Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,355,904	C→T	D403N (GAT→AAT)	sapA ←	putative periplasmic binding protein SapA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,355,904	0	C	T	100.0%	113.1 / NA	35	D403N (GAT→AAT)	sapA	putative periplasmic binding protein SapA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/15);  total (20/15)

TGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGG  >  NC_000913/1355663‑1356122                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                            tgctgcGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCCCTTTTACGCCAACCTGCGCCATATTCGCCTGAAt                                                                                                                                                                                                                    >  2:135881/1‑251 (MQ=255)  gctgcGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGaa                                                                                                                                                                                                                     <  1:352339/249‑1 (MQ=255)    tgcGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATc                                                                                                                                                                                                                   >  2:122324/1‑249 (MQ=255)        aagagcgagccttaagcaccaCGCTGTCGAATTTCGGTTCGCACCAGAGGGCGTGATTGGTCTGCGACTGCATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACAGCCTGGGTCGTTACTGTCCGTAGCCCAACCGGATAACGTCAGATCATGGCTCATATACATTAACCACGCCTCCTGAAAGCGGCCTTCGACCAGCACAATAACCACTTTAACGCCAAACTGAGCAATATTCGCATGAATCAGTTCg                                                                                                                                                                                                             >  2:46954/21‑251 (MQ=255)        aaGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCg                                                                                                                                                                                                             <  1:7048/251‑1 (MQ=255)         agagagCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCg‑g                                                                                                                                                                                                           >  1:358096/1‑251 (MQ=255)                        cAATACACTATCGCATTTCGGATCGCACCAGAAGGCGAGGATGGTCTGCGCATGCATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTAGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACAGCGCCTCCTCAAAGCGAACTTCTACCGGCCCAATCACCACTTTTACGCCAACCTGCGCCATATTCACCTGAATCAATTCG‑GCAGTTTTCAGTGGAc                                                                                                                                                                                            >  2:385336/1‑251 (MQ=255)                              gCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGt                                                                                                                                                                                      <  1:116338/251‑1 (MQ=255)                                    gAATTTCGGATCGCGCCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACg                                                                                                                                                                                <  2:186636/251‑1 (MQ=255)                                               cGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGGCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCATCCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACgt                                                                                                                                                                     <  2:320917/251‑1 (MQ=255)                                                              ggTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGt                                                                                                                                                      >  2:143119/1‑251 (MQ=255)                                                                       gCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGtt                                                                                                                                             <  1:31272/251‑1 (MQ=255)                                                                               atTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTc                                                                                                                                     >  1:98897/1‑251 (MQ=255)                                                                                   ccGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGc                                                                                                                                 >  1:51461/1‑251 (MQ=255)                                                                                              aGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACtt                                                                                                                      >  1:102080/1‑251 (MQ=255)                                                                                                tAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTc                                                                                                                     >  1:312048/1‑250 (MQ=255)                                                                                                  acggaaggAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAAc                                                                                                                  <  2:100600/251‑1 (MQ=255)                                                                                                     gacggaAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGt                                                                                                               >  1:21149/1‑251 (MQ=255)                                                                                                      acggaAGAAACGGTCCGGGTCGTTGCTGTCCGTCGCCCAACCGGATAACGTCATATCATGGCTCATATCCATCAACCGCGCCTCCGGAAAGCGACCTTCTACCGGCACAATCCCCCCTTTTACGCCAGCCTTCTCCATATTCGCCTGAATCAGTGCG‑GCAGTTTTCAGTGGACGGGGTTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAATTTTTCCAGCCCCCACGACGTCAAcgttt                                                                                                              <  2:81958/251‑5 (MQ=255)                                                                                                               aCTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGAtt                                                                                                     >  1:270016/1‑251 (MQ=255)                                                                                                                cTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGAttt                                                                                                    >  1:191431/1‑251 (MQ=255)                                                                                                                         tCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGAtt                                                                                           >  1:289938/1‑251 (MQ=255)                                                                                                                                 gTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAg                                                                                   <  2:191431/251‑1 (MQ=255)                                                                                                                                       cGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTGCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGTATTGTATTCAGTAAttt                                                                             <  1:161174/251‑1 (MQ=255)                                                                                                                                          ccAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAg                                                                          <  1:96117/251‑1 (MQ=255)                                                                                                                                               cGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACt                                                                                                                                                                                           <  1:182471/133‑1 (MQ=255)                                                                                                                                               cGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACt                                                                                                                                                                                           >  2:182471/1‑133 (MQ=255)                                                                                                                                                ggATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGt                                                                    >  2:232842/1‑251 (MQ=255)                                                                                                                                                                   cTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGGTAG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCACACGAggg                                                 >  1:151059/1‑250 (MQ=255)                                                                                                                                                                          ccATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGGCTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCgg                                           <  2:98897/250‑1 (MQ=255)                                                                                                                                                                                 ccGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAAt                                     >  2:46496/1‑249 (MQ=255)                                                                                                                                                                                    cgcCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAgg                                >  2:34572/1‑251 (MQ=255)                                                                                                                                                                                               accgtccctCTACCGGCACAATTGCCGCTTTTACGCCTGCCTGCGCCATATTCGCCTAGATCAGTTCGCGC‑GTTTTCAGTGTTCTGGTGTTCCACGCCGGCGATCTTGTGGGCACCCACCGTTACAGCGTTAAATTTTCCAGCCCCAATCACTTCAACGCTTCGCTCGCTTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGACGCGCGCGGTAAAATAGAGGCCGCCGTTTCa                     <  2:123410/243‑1 (MQ=255)                                                                                                                                                                                                 gggccTTCTACCGGCCCAATCACCCCTTTTACGCCACCCTGCGCCATATTCTCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTTTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGATTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAAGAGAGGCCGCCGTTTCAGc                   <  2:376512/248‑1 (MQ=255)                                                                                                                                                                                                                  aaTCACCACTTTTACGCCAACCTGCGCCATATTCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATgg  >  2:382467/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                            TGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCG‑GCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGG  >  NC_000913/1355663‑1356122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑