Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,362,803	C→T	R21C (CGC→TGC)	puuC →	gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,362,803	0	C	T	100.0%	92.8 / NA	29	R21C (CGC→TGC)	puuC	gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/15);  total (14/15)

CCGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATACGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCACTCTCTTCTCCGGCTAAACGTAAAGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGA  >  NC_000913/1362562‑1363038                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            ccGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATACGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCttattta                                                                                                                                                                                                                                    <  1:205944/251‑1 (MQ=255)  cGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATACGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCttatttat                                                                                                                                                                                                                                   <  2:297351/251‑1 (MQ=255)  cGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATACGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCttatttat                                                                                                                                                                                                                                   <  2:273997/251‑1 (MQ=255)            aCTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATACGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGa                                                                                                                                                                                                                          <  2:395575/250‑1 (MQ=255)            aCTCAGGTAAGTGAGTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATACGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGaa                                                                                                                                                                                                                         <  2:147246/251‑1 (MQ=255)                     aGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATACGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGGTAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATActgct                                                                                                                                                                                                                >  1:13481/1‑251 (MQ=255)                             gggggAGCGAACGCAGCCAAGTCAGAGGCGGCTTGAAGGATGAATTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATACGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCGCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGa                                                                                                                                                                                                        <  1:247715/251‑1 (MQ=255)                                ggAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATACGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCGGCGGCGGaaaa                                                                                                                                                                                                     <  2:84203/251‑1 (MQ=255)                                 gAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGCAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATACGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAAt                                                                                                                                                                                                    >  2:388187/1‑251 (MQ=255)                                         ataGCCAAGGCAGAGGCGGCTTGAAGGAGGAAGTGTATATAAAGATGGTCGCCAGAAATAAACGGGCATACGTCCCGGCTATCTCTGCGCACTGACGTTCACAAACTGCATATATCTGATAGACGTTAAACAGGAGTCATAATGAATGTTCATCATCTGTCTTACTGGCAGGATAAAGCGTTTAGTCTCGTCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCTGAAAATGAAACCtc                                                                                                                                                                                            <  2:162225/249‑2 (MQ=255)                                          cAGCCAAGGCAGAGGCGGCTTGAAGGATGAAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATACGGCCCGGGGATCTCTTCGCCCTGACGTTCACAAACTGCAGATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCttt                                                                                                                                                                                           <  1:263581/251‑1 (MQ=255)                                              cAAGGCAGAGGCGGCTTGAAGGATGAAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATACGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCttttaa                                                                                                                                                                                        >  2:297571/1‑247 (MQ=255)                                                 ggCAGAGGCGGCTTGAAGGATGAAGTGTATATAAAGAAGGTCGCCAGAAATAAACGGGCATATGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATAGATCTGATATACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAATCCg                                                                                                                                                                                    <  2:290721/251‑1 (MQ=255)                                                   cAGAGGCGGCTTGAAGGATGAAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATACGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGtt                                                                                                                                                                                  >  2:204093/1‑251 (MQ=255)                                                   cAGAGGCGGCTTGAAGGATGAAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATACGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGtt                                                                                                                                                                                  >  1:213536/1‑251 (MQ=255)                                                                   gATGAAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATACGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGTTGATCCGGTCACCCAgg                                                                                                                                                                  <  1:171613/251‑1 (MQ=255)                                                                          tggatataaagtatagagCCAGAACTAAACGGGCATACGGCCCGGTGATCTCTGCGCCCTGACGATCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCACGCTAACGCGTTAAGTCTCGCCATTGACAACTGCTTATTTATTAACGGTGAATATACTGCTGAGGCGGAAATTGAAACCTTTGAACCCGTTGGTCCGGTCACCCAGGCCCCGCt                                                                                                                                                           >  2:265773/18‑251 (MQ=255)                                                                            tatataAAGAATGTCGCCAGAAATAAACGGGCATACGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGTTGATCCGGTCACCTCGGCACCGCTgg                                                                                                                                                         >  2:197336/1‑251 (MQ=255)                                                                                                  aTAAACGGGCATACGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAagag                                                                                                                                   <  1:178557/251‑1 (MQ=255)                                                                                                                        gATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATg                                                                                                             >  2:100320/1‑251 (MQ=255)                                                                                                                            tctGCGCCCTGACGTTCACAAAATGCCTCTATCTGATAGACGTGCAACCGGAGTCATACTGAATTTTCATCATCTGGCTTCCTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAAAGGTGAATATACTGCTGCGGCGGAAAATGAAAACTTTGAAACCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGAGCGATGat                                                                                                           >  2:379269/1‑248 (MQ=255)                                                                                                                             ctGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCCAGAGCGTCGATATCGACCGTGCGATGAgcgc                                                                                                        >  1:50904/1‑251 (MQ=255)                                                                                                                                                                  gACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCActc                                                                   <  2:6020/251‑1 (MQ=255)                                                                                                                                                                                   aTAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCACTCTCTTCTCCGGCTAAACg                                                  <  1:154873/251‑1 (MQ=255)                                                                                                                                                                                    tAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCACTCTCTTCTCCGGCTAAACGt                                                 >  2:136431/1‑251 (MQ=255)                                                                                                                                                                                               catcatCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCACTCTCTTCTCCGGCTAAACGTAAAGCGGTACt                                      <  1:221182/251‑1 (MQ=255)                                                                                                                                                                                                                      tAAAGCGTTAAGTCTCGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCACTCTCTTCTCCGGCTAAACGTAAAGCGGTACTGAATAAACTCGCCGATTTAATgg               >  1:149732/1‑251 (MQ=255)                                                                                                                                                                                                                                     cGCCATTGAAAACTGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCACTCTCTTCTCCGGCTAAACGTAAAGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGa  >  2:96222/1‑249 (MQ=255)                                                                                                                                                                                                                                      gCCATTGAAAACTGCTTAATTATTAACGGTGAATCTACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCACTCTCTTCTCCGGCTAAACGTAAAGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGa  >  1:144826/1‑248 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            CCGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAAGTGTATATAAAGAATGTCGCCAGAAATAAACGGGCATACGGCCCGGGGATCTCTGCGCCCTGACGTTCACAAACTGCATATATCTGATAGACGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCACTCTCTTCTCCGGCTAAACGTAAAGCGGTACTGAATAAACTCGCCGATTTAATGGAAGCCCACGCCGA  >  NC_000913/1362562‑1363038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑