Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,366,985	C→T	G310R (GGG→AGG)	pspF ←	DNA‑binding transcriptional dual regulator PspF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,366,985	0	C	T	100.0%	85.6 / NA	24	G310R (GGG→AGG)	pspF	DNA‑binding transcriptional dual regulator PspF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/15);  total (9/15)

TATCCGCTGACCATCCCGGATGCGCAATTCGATGCGGCAATGAAAATTTTGCAGGATGCGCTGAGCGATTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACG  >  NC_000913/1366744‑1367220                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            tATCCGCTGACCATCCCGGATGCGCAATTCGATGCGGCAATGAAAATTTTGCAGGATGCGCTGAGCGATTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTc                                                                                                                                                                                                                                    >  1:342698/1‑251 (MQ=255)                  ggATGCGCAATTCGATGCGGCAAGGAAAATTTTGCAGGATGCGCTGAGCGATTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCTCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGt                                                                                                                                                                                                                   <  1:164387/251‑1 (MQ=255)                                            aaaTTTTGCAGGATGCGCTGAGCTATTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGt                                                                                                                                                                                         <  2:312386/251‑1 (MQ=255)                                                  tGCAGGATGCGCGGAGCGATTAATAGCCCAACGCAATAATGTCTGATTGGCTGCGATTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTgcag                                                                                                                                                                                    <  1:12348/250‑1 (MQ=255)                                                   gCAGGATGCGCTGAGCGATTAATAGCCCAACGCAATAAGGTCTGATGCGCGGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTgcagca                                                                                                                                                                                  <  2:201584/251‑1 (MQ=255)                                                      ggATGCGCTGAGCGATTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAAct                                                                                                                                                                               <  1:132722/251‑1 (MQ=255)                                                           cgcTGAGCGATTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCtttt                                                                                                                                                                          >  2:398992/1‑251 (MQ=255)                                                                    aTTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCtgctgc                                                                                                                                                                 <  1:333222/251‑1 (MQ=255)                                                                                                   gctgcgctTATAAGGCCTGAAAACGACGTATTGATTATGTATTCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTAGTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAgtg                                                                                                                                  <  1:265462/251‑1 (MQ=255)                                                                                                             tCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCg                                                                                                                         >  2:279056/1‑250 (MQ=255)                                                                                                                                        tgtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTc                                                                                             >  2:94264/1‑251 (MQ=255)                                                                                                                                        tgtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTc                                                                                             >  2:344413/1‑251 (MQ=255)                                                                                                                                                        cATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGt                                                                             <  1:70737/251‑1 (MQ=255)                                                                                                                                                                 cgcATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGa                                                                    <  2:53235/251‑1 (MQ=255)                                                                                                                                                                      ccGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGTAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACGGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGGTTAAAGGGATCaa                                                                <  2:214065/250‑1 (MQ=255)                                                                                                                                                                         gCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAGAGGGATCaataat                                                            >  1:296691/1‑251 (MQ=255)                                                                                                                                                                          cAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCGTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAatg                                                           <  1:398992/251‑1 (MQ=255)                                                                                                                                                                                gTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGtca                                                     <  1:304703/251‑1 (MQ=255)                                                                                                                                                                                    tGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAATCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGtcatca                                                  >  1:242124/1‑250 (MQ=255)                                                                                                                                                                                              cGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATc                                       <  2:280026/251‑1 (MQ=255)                                                                                                                                                                                                   aaTCTGGTGCTTTTTCAACAACCCGCGGAACTTATGATAAGTTAACCTCCGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGCGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGCATAATCGCTgg                                  >  2:369983/1‑251 (MQ=255)                                                                                                                                                                                                        ggTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGc                               <  2:304680/249‑1 (MQ=255)                                                                                                                                                                                                              ttttCAACAACGCGCGGAACTGATGATAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTTCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGTATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCg                        <  1:182277/250‑1 (MQ=255)                                                                                                                                                                                                                                   gatgatAAGTTAACCTCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACg  >  1:336250/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            TATCCGCTGACCATCCCGGATGCGCAATTCGATGCGGCAATGAAAATTTTGCAGGATGCGCTGAGCGATTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAACTCACGTAAATCCAGCGGCAGTGTTGGAAGCGAGGTGGTTTCTGAAACGGCGATAGCGTCTTCAGGCGGACGCCGTTTAAAGGGATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACG  >  NC_000913/1366744‑1367220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑