Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,440,381	G→A	A135V (GCG→GTG)	ydbK ←	putative pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,440,381	0	G	A	89.2%	98.2 / ‑5.8	39	A135V (GCG→GTG)	ydbK	putative pyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  major base A (19/14);  minor base C (0/4);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 4.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGACCTGCGGCATGAGATCAAGAATCGTGTCATCGGCCAGCGGGACAATTTTATTGATTTCGTGGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑GCGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCA  >  NC_000913/1440137‑1440620                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                ggCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGACCTGCGGCATGAGATCAAGAATCGTGTCATCGGCCAGCGGGACAATTTTATTGATTTCGTGGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGCTTTTCAGCATCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAAc                                                                                                                                                                                                                                           >  2:36278/1‑251 (MQ=255)    ggACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGACCTGCGGCATGAGATCAAGAATCGTGTCATCGGCCAGCGGGACAATTTTATTGATTTCGTGGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCg                                                                                                                                                                                                                                        >  2:189043/1‑251 (MQ=255)          aCCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGACCTGCGGCATGAGATCAAGAATCGTGTCATCGGCCAGCGGGACAATTTTATTGATTTCGTGGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTgg                                                                                                                                                                                                                                   <  2:350890/250‑1 (MQ=255)                gATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGACCTGCGGCATGAGATCAAGAATCGTGTCATCGGCCAGCGGGACAATTTTATTGATTTCGTGGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTGGCGCACCg                                                                                                                                                                                                                            <  1:189043/251‑1 (MQ=255)                gATCACCGGATGTTCCGGGTGGAGTGCCCGGGCGCGATGAGCATCAATTTCTACCTGCGGCATGAGATCAAGAATCGTGTCATCGGCCAGCTGGACAAGTTTATTGATTTCGTGGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTAT‑CTGCACACATCA‑TC‑ACGCAACCCGTCTGGGGCACCg                                                                                                                                                                                                                            <  2:256524/251‑1 (MQ=255)                   cACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGACCTGCGGCATGAGATCAAGAATCGTGTCATCGGCCAGCGGGACAATTTTATTGATTTCGTGGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑CCGCAACCCGGCTGGCGCACCGcc                                                                                                                                                                                                                          <  2:253809/250‑1 (MQ=255)                   cACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGACCTGCGGCATGAGATCAAGAAGCGTGTCATCGGCCAGCGGGACAATTTTATTGATTTCGTGGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTGGCGCACCGcc                                                                                                                                                                                                                          <  2:338661/250‑1 (MQ=255)                               cgggtttggTGCCCGGGCGCGAGGAGTATCAATTTCGACCGGCGGCATGAGATCAAGAATCGTGTCATCGGCCAGCGGGACAATTTTATGGATTTCGTGGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGCCGTTTG‑CTGCACAAAGCA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAAt                                                                                                                                                                                                             <  1:172960/243‑1 (MQ=255)                                                            caagttcgccctgcggcagtataccAAGAATCGTGTCATCGTCCATCGGGACAATTTTATTGATTTCGTGGGACGTGCGGAACCCAACAAAGAAATGAATAAAGGGCACGCGGCTTTTCAGCGCCGCGATTTGCGAAAT‑CAGAGAAAAGTCTTGCGCTTCCTGGCCTTTTG‑CTGCCCACAACA‑TC‑CCCCAACCCGTGTGACCCACCGCCATAACGTCGGAATGATCGCCAAAAAGAGATAGTGCAtgtgt                                                                                                                                                                                 <  2:30515/226‑1 (MQ=255)                                                                         cGGCATGAGATCAAGAATCGTGTCATCGGCCAGCGGGACACTTTTATTGATTTCGTGGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTGGCGCAACGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGCGTGGCAACGGTACGtt                                                                                                                                                                   >  1:222357/1‑250 (MQ=255)                                                                            cATGAGATCAAGAATCGTGTCATCGGCCAGCGGGACAATTTTATTGATTTCGTGGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGTTTTTCAGCGTCGCGTTTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTCCCGGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCg                                                                                                                                                                <  1:196487/251‑1 (MQ=255)                                                                                 attaaagcaTCGCGTCATCGGTCAGCGTGATAATTTTATTGATTGCGTGGTACGTGCGGAAATCATCAAAGAAATGAATAAGTGGAACGGGGCTTTCCAGCTTCGCCATTTGCGAAAT‑AATACCAAAGTCTTTGGCTCCCTTGACTTTTG‑CTGCCCACAACC‑TC‑ACGCAACCTGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAATTAGAGAGTGCATGTGTGTCAACGTTACGTGCCGCTACa                                                                                                                                                           <  1:66561/243‑1 (MQ=255)                                                                                  ataaaGAATCGTTACATCGGCCAGCGGAACAATTTTATTGGTTTCGTGGGACGTGCGGAAACCAACAAAGAAAGAAATAAATGGCACGCGGCTTTTCAGCGTCGCGATTTGAGAAAT‑GAGAGCAAAGTCTTGCCCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACCCACCCGGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACAt                                                                                                                                                          <  2:347670/248‑1 (MQ=255)                                                                                  aTCAAGAATCGTGTCATCGGCCAGCGGGACAATTTTATTGATTTCGTGGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACAt                                                                                                                                                          >  1:137810/1‑251 (MQ=255)                                                                                  aTCAAGAATCGTGTCATCGGCCAGCGGGACAATTTTATTGATTTCGTGGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGAACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAAAA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACAt                                                                                                                                                          >  1:231340/1‑251 (MQ=255)                                                                                      aGAATCGTGTCATCGGCCAGCGGGAAAATTTTATTGATTTCGTGGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGTTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCGGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCGGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAg                                                                                                                                                      <  1:378481/251‑1 (MQ=255)                                                                                         aTCGTGTCATCGGCCAGCGGGACAATTTTATTGATTTCGTGGGACGTGCGGAAACCATCAAAGAAGTGAATAAATGGCACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTTCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGAc                                                                                                                                                   >  2:99397/1‑251 (MQ=255)                                                                                           cGTGTCATCGGCCAGCGGGACAATTTTATTGATTTCGTCGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGCGCATGTGTGGCAACGGTACGTGCCGCTCCATGCAGGAcc                                                                                                                                                  >  2:133018/1‑249 (MQ=255)                                                                                                   gggacggggggaaatttttATGGATTTCGTGGGGCGTGCGGAAACCATAAAAGAAATGAATAAATGGCTCGCGGCTTTTCCGCGTGGCGATTGGGGAAAG‑GAGAGCAAAGTTTCGCTCTCCCGGGGCGTTGG‑GTGCACAAAACA‑CC‑ACGCAACCGGTCGGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGtt                                                                                                                                         <  2:148394/236‑1 (MQ=255)                                                                                                   cGGCCAGCGGGACAATTTTATTGATTTCGTGGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑GCGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTAAATGCAGGCAAAACGGTGtt                                                                                                                                         >  2:219808/1‑251 (MQ=255)                                                                                                       cAGCGGGACAATTTTATTGATTTCGTGGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAACTAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGtt                                                                                                                                     >  1:165515/1‑251 (MQ=255)                                                                                                                         gttttCGGGGGACGGGCGAAAACCACAAAAGAAATGAAAAAATGGACCGGGTGTTTCCAGCGCCGCGATTTGCAAAAT‑GAGAGAAAAGTCTGCCGCTTCCTGGACGTTTG‑CGGC‑CACAAAAACC‑CCGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGGTCGCCTGCCAGTTTGTACa                                                                                                                   <  1:103077/249‑1 (MQ=255)                                                                                                                                        gCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGTACAGCGTCGGGatcatc                                                                                                     >  2:13578/1‑250 (MQ=255)                                                                                                                                                 acaaaaaaaaTGAATAAATGGCCCGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTGG‑CTGCACACAACA‑TC‑ACGCACCCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGTACAGCGTCGGGATCATCAGCAGCAAAc                                                                                           <  1:115837/244‑1 (MQ=255)                                                                                                                                                    aaaGAAATGAAAAAAGGGCACGTGGTTTTTCGCCGTGGCG‑TTTGCCAAATTGAGAGAAAAGTCTGGCGCT‑CCCGGGTGTTTGGCTGCACACACCA‑TCAGC‑CACCCCGTGGGGCGCGCCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGGGCATGTGGGGCAACGGTACGTGCCGCTACATGAAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCt                                                                                        <  2:66147/251‑1 (MQ=255)                                                                                                                                                         aataaaTAAATGGCACGCGGCTTTTCAGCTTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCCCACAACA‑CC‑CCGCACCCCGTCTGGCGCCCCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGAt                                                                                   <  1:378475/247‑1 (MQ=255)                                                                                                                                                          agaaataaagggcagccgtcTTTTCAGCGTCGCAATTTGCAAAAC‑GAGAGCAAAGTCTTGCGTTGCCGGGAGGTGTG‑CTGAACCCAACA‑CC‑ACGCACCCCGTCTGGCACGCCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGGACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATg                                                                                  <  1:104348/232‑1 (MQ=255)                                                                                                                                                                  aTGGCACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAAc                                                                          >  1:65797/1‑251 (MQ=255)                                                                                                                                                                               tttCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATg                                                                                  >  1:199359/1‑230 (MQ=255)                                                                                                                                                                                      gTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGt                                                      >  2:11164/1‑251 (MQ=255)                                                                                                                                                                                      gTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGt                                                      >  1:271646/1‑251 (MQ=255)                                                                                                                                                                                        cgcgATTTGCGAAAT‑GAGAGCAGAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACACCA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAACATAGAGAGTGCATGTGTGGCAACGGCACGTTCCGCTACATGCAGGACAAACGGTGTTAGTTCGACTGCCAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGACAGGGCAACCGTCt                                                    >  2:314314/1‑251 (MQ=255)                                                                                                                                                                                                    aaT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCAGTCTGCAAAGCGCCAt                                        >  2:196463/1‑251 (MQ=255)                                                                                                                                                                                                    aaT‑GAGAGCAAAGTCTTGCGCTCCCTGGACTTTTG‑CTGCACACAACA‑TC‑ACGCACCCCTTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCAt                                        <  1:284573/251‑1 (MQ=255)                                                                                                                                                                                                                    ttGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGAt                           >  2:59061/1‑249 (MQ=255)                                                                                                                                                                                                                              gggACTTTTG‑CGGCACACAACA‑AC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTTCATGCAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTc               <  1:234838/250‑1 (MQ=255)                                                                                                                                                                                                                                gACGTTTG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCg             >  1:295262/1‑251 (MQ=255)                                                                                                                                                                                                                                    tttG‑CTGCACACAACA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTg         >  2:13092/1‑251 (MQ=255)                                                                                                                                                                                                                                             acacaACA‑TC‑ACGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCa  >  2:83390/1‑250 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                GGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGACCTGCGGCATGAGATCAAGAATCGTGTCATCGGCCAGCGGGACAATTTTATTGATTTCGTGGGACGTGCGGAAACCATCAAAGAAATGAATAAATGGCACGCGGCTTTTCAGCGTCGCGATTTGCGAAAT‑GAGAGCAAAGTCTTGCGCTTCCTGGACGTTTG‑CTGCACACAACA‑TC‑GCGCAACCCGTCTGGCGCACCGCCATAACGTCGGAATGATCGCCAAAAATAGAGAGTGCATGTGTGGCAACGGTACGTGCCGCTACATGCAGGACAAACGGTGTTAGTTCGCCTGCCAGTTTGTACAGCGTCGGGATCATCAGCAGCAAACCCTGCGATGACGTAAACGATGTTGAAAGGGCACCCGTCTGCAAAGCGCCATGCACGGTAGCGATAGCACCCGCTTCCGACTGCATTTCA  >  NC_000913/1440137‑1440620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑