Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,845	C→T	S300L (TCG→TTG)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,845	0	C	T	100.0%	105.0 / NA	29	S300L (TCG→TTG)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/16);  total (13/16)

CCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGCGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCATCGCATCTGGCATTCAGGCAAGGTAGCTGGTATTTATTTCAGCGTCATATGCGTGGCAACGGTAATATTCTGTGGTGACGGTTTTCCAGAAATTAAGCGGAATAATAACTCGCAGCTTTGTTGACCTAACTCCTGCGTCGGAACATC  >  NC_000913/1382601‑1383076                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        ccGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGCGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGGGTCGGCGTAAATTACTTGTTCtt                                                                                                                                                                                                                                   >  1:287666/1‑250 (MQ=255)         gCTCGGCAACCACTCGGTGACGCCGTATACGCGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATggg                                                                                                                                                                                                                           <  1:211653/251‑1 (MQ=255)              gCAACCACTCGGTGACGCCGTATACGCGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTaa                                                                                                                                                                                                                       >  1:225422/1‑250 (MQ=255)               cAACCACTCGGTGACGCCGTATACGCGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAAt                                                                                                                                                                                                                     >  2:176400/1‑251 (MQ=255)                            aCGCCGTATACGCGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATgcggc                                                                                                                                                                                                        <  1:290813/251‑1 (MQ=255)                                cGTATACGCGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTGTATTTTACGGTTATGATTGCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATGCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGa                                                                                                                                                                                                    <  2:95525/251‑1 (MQ=255)                                 gTATACGCGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGAt                                                                                                                                                                                                                                 >  1:181723/1‑221 (MQ=255)                                 gTATACGCGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGAt                                                                                                                                                                                                                                 <  2:181723/221‑1 (MQ=255)                                              gggCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGa                                                                                                                                                                                       <  2:82420/250‑1 (MQ=255)                                                                          ggCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGGTGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATgcg                                                                                                                                                           <  1:11308/250‑1 (MQ=255)                                                                           gCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCt                                                                                                                                                         <  1:88078/251‑1 (MQ=255)                                                                                      ttGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCTCTAgcatcgcctc                                                                                                                                              >  2:190482/1‑248 (MQ=255)                                                                                              gAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCATCGCATCTGGCATTc                                                                                                                                      >  1:357849/1‑251 (MQ=255)                                                                                                                       gTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCATCGCATCTGGCATTCAGGCAAGGTAGCTGGTATTTATTTc                                                                                                             <  2:215694/251‑1 (MQ=255)                                                                                                                            ttttACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCATCGCATCTTGCATTCAGGCAAGGTAGCTGGTATTTATTTCAGCGt                                                                                                        >  1:268503/1‑251 (MQ=255)                                                                                                                                     tATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCATCGCATCTGGCATTCAGGCAAGGTAGCTGGTATTTATTTCAGCGTCATATGCGt                                                                                               >  1:3022/1‑251 (MQ=255)                                                                                                                                        gATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCATCGCATCTGGCATTCAGGCAAGGTAGCTGGTATTTATTTCAGCGTCATATGCGTGGc                                                                                            >  1:313175/1‑251 (MQ=255)                                                                                                                                                        tttCCGTTTCGATCGAATACGCGTTTGAGTGGCAGGATCACGACGCCGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTCCTTGATCTGATACTGGGCTAAATTGCCGGATGCGGCGCGCGTACTTTATCCGATCTATAAATGTAGGCAGGATAAGATGCGCTCGCCTCGCATCTGGCATTCAGGCAAGGTAGCTGGTATTTATTTCACCGTCATATGCGTGGCAACGGTAATATTCtgt                                                                            >  2:248617/1‑251 (MQ=255)                                                                                                                                                           ccGTTTCGCTGGAATACGCGTTTGAGTGGCAGGAACACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCATCGCATCTGGCATTCAGGCAAGGTAGCTGGTATTTATTTCAGCGTCATATGCGTGGCAACGGTAATATTCTgtggt                                                                         >  1:340778/1‑251 (MQ=255)                                                                                                                                                              tttCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCATCGCATCTGGCATTCAGGCAAGGTAGCTGGTATTTATTTCAGCGTCATATGCGTGGCAACGGTAATATTCTGTGGTGAc                                                                      >  2:79744/1‑251 (MQ=255)                                                                                                                                                                                       gCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCATCGCATCTGGCATTCAGGCAAGGTAGCTGGTATTTATTTCAGCGTCATATGCGTGGCAACGGTAATATTCTGTGGTGACGGTTTTCCAGAAATTAAGCGGaata                                             <  1:71626/251‑1 (MQ=255)                                                                                                                                                                                                    gaAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCATCGCATCTGGCATTCAGGCAAGGTAGCTGGTATTTATTTCAGCGTCATATGCGTGGCAACGGTAATATTCTGTGGTGACGGTTTTCCAGAAATTAAGCGGAATAATAACTCGCAGCt                                <  1:282921/251‑1 (MQ=255)                                                                                                                                                                                                    gaAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCATCGCATCTGGCATTCAGGCAAGGTAGCTGGTATTTATTTCAGCGTCATATGCGTGGCAACGGTAATATTCTGTGGTGACGGTTTTCCAAAAATTAAGCGGAATAATAACTCGCAGCt                                <  1:79744/251‑1 (MQ=255)                                                                                                                                                                                                     aaGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCATCGCATCTGGCATTCAGGCAAGGTAGCTGGTATTTATTTCAGCGTCATATGCGTGGCAACGGTAATATTCTGTGGTGACGGTTTTCCAGAAATTAAGCGGAATAATAACTCGCAGCtt                               <  1:222918/251‑1 (MQ=255)                                                                                                                                                                                                            cAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCATCGCATCTGGCATTCAGGCAAGGTAGCTGGTATTTATTTCAGCGTCATATGCGTGGCAACGGTAATATTCTGTGGTGACGGTTTTCCAGAAATTAAGCGGAATAATAACTCGCAGCTTTGTTGAc                        <  1:380772/251‑1 (MQ=255)                                                                                                                                                                                                              gTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCATCGCATCTGGCATTCAGGCAAGGTAGCTGGTATTTATTTCAGCGTCATATGCGTGGCAACGGTAATATTCTGTGGTGACGGTTTTCCAGAAATTAAGCGGAATAATAACTCGCAGCTTTGTTGACCt                      <  2:340778/251‑1 (MQ=255)                                                                                                                                                                                                                       tCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAACTGTAGGCCGGATAAGATGCGCTATCATCGCATCTGGCATTCAGGCAAGGTAGCTGGTATTTATTTCAGCGTCATATGCGTGGCAACGGTAATATTCTGTGGTGACGGTTTTCCAGAAATTAAGCGGAATAATAACTCGCAGCTTTGTTGACCTAACTCCTGc             >  2:342290/1‑251 (MQ=255)                                                                                                                                                                                                                       tCCATTATGCAGGTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCATCGCATCTGGCATTCAGGCAAGGTAGCTGGTATTTATTTCAGCGTCATATGCGTGGCAACGGTAATATTCTGTGGTGACGGTTTTCCAGAAATTAAGCGGAATAATAACTCGCAGCTTTGTTGACCTAACTCCTg              <  2:253202/250‑1 (MQ=255)                                                                                                                                                                                                                                  ggTGTCGGCGTAAATTACTTGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCATCGCATCTGGCATTCAGGCAAGGTAGCTGGTATTTATTTCAGCGTCATATGCGTGGCAACGGTAATATTCTGTGGTGACGGTTTTCCAGAAATTAAGCGGAATAATAACTCGCAGCTTTGTTGACCTAACTCCTGCGTCGGAACATc  <  2:3022/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        CCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGCGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAGGATCACGACGAAGGCGACAGTGATAAATTCCATTATGCAGGTGTCGGCGTAAATTACTCGTTCTGATAATGGGCTAAATTGCCGGATGCGGCGCGAGTACTTTATCCGATCTATAAATGTAGGCCGGATAAGATGCGCTAGCATCGCATCTGGCATTCAGGCAAGGTAGCTGGTATTTATTTCAGCGTCATATGCGTGGCAACGGTAATATTCTGTGGTGACGGTTTTCCAGAAATTAAGCGGAATAATAACTCGCAGCTTTGTTGACCTAACTCCTGCGTCGGAACATC  >  NC_000913/1382601‑1383076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑