Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,132,970 G→A G119S (GGC→AGC)  metF → 5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,132,9700GA95.5% 139.1 / NA 44G119S (GGC→AGC) metF5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (24/18);  minor base C (2/0);  total (26/18)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTGGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGT  >  NC_000913/4132725‑4133211
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 
tGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGAt                                                                                                                                                                                                                                              >  2:12028/1‑251 (MQ=255)
                   cTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTgccgcc                                                                                                                                                                                                                                      >  2:345817/1‑240 (MQ=255)
                   cTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTgccgcc                                                                                                                                                                                                                                      <  1:345817/240‑1 (MQ=255)
                     ccATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTa                                                                                                                                                                                                                           >  2:96590/1‑249 (MQ=255)
                     ccATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTa                                                                                                                                                                                                                           >  1:313514/1‑249 (MQ=255)
                             cgCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTTGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGaaa                                                                                                                                                                                                                  <  2:225700/250‑1 (MQ=255)
                                   agcagcCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGc                                                                                                                                                                                                           <  1:12028/251‑1 (MQ=255)
                                            gaactgAAGGTTGTATCTGTGACCTATGGCGCGAAGTCCGGCGAGGGCGACCGTAAGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGGGCTGCGCACCATTGCACGCGACTACTGGTATAACGGGATTCGTCATAGCGTGGCGCTGCGTAGCGATCTGCTGCCGGGCAGCGGTAAGCGAGAAATGTATGCTGCTGACCt                                                                                                                                                                                                  >  2:143108/6‑251 (MQ=255)
                                               ccGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCCCCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGt                                                                                                                                                                                               <  2:99266/251‑1 (MQ=255)
                                                     tttGTATCGGTGACCTATGGCGCGAACTCCGGCGCGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTCGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTGCTCACCTAGTGACGCt                                                                                                                                                                                         >  2:17218/1‑251 (MQ=255)
                                                          aTCGGTGACCTATGGCGCGAACTCCGGCGCGCGCGACCGTACGCACAGCATTATTCAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACTCGACTACTGGAATAACGGTATTCGTCATCTCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGACATGTCTGCTTCTGACCTGGTGACGCTGTTa                                                                                                                                                                                     >  2:104927/1‑250 (MQ=255)
                                                          aTCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTaa                                                                                                                                                                                    <  2:379368/251‑1 (MQ=255)
                                                            cGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTaaaa                                                                                                                                                                                  <  2:313514/251‑1 (MQ=255)
                                                               tGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAaaga                                                                                                                                                                                >  1:15554/1‑250 (MQ=255)
                                                                  ccTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTg                                                                                                                                                                            >  2:330036/1‑251 (MQ=255)
                                                                                 ccGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGata                                                                                                                                                               <  1:362332/249‑1 (MQ=255)
                                                                                       aGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTg                                                                                                                                                       <  2:15554/251‑1 (MQ=255)
                                                                                           cgACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGACTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAACAGAAGAGGCAGATTTCGATATCTCCGTggcgg                                                                                                                                                   >  2:67166/1‑251 (MQ=255)
                                                                                                     cacaGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGACGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCgg                                                                                                                                          >  1:200101/1‑250 (MQ=255)
                                                                                                           attattAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCAAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTc                                                                                                                                    >  1:69311/1‑250 (MQ=255)
                                                                                                              attaAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTCCTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCCTATCGTGGCGCTGCGTAGCGATCTGCCGCCGTGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGCCGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGAGGCGTATCCGGAAGTTCcccc                                                                                                                                >  2:157131/1‑251 (MQ=255)
                                                                                                                        ttAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGACGTTCACCCGGAAGCaaa                                                                                                                       >  2:129602/1‑250 (MQ=255)
                                                                                                                              aTCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCt                                                                                                                <  1:350563/251‑1 (MQ=255)
                                                                                                                                  cACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCa                                                                                                              <  1:152659/249‑1 (MQ=255)
                                                                                                                                  cACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCa                                                                                                              >  2:152659/1‑249 (MQ=255)
                                                                                                                                   aCTGGTCTGGAAGCGGCACCGCATCTTGCTTGCAGTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTTCCGCCGGGAAGTGGTAAGCCAGAAATGTATTCTTCTTACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTTGATATCTCCGTGGCGCCGTATCCGGATGTTCACCCGGAAGCAAAAAGCGCTCAGGc                                                                                                           <  2:150423/251‑1 (MQ=255)
                                                                                                                                      ggTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGa                                                                                                        <  1:131504/251‑1 (MQ=255)
                                                                                                                                           ggAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGc                                                                                                   >  1:397335/1‑251 (MQ=255)
                                                                                                                                            gAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAaagacg                                                                                                                                                                              >  2:221001/1‑173 (MQ=255)
                                                                                                                                            gAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAaagaag                                                                                                                                                                              <  1:221001/175‑1 (MQ=255)
                                                                                                                                                 ggCACCGCATCTTAATTGCATTGATGCGACGCACGACGAGATGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCAGTGGCGGCGTATCCGGAAGCTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATc                                                                                             >  1:122772/1‑251 (MQ=255)
                                                                                                                                                      cGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCCCCATTTCACGCGACTACTGGAATAACGGTATTCGTCATAGCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTATCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCGTAATCTGaaa                                                                                        <  1:210852/251‑1 (MQ=255)
                                                                                                                                                        cATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATATCGATATCTCCGTGGCGGCGTATCCGGAAGGTCACCCGGAAGCAAAAAGCGCTAAGGCGGTTTTGCTTAATCTGAAACg                                                                                      >  2:116353/1‑251 (MQ=255)
                                                                                                                                                                 ggCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTgg                                                                              >  2:52241/2‑250 (MQ=255)
                                                                                                                                                                              cGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGACAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGCTTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAAc                                                                >  1:286682/1‑251 (MQ=255)
                                                                                                                                                                               gCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAAcc                                                               >  2:148923/1‑251 (MQ=255)
                                                                                                                                                                               gCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAAcc                                                               >  2:57523/1‑251 (MQ=255)
                                                                                                                                                                                              cACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGt                                                >  1:97403/1‑251 (MQ=255)
                                                                                                                                                                                                        cgcgACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGa                                      <  1:148923/251‑1 (MQ=255)
                                                                                                                                                                                                          cgACTACTGGAATAACGGTATGCGTCATAACGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCAGTCCTGGTGACGCTGTTAAAAGAAGGGGCAGAGTTCGAAATCTCCGTGGCGGCGTATCCGGAAGGTCACCCGGAACCAAAAAGCGCGCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGTATGCCGGAGCAAACCGCGCGATTACTCAGTGCTTAATCGATg                                    <  2:46645/251‑1 (MQ=255)
                                                                                                                                                                                                              tactGGAATAACGGCATTCGTCATATCGTTGCGCTGCGCCGCGATGCGCGGTCCAGAGATGGTACGCCAGAAATGTAGCCTTCTGCCATGGTGCCGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCTGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGATCACGCGGATTTGCTTAATCTGAAACGCAAAGTGGAGGCCGGAAACACCCGCACGCTTTCTCAGTTCTTCTTCgctgn                                   >  2:368746/1‑244 (MQ=255)
                                                                                                                                                                                                                                    atatCGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACTTGCGTTTTCGTGAc          <  1:293721/251‑1 (MQ=255)
                                                                                                                                                                                                                                        cGTGGCGCTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCt      <  2:172338/251‑1 (MQ=255)
                                                                                                                                                                                                                                             cgcTGCGTAGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTgtgt  >  2:232684/1‑250 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 
TGGAGCAGACCCTGTGGAACTCCATCGATCGCCTTAGCAGCCTGAAACCGAAGTTTGTATCGGTGACCTATGGCGCGAACTCCGGCGAGCGCGACCGTACGCACAGCATTATTAAAGGCATTAAAGATCGCACTGGTCTGGAAGCGGCACCGCATCTTACTTGCATTGATGCGACGCCCGACGAGCTGCGCACCATTGCACGCGACTACTGGAATAACGGTATTCGTCATATCGTGGCGCTGCGTGGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGT  >  NC_000913/4132725‑4133211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑