Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,547,154	C→T	A6T (GCA→ACA)	yddG ←	amino acid exporter YddG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,547,154	0	C	T	100.0%	70.7 / NA	22	A6T (GCA→ACA)	yddG	amino acid exporter YddG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/12);  total (10/12)

ACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTT  >  NC_000913/1546917‑1547358                                                                                                                                                                                                                                              |                                                                                                                                                                                                             aCCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCgtgt                                                                                                                                                                                                  <  1:219234/250‑1 (MQ=255)                  agacagaTTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCCAGGAGCGATGTTTTTTGTCGTGTCATGCTCGCTGTTTTGtc                                                                                                                                                                                >  2:245364/1‑251 (MQ=255)                                   gACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTaaa                                                                                                                                                                 <  1:146054/249‑1 (MQ=255)                                    aCGAATACCAGACTCCCGGCGCGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAGCCGTGAAGCTTACCAGCAGCCCGCTTAATGAATAGATAGCAACTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCTTCGTGCTCCCCAGGACGATCGCTATCAGCCCTATGAACGTTGTTTTTTGTCGTGTCATGCTCGTTGTTTTGTCTCTCTTGCCGTTaaaatt                                                                                                                                                              <  2:152421/251‑3 (MQ=255)                                           aCAGCCTCCCTGCGGGTAAATTGCCTTTCGGGATGTTCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATTAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCTAATCAATCCTACCATCGTGCTCCACAGTACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTATCGTGTCATGCTCGCTGTTTTGTCTCTCTTTCCGTTAAAAATTAAGCTg                                                                                                                                                       <  2:314747/251‑1 (MQ=255)                                              gACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAAt                                                                                                                                                    <  1:201788/251‑1 (MQ=255)                                                       cGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCa                                                                                                                                           <  1:110926/251‑1 (MQ=255)                                                                                    aTACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAgtggt                                                                                                              >  1:388843/1‑251 (MQ=255)                                                                                            aaaTCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCTCGCTGTGTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGttttt                                                                                                      >  1:287062/1‑251 (MQ=255)                                                                                                    ccGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCt                                                                                              <  1:68920/251‑1 (MQ=255)                                                                                                                 cagcagCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTc                                                                                 <  1:148238/251‑1 (MQ=255)                                                                                                                  agcagcCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTc                                                                                 >  1:305115/1‑250 (MQ=255)                                                                                                                             gaaTGAATAGATAGCAGCTGCGCCTCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTCAATTTGCGAAgg                                                                     >  2:43520/2‑251 (MQ=255)                                                                                                                                 gAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCGACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCgg                                                                  <  2:12083/250‑1 (MQ=255)                                                                                                                                     agatagCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGattat                                                             <  1:344611/251‑1 (MQ=255)                                                                                                                                       atagCAGCTGCGCCGACGACCGGGCCGAGCCCCTCCCTGACCCCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCACGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTAttt                                                           >  1:337466/1‑251 (MQ=255)                                                                                                                                                         aCCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTc                                         <  2:129486/251‑1 (MQ=255)                                                                                                                                                           cGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCtt                                       >  2:295973/1‑251 (MQ=255)                                                                                                                                                            gggCCGAGCCCCTCACTGACAGCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTAAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGGAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTAttt                                      >  1:297/1‑251 (MQ=255)                                                                                                                                                                                   gcgAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCa               <  2:127254/251‑1 (MQ=255)                                                                                                                                                                                      aatcaaccATACCATAGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAAATAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAAGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGa             >  2:231755/1‑250 (MQ=255)                                                                                                                                                                                                  cATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGTTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGtt  >  2:297148/1‑249 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                             ACCCTAAGGAAAGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTT  >  NC_000913/1546917‑1547358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑