Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,352,193	G→A	P148S (CCG→TCG)	sapF ←	putrescine ABC exporter ATP binding protein SapF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,352,193	0	G	A	100.0%	110.6 / NA	33	P148S (CCG→TCG)	sapF	putrescine ABC exporter ATP binding protein SapF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/21);  total (12/21)

CCTGATGCATCACCAGCACCTGGTCGCTAATGTGCTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGG  >  NC_000913/1351956‑1352435                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   ccTGATGCATCACCAGCACCTGGTCGCTAATGTGCTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACg                                                                                                                                                                                                                                       <  2:351701/251‑1 (MQ=255)    gATGCATCACCAGCACCTGGTCGCTAATGTGCTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGa                                                                                                                                                                                                                                    <  2:91010/251‑1 (MQ=255)      tGCATCACCAGCACCTGGTCGCTAATGTGCTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATc                                                                                                                                                                                                                                  >  1:184309/1‑251 (MQ=255)      tGCATCACCAGCACCTGGTCGATAATGTGCTTCATCATTCCGATATGCTGGGCAACATAAATATACGACATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCCCTAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGCCGCTGTTTTTGTACGGGCGCCAACATATGCGAATAGCAACTGACGTGATc                                                                                                                                                                                                                                  >  2:216893/1‑251 (MQ=255)                     gggTCGCTAATGTGCTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCAGCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCATCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAAc                                                                                                                                                                                                                   <  2:330544/250‑1 (MQ=255)                          gCTACTGTGCTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATACTGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCACCCATAc                                                                                                                                                                                                              >  2:311977/1‑251 (MQ=255)                                gtgCTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTTAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATc                                                                                                                                                                                                         <  2:394784/250‑1 (MQ=255)                                   cTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGttt                                                                                                                                                                                                     >  1:6002/1‑251 (MQ=255)                                             ccGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTg                                                                                                                                                                                           <  1:184828/251‑1 (MQ=255)                                              cGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGt                                                                                                                                                                                          >  2:179082/1‑251 (MQ=255)                                                                       cGAAATGCCCTGTTTTTCCTGGAATTCCAGCATCAGAGTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGTGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGTCCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCAGCGTTTCAATAATCTGTTGGCGACGCTGTTCCGGGTCCAGGt                                                                                                                                                                 <  2:192932/251‑1 (MQ=255)                                                                              cccTTTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGtt                                                                                                                                                          <  2:89184/251‑1 (MQ=255)                                                                                     tttCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGgcg                                                                                                                                                    <  2:109494/250‑1 (MQ=255)                                                                                        ccTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGt                                                                                                                                                <  1:202394/251‑1 (MQ=255)                                                                                                tCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAAGAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTGCAATAAGCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAAt                                                                                                                                         <  1:379022/250‑1 (MQ=255)                                                                                                 ccAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCaata                                                                                                                                                                                                >  1:299656/1‑194 (MQ=255)                                                                                                 ccAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCaata                                                                                                                                                                                                <  2:299656/194‑1 (MQ=255)                                                                                                 ccAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATc                                                                                                                                        <  1:6444/250‑1 (MQ=255)                                                                                                             tAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCg                                                                                                                             >  1:268584/1‑249 (MQ=255)                                                                                                                      gCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGa                                                                                                                  <  2:345990/251‑1 (MQ=255)                                                                                                                                cGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTc                                                                                                         <  2:267129/250‑1 (MQ=255)                                                                                                                                  aCATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATGTCAGCGCGCGCGCCAGGCCCAGACGCCGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGAACCGGTAGCAGCCCAACCATAAGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGAGCAGTGGAAAATCCAGAATTTGCGAGATGCGTTGACGGGGATTCAAc                                                                                                      <  1:10586/251‑1 (MQ=255)                                                                                                                                                  gAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCt                                                                                      >  2:77812/1‑251 (MQ=255)                                                                                                                                                             cGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAg                                                                                                                                                       <  2:291241/175‑1 (MQ=255)                                                                                                                                                             cGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAg                                                                                                                                                       >  1:291241/1‑175 (MQ=255)                                                                                                                                                                           tttGGTCGCAATATCAGCGCGCTCGCCATATCCAGACCCTTGTTTTGTCCGGGTGCCAACATTTTCTAATAGTAACTGACGTGATCCGGTAGCAGCCCACCCATACGCATCGTTTCAATAATCTGTTTGCGACGTTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGTAAAATCCAGAATTTGCGAGATACGTTTACTTTGATTCAACGAGGACGAAGGATCCCTATAAATCATGCTACTACGCTGAc                                                              <  2:217929/250‑1 (MQ=255)                                                                                                                                                                           tttGGGCGCAATATCAGCACGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCCTCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCATGTCGGTGTTCAGGCGCAGTGGAAAAGCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCAGGCGAATACGCTGa                                                               <  1:22885/249‑1 (MQ=255)                                                                                                                                                                               ggCGCAATAGCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCACCCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGTGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCgg                                                         <  2:342868/251‑1 (MQ=255)                                                                                                                                                                                    aaTATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCggaagg                                                     >  2:232268/1‑250 (MQ=255)                                                                                                                                                                                      tatCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGNaat                                                  >  2:362572/1‑251 (MQ=255)                                                                                                                                                                                                                   gTTTTTGTCCGGGTGCCAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGtc                      <  2:299620/250‑1 (MQ=255)                                                                                                                                                                                                                                   cAACATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTcgccgc     >  1:109260/1‑251 (MQ=255)                                                                                                                                                                                                                                      cATATGCGAATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTgg  <  1:362572/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   CCTGATGCATCACCAGCACCTGGTCGCTAATGTGCTTCATCATTCCGATATGCTGGGTAACATAAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGG  >  NC_000913/1351956‑1352435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑