602/251‑1 (MQ=255)                                               aaagaTATTCCGTGGCTGTTGAACATTGTCGAAGTATTGAAAGGCCATGAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATTTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTGGCGCTGTGCTTTCCGATGCAGGTTGTTCGCTACAGCCTGGTGATGCAGGCGGCTGCGGGTATCGTTCTGATTGTCGCCATCCt                                                                                                                                                                    <  2:157158/251‑1 (MQ=255)                                                 agaTATTCCGTGGCTGTTGAACAGTGTCGAAGTATTGAAAGGCAATGAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTTCCGATGCAGGTTGTTCGCTACAGCCTGCTGATCCACGCGGCTGCGGGTATCATCCTGATCCACGCCATCCTGa                                                                                                                                                                  <  2:187018/251‑1 (MQ=255)                                                                gTTGAACATTGTCGAAGTATTGAAAGGCAATGAGCATAAAGTGGCGGATGTCGTGAAGTACAACGCCGGGCAAAAGATGATGTTCTTGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTTGCGTCCGTACTTTGCGCAGTACTTTCCGATGCAGGTTGTTCGCTACAGCCTGCTGATCCACGCGGCTGCGGGTATCATCCTGATCCACGCCATCCGGATCCATATGTATATg                                                                                                                                                    <  1:275456/250‑1 (MQ=255)                                                                  tGAACATTGTCGAAGTATTGAAAGGCAATGAGCATAAAGTGGCGGATGTTGGTAAGTACAAAACCGGGCAAAAGATGATATTGTGGTAGATCATGAGAATGATTTTCGTGGTGCTGGGGACCGGGGGGGTTTTCTGGCGTTCGTACTTTGCGCAGTAATTTCCGAGGCAGGTTGTTCTCTACAGCCTGCTGATCCACGCGGCGGCGGGTATCATGCTGATTCCCGCCATCCTGTTCCaatttattgtggct                                                                                                                                                 >  2:20012/1‑238 (MQ=255)                                                                               aGTATTGAAAGGCAATGAGCATAAATTGGCGGATGTCGGTAAGTGCAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGGCCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTTCCGATGCAGGTTGTTCGCTACAGCCTGCTGATACACGCGGCTGCGGGTATCATCCTGATCCACGCCATCCTGATCCATATGTATATGGCATTGTGGGTGAAAg                                                                                                                                    <  2:372832/251‑1 (MQ=255)                                                                                 tATTGAAAGGCAATGAGCATAAAGTGGCGGATGTCGGTAAGTACAAAGCCGGGCAAAAGCTGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGGGACCGGGGGGATTATCTGGCGTCCGTACTTTGCGCAGTACTTTCCGATGCAGGTTGTTCGCTACAGCCTGCTGATCCACGCGGCTGCGGGGATCATCCTGATCCACCCCATCCTCATCCATATGTTTTTGGCATTTTGGGGGAAAggg                                                                                                                                  >  2:224516/1‑250 (MQ=255)                                                                                       aaGGCAATGAGCATAAAGTGGCGGATGTCGGTAAGGACAACGCCGGGCAAAAGATGATGTTCTGGGCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTTTCTGGCGTCCGTACTTTGCGCAGTACTTTCCGATGGAGGTTGTTCGCTACAGCCTGTTGATCCACCCGGCCGCGGGTATCATCCTGATCCCCGCCCTCCTGATCCATATGTATATGTCGTTTTTGGGGAAAAGATCTAtt                                                                                                                            >  2:297616/1‑251 (MQ=255)                                                                                                             ggATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTTCCGATGCAGGTTGTTCGCTACAGCCTGCTGATCCACGCGGCTGCGGGTATCATCCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCgaagggaagg                                                                                                      >  2:260025/1‑251 (MQ=255)                                                                                                                                               atgTTCTGGTCGAACATGAGCATGATTTTCGTGCTGCTGGAGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTTCCGATGAAGGTTGTTCGCTACAGCCTGCTGATCAACGCGGCTGCGGGTATCATCCTGATCCACGCCATCCTGATCCATATGAATATGGCATTTTGGGCGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACTCCATCcgcg                                                                    >  2:371418/1‑251 (MQ=255)                                                                                                                                                  ttCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTTCCGATGCAGGTTGTTCGCTACAGCCTGCTGATCCACGCGGCTGCGGGTATCATCCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGTTTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTg                                                                 >  2:173998/1‑251 (MQ=255)                                                                                                                                                           aTCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTTCCGATGCAGGTTGTTCGCTACAGCCTGCTGATCCACGCGGCTGCGGGTATCATCCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTg                                                         >  2:101018/1‑250 (MQ=255)                                                                                                                                                                gAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTTCCGATGCAGGTTGTTCGCTACAGCCTGCTGATCCACGCGGCTGCGGGTATCATCCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCg                                                   <  1:101018/251‑1 (MQ=255)                                                                                                                                                                             gTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTTCCGATGCAGGTTGTTCGCTACAGCCTGCTGATCCACGCGGCTGCGGGTATCATCCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGc                                      >  2:88133/1‑251 (MQ=255)                                                                                                                                                                                  gctgGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTTCCGATGCAGGTTGTTCGCTACAGCCTGCTGATCCACGCGGCTGCGGGTATCATCCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGaa                                   <  2:12183/249‑1 (MQ=255)                                                                                                                                                                                                  aTTATCTGGCGTCCGTACTTTGCGCAGTACTTTCCGATGCAGGTTGTTCGCTACAGCCTGCTGATCCACGCGGCTGCGGGTATCATCCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAgg                 >  1:230784/1‑251 (MQ=255)                                                                                                                                                                                                                 tACTTTGCGCAGTACTTTCCGATGCAGGTTGTTCGCTACAGCCTGCTGATCCACGCGGCTGCGGGTATCATCCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCtttt  >  2:80251/1‑251 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          GATGTTTATGTTTGTGCGTTTTGTGCATCACAACATCCCGGATAAGAAAGATATTCCGTGGCTGTTGAACATTGTCGAAGTATTGAAAGGCAATGAGCATAAAGTGGCGGATGTCGGTAAGTACAACGCCGGGCAAAAGATGATGTTCTGGTCGATCATGAGCATGATTTTCGTGCTGCTGGTGACCGGGGTGATTATCTGGCGTCCGTACTTTGCGCAGTACTTCCCGATGCAGGTTGTTCGCTACAGCCTGCTGATCCACGCGGCTGCGGGTATCATCCTGATCCACGCCATCCTGATCCATATGTATATGGCATTTTGGGTGAAAGGATCGATTAAAGGGATGATCGAAGGGAAGGTAAGTCGTCGCTGGGCGAAGAAACACCATCCGCGCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTT  >  NC_000913/1551541‑1551999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑