Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,091,821	G→A	Y182Y (TAC→TAT)	frvB ←	putative PTS enzyme IIBC component FrvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,091,821	0	G	A	97.1%	118.6 / NA	35	Y182Y (TAC→TAT)	frvB	putative PTS enzyme IIBC component FrvB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (21/13);  minor base C (1/0);  total (22/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCGGCAAAAAGCTGCGAGTTTGTCGGT  >  NC_000913/4091579‑4092058                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              aTTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGCTAACCGa                                                                                                                                                                                                                                        >  2:200491/1‑250 (MQ=255)       ccGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTc                                                                                                                                                                                                                                 <  1:144485/251‑1 (MQ=255)          agcagcGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAAc                                                                                                                                                                                                                              <  1:394850/251‑1 (MQ=255)             agcGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTAc                                                                                                                                                                                                                           <  1:356318/251‑1 (MQ=255)                      aaTGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTaa                                                                                                                                                                                                                   <  1:268610/250‑1 (MQ=255)                           tttGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAacgacg                                                                                                                                                                                                             >  2:400402/1‑251 (MQ=255)                                            ggCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACata                                                                                                                                                                                            <  2:172162/250‑1 (MQ=255)                                               ggAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAATCCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCTTAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCg                                                                                                                                                                                         >  1:11307/1‑251 (MQ=255)                                               ggAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCg                                                                                                                                                                                         <  1:311084/251‑1 (MQ=255)                                                       aagaCGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATCTCGGTATACgg                                                                                                                                                                                 >  2:219578/1‑251 (MQ=255)                                                                aaGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTAGCGGCAATCGACGAGGCAATATAAGCGCCCAGGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACGACCGAGGTAAACGACGGCGCGCCGTTCGACATACCGGTATACGGCAAACCGaa                                                                                                                                                                        <  1:158762/250‑1 (MQ=255)                                                                     aaTCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGc                                                                                                                                                                    >  1:90074/1‑250 (MQ=255)                                                                           aaGCCCCAGAACCACCGCGACGAGGAATGCCGAACCCGACTGGGTGCGGTGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAATTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCCGTATACGGCAAACCGAACTGCACCAGCa                                                                                                                                                             <  1:180363/250‑1 (MQ=255)                                                                                 cAGCACCCCCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAAcc                                                                                                                                                                           <  2:89813/231‑1 (MQ=255)                                                                                 cAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAAcc                                                                                                                                                                           >  1:89813/1‑231 (MQ=255)                                                                                                  ggAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGAt                                                                                                                                       >  2:196261/1‑250 (MQ=255)                                                                                                     agtccGCACCCGACTGGGTGACTAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATacca                                                                                                                                   <  2:321865/248‑1 (MQ=255)                                                                                                                      gTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACgg                                                                                                                  >  1:110073/1‑251 (MQ=255)                                                                                                                          cGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAgc                                                                                                              >  2:183198/1‑251 (MQ=255)                                                                                                                           gAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAgcg                                                                                                             >  1:157597/1‑251 (MQ=255)                                                                                                                                        tCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGAcgcc                                                                                                >  2:270564/1‑251 (MQ=255)                                                                                                                                             cGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGAcgcccgccc                                                                                           >  1:386543/1‑250 (MQ=255)                                                                                                                                                    tAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGCTGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATCTCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCACGACATCAGGTg                                                                                    >  2:340195/1‑251 (MQ=255)                                                                                                                                                        aaaCCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCACATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCtc                                                                                >  2:42468/1‑251 (MQ=255)                                                                                                                                                             aaaaccgcggagacaaaagcgtgTTTACCGGAAATCGACGAGGAAATAAAAGCGCCCATGAGGGGGACCAAAAAGGTGAAGCAAAAATAACCGATGAATACAACTACCCAGGAAACCGCCGGCCCCCCTTTCGAAATATCGTTATCCGCCAAACCGAACTCCACCAGCATTTTGGAAACCGCCCCCAGTATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCAc                                                                           <  1:196261/229‑1 (MQ=255)                                                                                                                                                                      ggAGCGAAAGCCGGGTTACCGGCAATCGCCGAGGCAATCTAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTAGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCACGTGGCTCATCACGCTACCGCt                                                                  >  2:178408/1‑251 (MQ=255)                                                                                                                                                                                    tttATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATAACGCTACCGCTTTGCACTTCCTGtt                                                    >  1:76408/1‑251 (MQ=255)                                                                                                                                                                                      tctcggaaatgtacgaGGCAAAATACCCGCCCATGATGGGCTTCAAAAAGGTAATGCCAAGATACCCGATGAATTCAACCCCCCATGTAAACGACGCCCCGCTTTCCGACTTATCGGTATCCGGCAAACCGACCGGCCCCAGCATGTGGGCAAGCGCCACAAGAATCCCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGttt                                                   <  1:300201/238‑1 (MQ=255)                                                                                                                                                                                                  acgaGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGc                                                                                                                                                                                <  2:386122/113‑1 (MQ=255)                                                                                                                                                                                                  acgaGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGc                                                                                                                                                                                >  1:386122/1‑113 (MQ=255)                                                                                                                                                                                                          atatAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATc                              >  1:286561/1‑251 (MQ=255)                                                                                                                                                                                                                     cATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGAcgcccgcc                                                                                            >  1:278647/1‑178 (MQ=255)                                                                                                                                                                                                                     cATGATGGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCGGCaaaa                    >  1:253613/1‑250 (MQ=255)                                                                                                                                                                                                                          tgGGGATCATAAAGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCGGCAAAAAGCTGc              >  2:33442/1‑251 (MQ=255)                                                                                                                                                                                                                                      aGGTGAAGCCAAGATAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCGGCAAAAAGCTGCGAGTTTGTCGGt  >  2:239728/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              ATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGCCGATGACAAACGGCAGCGCCGCAGAGACGCCCGCCATCAGGTGGCTCATCACGCTACCGCTTTGCACTTCCTGTTTGCCAAGCTTCACGCCGCTATCTGCGGCAAAAAGCTGCGAGTTTGTCGGT  >  NC_000913/4091579‑4092058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑