Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,163,609	G→A	intergenic (‑339/‑30)	trmA ← / → btuB	tRNA m(5)U54 methyltransferase/cobalamin outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,163,609	0	G	A	100.0%	119.0 / NA	39	intergenic (‑339/‑30)	trmA/btuB	tRNA m(5)U54 methyltransferase/cobalamin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/16);  total (23/16)

CTGGACAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGTTGTGACCCGTCAGGATATCGACCGCTGGCAGTCGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGC  >  NC_000913/4163362‑4163851                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   cTGGACAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAATCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACAt                                                                                                                                                                                                                                                 >  2:82721/1‑251 (MQ=255) cTGGACAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACAt                                                                                                                                                                                                                                                 >  2:25853/1‑251 (MQ=255)      cAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGGCCTGTGAGTTAATAGGGAATCCAGTGCGAAGCTGGAGCTGACGCGCCGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGACAACGTCGCATCTGGTTCTCATCATCGCGTAATATTCCTGAATCCTGCGACATCcttc                                                                                                                                                                                                                                            <  2:65620/251‑1 (MQ=255)       aGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCcttct                                                                                                                                                                                                                                           >  1:19483/1‑251 (MQ=255)                    aaCCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATg                                                                                                                                                                                                                              >  1:167063/1‑251 (MQ=255)                    aaCCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATg                                                                                                                                                                                                                              <  1:355646/251‑1 (MQ=255)                           aGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTAc                                                                                                                                                                                                                        <  1:217074/250‑1 (MQ=255)                                   cTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGATGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTCGCAAGTCATCATCTCTTAGTATCTTCGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTa                                                                                                                                                                                                                >  1:37105/1‑250 (MQ=255)                                        cGGTCCTGTGCGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTaaaaa                                                                                                                                                                                                            >  2:240632/1‑249 (MQ=255)                                            ccTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTc                                                                                                                                                                                                      >  2:131273/1‑251 (MQ=255)                                              tgtgAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCgc                                                                                                                                                                                                    >  2:294567/1‑251 (MQ=255)                                                      aaTAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACg                                                                                                                                                                                            <  1:2845/251‑1 (MQ=255)                                                                  aGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTc                                                                                                                                                                                >  2:137415/1‑251 (MQ=255)                                                                      cGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACgg                                                                                                                                                                            >  1:79045/1‑251 (MQ=255)                                                                       gAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGc                                                                                                                                                                           <  2:148521/251‑1 (MQ=255)                                                                          tCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCTATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACTTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATTAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCAtt                                                                                                                                                                        >  1:248279/1‑251 (MQ=255)                                                                                       cgcAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGca                                                                                                                                                           <  1:25853/251‑1 (MQ=255)                                                                                                                      ttacgCGGACACGGCCATTCGGTGGTAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAATGCCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCGTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACCGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTcgtcgt                                                                                                                              <  2:245459/245‑1 (MQ=255)                                                                                                                             gACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTaa                                                                                                                     <  1:240632/251‑1 (MQ=255)                                                                                                                                               gAAGTCATCATCTCTTATTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAAGATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTTCTGCTAACCGTTTTGAACTGCcgcg                                                                                                   <  2:248279/251‑1 (MQ=255)                                                                                                                                                         tctcTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACtgtg                                                                                         >  1:398503/1‑251 (MQ=255)                                                                                                                                                            ctTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACtgt                                                                                          <  1:329487/247‑1 (MQ=255)                                                                                                                                                                gTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCAt                                                                                                                                                                         <  1:101496/164‑1 (MQ=255)                                                                                                                                                                gTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCAt                                                                                                                                                                         >  2:101496/1‑164 (MQ=255)                                                                                                                                                                    cTTAGATCCCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCaccacc                                                                              >  2:220276/1‑249 (MQ=255)                                                                                                                                                                          tACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGt                                                                        >  1:70989/1‑251 (MQ=255)                                                                                                                                                                                 ccAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGTTGTGAcc                                                                 >  2:372861/1‑251 (MQ=255)                                                                                                                                                                                        cGAAGACCTGCCGGCAAACGTCGCATCTGGTTCTCATCCTCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCCACCACCGTTGTGACCCGTCAgg                                                          >  2:42783/1‑251 (MQ=255)                                                                                                                                                                                               cTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGTTGTGACCCGTCAGGatat                                                      <  2:203073/248‑1 (MQ=255)                                                                                                                                                                                               cTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGTTGTGACCCGTCAGGatat                                                      >  1:203073/1‑248 (MQ=255)                                                                                                                                                                                                    ggCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGTTGTGACCCGTCAGGATATCGACCGCt                                              <  2:79045/251‑1 (MQ=255)                                                                                                                                                                                                                  cTGGGTCTCCTCATCGCGAAATATTGATGAAACCTGCGACATCCTTCTCCTATTGTGGATGCTTTACAATGATTACAAACGCTTCGCTGCTGACGTCGTGCTCCGTCAAGGCATTTTCTGCTTGGGCACCGGATACCAGCCCTGACACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGATTCCACCAACCACCGTTGTGTCCCGTCAGGATATCGACCGCTGGCAGTCGACATCg                                >  2:214462/1‑251 (MQ=255)                                                                                                                                                                                                                        ctcATCATCGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGTTGTGACCCGTCCGGATATCGACCGCTGGCAGTCGACCTCGGTCAat                          >  2:167692/1‑251 (MQ=255)                                                                                                                                                                                                                           atcatcGCGTAATATTGATGAAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGTTGTGACCCGTCAGGATATCGACCGCTGGCAGTCGACCTCGGTCAatgat                       >  1:386687/1‑251 (MQ=255)                                                                                                                                                                                                                                           gatgaAACCTTCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGTTGTGACCCGTCAGGATATCGGCCGCTTGCAGTCGACCTCGGTCAATGATGTGCTGCTCCGTCtt        <  2:24351/250‑1 (MQ=255)                                                                                                                                                                                                                                           gatgaAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGTTGTGACCCGTCAGGATATCGACCGCTGGCAGTCGACCTCGGGCAATGATGTGCTGCGCCGTCTTc       >  2:274442/1‑251 (MQ=255)                                                                                                                                                                                                                                            attaaaCCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGTTGTGACCCGTCAGGATATCGACCGCTGGCAGTCGACCTCGGTCAATGATGTGCTGCGCCGTCTTcc      <  2:386687/248‑1 (MQ=255)                                                                                                                                                                                                                                              gaAACCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATGTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGTTGTGACCCGTCGGGATATCGACCGCTGGCAGTCGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCg     <  1:359702/250‑1 (MQ=255)                                                                                                                                                                                                                                                aaCCTGCGACATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGTTGTGACCCGTCAGGATATCGACCGCTGGCAGTCGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGc  >  1:300914/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   CTGGACAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGTTGTGACCCGTCAGGATATCGACCGCTGGCAGTCGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGC  >  NC_000913/4163362‑4163851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑