Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,357,473	C→T	V66I (GTA→ATA)	ymjA ←	DUF2543 domain‑containing protein YmjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,357,473	0	C	T	100.0%	101.1 / NA	32	V66I (GTA→ATA)	ymjA	DUF2543 domain‑containing protein YmjA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/16);  total (16/16)

ATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCG  >  NC_000913/1357259‑1357706                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          aTTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTg                                                                                                                                                                                                       >  1:136361/1‑251 (MQ=255)           aactataactCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCttcttc                                                                                                                                                                                              <  1:300861/250‑1 (MQ=255)                        aGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGATTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTTCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCAt                                                                                                                                                                                >  2:148292/1‑251 (MQ=255)                                   gCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCaa                                                                                                                                                                      >  1:128438/1‑250 (MQ=255)                                     gtagagCAGAGCGGGACGATCGTGAGAAACGCATTAAGGCGCGCTGCAGGGCTTTTCATTGTTATCCAGATGCGGCGTGAACGCTCTAGCCGGACAACGATACTGACCGATCGTCTGCAATTAAACCATTACTCATTGCCCCATTGATTCAAAGACTCTGCGATCTCATCAATTCGTATGGGCTTAATCCCCTCTTCAGCACCCATTTCATGGATTGCTTCTTCCGTGATCTTTTCATTGTTCATCaatcc                                                                                                                                                                   <  2:383215/248‑4 (MQ=255)                                            agagCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGAGACTGACCGAGCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGc                                                                                                                                                            >  1:340090/1‑251 (MQ=255)                                             gagCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCTGCGTGAACGCTTTATCCGGACAACGATTCTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCATCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCa                                                                                                                                                           <  1:244985/251‑1 (MQ=255)                                                    aCGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTg                                                                                                                                                    >  2:384372/1‑251 (MQ=255)                                                     cGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGcttct                                                                                                                                                                                                <  2:274624/206‑1 (MQ=255)                                                     cGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAAATCTGCGCTCTCATCAATGCGTCTGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGcttct                                                                                                                                                                                                >  1:274624/1‑206 (MQ=255)                                                            gagaAACCCATTAAAGCACGCTGCAGTGCGTTTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAagtagt                                                                                                                                            <  2:136361/251‑1 (MQ=255)                                                              gaAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTgg                                                                                                                                          <  1:183517/251‑1 (MQ=255)                                                                aaCCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGc                                                                                                                                        >  2:216726/1‑251 (MQ=255)                                                                aaCCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGc                                                                                                                                        <  2:129475/251‑1 (MQ=255)                                                                     aTTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCg                                                                                                                                   <  1:384372/251‑1 (MQ=255)                                                                         aagcaagcTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCCGCAGCCATTTCATGTTGGGCTTCTTCGCTTACCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGgtgt                                                                                                                               >  2:331526/1‑251 (MQ=255)                                                                                              gttGTTAGACAGATGCAGCGTGGACGCGTTATCCGGACAAAGATCCTGACCGGTCGGCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACACTGCGATCTCATCAATGCGTATGGGATTAATCCCCCCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTTATCACTTCATTGTTCATCAAAAGGGTGAGCAGTAACTGGAAGTAGTGGGACAGCGGTGTGCGTTCAGCCTCTGCGACAgg                                                                                                          >  2:228741/2‑251 (MQ=255)                                                                                                        aGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCAt                                                                                                >  2:392505/1‑251 (MQ=255)                                                                                                                   gAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTa                                                                                     <  2:128438/251‑1 (MQ=255)                                                                                                                                   cAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCa                                                                     <  2:306728/251‑1 (MQ=255)                                                                                                                                                 gATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCgg                                                       <  1:392505/251‑1 (MQ=255)                                                                                                                                                         gCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCTTATGGGATTAAGCCCCGCTTCAGCAGCAATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCAACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGatat                                                  <  1:133816/248‑1 (MQ=255)                                                                                                                                                                aaTCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCat                                        >  2:120100/1‑251 (MQ=255)                                                                                                                                                                     ttactcattacCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTAttt                                   >  1:154143/1‑251 (MQ=255)                                                                                                                                                                      tactcattacCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTg                                  >  1:126450/1‑251 (MQ=255)                                                                                                                                                                             tacCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGCTCTCTTCATTGtt                                                                                                                                                                            >  1:38460/1‑106 (MQ=255)                                                                                                                                                                             tacCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCGGCAGCAGCCATTTCATGTTGGGCTGCTGCGCTGAGCTCTTCATTGtt                                                                                                                                                                            <  2:38460/106‑1 (MQ=255)                                                                                                                                                                              acCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTGCCCCTgg                           <  2:137973/250‑1 (MQ=255)                                                                                                                                                                                cccATTGATTCACAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGAAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCCTTATTTGCCCCTGGGGt                        >  2:178818/1‑251 (MQ=255)                                                                                                                                                                                cccATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCa                                                                                                                        >  2:123705/1‑155 (MQ=255)                                                                                                                                                                                cccATTGATTCAAAAACTCTGCGATCTCATCAATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCa                                                                                                                        <  1:123705/155‑1 (MQ=255)                                                                                                                                                                                                           catcaATGCGTATGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCg  <  2:126450/246‑1 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          ATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTGATTCAAAAACTCTGCGATCTCATCAATGCGTACGGGATTAATCCCCGCTTCAGCAGCCATTTCATGTTGGGCTTCTTCGCTGATCTCTTCATTGTTCATCAAACGGGTGAGCAGTAACTGGAAGTAGTGGGCCAGCGGTGTGCGTTCAGCCTCTGCGACAGGCTCTGCGCATTCGGTCGCGTACTCATCGGCAATATCAAAATATTTTAGCGGGATATCGTGGTTCATTATTTGCCCCTGGGGTTAATGCGCTAAGCATAAGGGCG  >  NC_000913/1357259‑1357706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑