Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,353,758	T→A	D254V (GAT→GTT)	sapC ←	putrescine ABC exporter membrane protein SapC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,353,758	0	T	A	91.9%	99.9 / NA	37	D254V (GAT→GTT)	sapC	putrescine ABC exporter membrane protein SapC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (19/15);  minor base C (3/0);  total (22/15)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCGGATTTCACCTTCGGTTAAGGTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑ATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCAT  >  NC_000913/1353519‑1354002                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     ccGCGGATTTCACCTTCGGTTAAGGTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATc                                                                                                                                                                                                                                           <  2:173181/251‑1 (MQ=255)         ttCACCTTCGGTTAAGGTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGGCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTcccc                                                                                                                                                                                                                                    <  2:144719/250‑1 (MQ=255)                     tAAGGTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑ACCACCGAGCATCGCTCCCCATTCAGGCGAggg                                                                                                                                                                                                                       >  2:6129/1‑251 (MQ=255)                     tAAGGTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTACACGGGGCGACATAAATCAGTTCCAGCGC‑AATACCGAGCATCGCTACCCATTCAGGCGAggg                                                                                                                                                                                                                       >  2:198659/1‑251 (MQ=255)                          tCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑ACCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGtt                                                                                                                                                                                                                  >  2:260122/1‑251 (MQ=255)                                  aCCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCg                                                                                                                                                                                                          >  1:180188/1‑251 (MQ=255)                                    ccGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCgag                                                                                                                                                                                                        <  2:352576/251‑1 (MQ=255)                                        tCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGCGTTGTGCGCCg                                                                                                                                                                                                          >  2:282330/1‑245 (MQ=255)                                                  tAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGccc                                                                                                                                                                                           <  2:82906/250‑1 (MQ=255)                                                   aaCCCACTCATCACCGGTTTTAAATTCCATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGccc                                                                                                                                                                                           >  1:340198/1‑249 (MQ=255)                                                                         aaTTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCACAGCGCGGCGATATCGAGAATTGcc                                                                                                                                                                   >  1:224624/1‑251 (MQ=255)                                                                                    cAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGCGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTgc                                                                                                                                                        >  1:317727/1‑251 (MQ=255)                                                                                    cAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTgc                                                                                                                                                        >  1:299600/1‑251 (MQ=255)                                                                                        ttACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑ACCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCgg                                                                                                                                                    >  2:242492/1‑251 (MQ=255)                                                                                                   gAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGt                                                                                                                                         >  1:61611/1‑251 (MQ=255)                                                                                                    aGTAATGGAATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTg                                                                                                                                        >  2:22523/1‑251 (MQ=255)                                                                                                            cATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGccc                                                                                                                                >  2:250856/1‑251 (MQ=255)                                                                                                            cATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGAGACATAAAGCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGccc                                                                                                                                <  1:156004/251‑1 (MQ=255)                                                                                                                        cccGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATggt                                                                                                                    >  1:319956/1‑249 (MQ=255)                                                                                                                                   cGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATGGCCGCCCCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAg                                                                                                         <  2:52997/251‑1 (MQ=255)                                                                                                                                    gCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCAAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGCTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGCGATGTTTGGCCTCACAGc                                                                                                        >  1:395186/1‑251 (MQ=255)                                                                                                                                        gacgaACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAAc                                                                                                    >  1:253286/1‑251 (MQ=255)                                                                                                                                                 cATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCagagcat                                                                                            >  2:336375/1‑247 (MQ=255)                                                                                                                                                       aaaNGAGGTTAACCAACAACACTCTAATCATAATTGCCGCACCTTGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGc                                                                                     <  2:367782/250‑1 (MQ=255)                                                                                                                                                                tAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATCTTCAGCGTTGATgcg                                                                            >  1:90662/1‑251 (MQ=255)                                                                                                                                                                    caacaaCACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGGTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTggtgggcc                                                                          >  2:347576/1‑242 (MQ=255)                                                                                                                                                                           acGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATGCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACg                                                                 <  2:90662/251‑1 (MQ=255)                                                                                                                                                                            cGCTAATCATAATTGCCGCACCTGGAAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGNCgg                                                                >  2:353819/1‑251 (MQ=255)                                                                                                                                                                                                   ggCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCttttt                                          <  1:307380/250‑1 (MQ=255)                                                                                                                                                                                                     cagcaTGACAGTATAAGGGGCCCCATAAATCAGTTCCATCGA‑AACACCGCGCCTCGCCCCACATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAACCCAGCGCGGCGATATCGAGAATCGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGACCGGCGGTGCACTTTGGCATATCAGCAAACCCGAGAATATTCCGAGTTGATGCGCCATCCCGACGggcggcaacaacataatctttctac                                        >  2:188978/1‑231 (MQ=255)                                                                                                                                                                                                         atgaacgtcccccgggCGACATAAATCCATTCCA‑CGCCAACACCCAGCATCGCTCCCCATTCAGGCCAGGGGAGTTGGGGGCCGAGGTCGGGAAAGCCCAGCGCGGGGATAACGAGAATTGCCATCGACAGGGCGCGGGTGATCTCGGTTGCCAGCCCGGCGGTGAAGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTc                                   <  2:180188/238‑1 (MQ=255)                                                                                                                                                                                                           gACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCg                                  <  1:120157/250‑1 (MQ=255)                                                                                                                                                                                                           gACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGAGGCGATAACGTACTCTTTTTCCAGTTCGt                                 >  1:199276/1‑251 (MQ=255)                                                                                                                                                                                                                  caccgggCTACATAAATCAGTTCCAGCGC‑AACACCAAGCATCGCTCCCCATTCAGGCGAGGTGAGTTGTGCGCCGAGATCGAGAAAGCCCAGAGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGAGGCCAGCCCGGCGGTGATGTTTGGCATCGCAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCAc                          <  2:171039/247‑1 (MQ=255)                                                                                                                                                                                                                       ggCNACATAAATCAGTTCCAGCGC‑AACACAGAGACTCGCGCACCATTCAGGCGAGGGGAGTTGTGCGCCGAGACCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACAATGc                     <  2:359722/251‑1 (MQ=255)                                                                                                                                                                                                                                        ccAGCGC‑AACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTAc     <  1:6129/250‑1 (MQ=255)                                                                                                                                                                                                                                          cgcgc‑AACACCGAACATCGCTCACCATTCAGGCGAGGGGAGTTGTGCGCCGAGACCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGTTGATCTCGGTGACCAGCCCGGCGGTGATGTGTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCac  <  2:156800/250‑2 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     CCGCGGATTTCACCTTCGGTTAAGGTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGC‑ATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCAT  >  NC_000913/1353519‑1354002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑