Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,065,293	G→A	R158R (CGC→CGT)	yihO ←	putative sulfoquinovose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,065,293	0	G	A	100.0%	78.5 / NA	25	R158R (CGC→CGT)	yihO	putative sulfoquinovose transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/17);  total (8/17)

AAACCAGCAATGGCGGATTCCGAAATATCGCGCAAAATGATTTGAGAATACTGGCTTTATGTCCGGTTGGCGTTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATA  >  NC_000913/4065046‑4065530                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              aaaCCAGCAATGGCGGATTCCGAAATATCGCGCAAAATGATTTGAGAATACTGGCTTTATGTCCGGTTGGCGTTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGACgg                                                                                                                                                                                                                                            <  1:278204/251‑1 (MQ=255)     ctgCAATGGCGGATTACGAAATATCGCGCAAAATGATTTAAGAATACTGTCTCTATGTCCCGCTGGCGTTGTGTCCACGTAACGATCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGACAATGGAGAACAAACGTGCCGCACAGGCATAACCGACGGTGGAGTAAGAAAAAAGCGACTGCAACGGAATACACCCCACGGGACAAACCCATCGCCCTAAGGGTGCACCACCCCGACGGTaac                                                                                                                                                                                                                                        >  2:201781/3‑250 (MQ=255)                 aTTCCGAAATATCGCGCAAAATGATTTGAGAATACTGGCTTTATGTCCGGTTGGCGTTGTGTCCACATAAGGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGACGGTAAGCCGCGAGCTgcg                                                                                                                                                                                                                            <  2:77705/251‑1 (MQ=255)                                   aaaTGATTTGAGAATACTGGCTTTATGTCCGGTTGGCGTTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGACGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGtttt                                                                                                                                                                                                          >  1:201077/1‑251 (MQ=255)                                   aaaTGAATTGAGAATACTGGCTTTATATCCGCTTGGCGTTGTGCCCACACAATGCTCCTTGACGCCTCTGTAGCACAGCATCATAAAAAGAAAGCCGCCAATGCAGAACATAAGCGCCCCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCCCCCTGACGGTAAGCCGCGAGCTTCGCGCGCCCATTCGTGttct                                                                                                                                                                                                          <  2:258864/251‑3 (MQ=255)                                          ttGAGAATATTGGCTTTATGTCCGGTTGGCGTTGTGTCCACATAATGCTCTTTGACGCGCCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCATAAAAAAGCGACTGCAACGGAATAAACGCCACGGGACAAATCAATAGCCCTATGGTTGCACCACCCTGACGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTaa                                                                                                                                                                                                    <  1:352037/250‑1 (MQ=255)                                          ttGAGAATACTGGCTTTATGTCCGGTTGGCGTTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGACGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTaa                                                                                                                                                                                                    <  1:307818/250‑1 (MQ=255)                                            gagaATTCTGGCTTTATGTTCGGTTGGCGTTGTGTACACATAATGCTCTTTGACGCCGCTGTAGCAAAGAATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCACTATGGTTGCACCAGCCTGACGGTAAGCCGCGACCTGCGCGCGCTCATTCGGGTTTTTGGTAAtt                                                                                                                                                                                                  <  1:44634/250‑1 (MQ=255)                                                     ggCTTTATTTCCGGTTGGCGTTGTGTCCACATAATGCTCTTTGACGCCTCTGGAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGACGGTAAGCCGCGAGCTGCGCGCGCTCAGTCGGGTTTTTGGTAATTGCCGGGat                                                                                                                                                                                          <  1:93333/249‑1 (MQ=255)                                                     ggCTTTATGTCCGGTTGGCGTTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGACGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGatc                                                                                                                                                                                         <  1:60893/250‑1 (MQ=255)                                                                 ggTTGGCGTTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGACGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACg                                                                                                                                                                            >  1:255653/1‑251 (MQ=255)                                                                             gtCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGACGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTtcatc                                                                                                                                                                 >  1:83998/1‑250 (MQ=255)                                                                                                          aGCACAGCATCAAAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGTCGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGACGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATGGCCGGGATCATCGCTCCATACGAGCAGTTCATCAGACTGTAGGAAAGTCCAAACATCATAAAc                                                                                                                                   <  2:62063/251‑1 (MQ=255)                                                                                                             acaGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGACGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAgc                                                                                                                                >  1:183589/1‑251 (MQ=255)                                                                                                                    catAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGACGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTgg                                                                                                                         <  1:237435/251‑1 (MQ=255)                                                                                                                                                                        aCGGTTGAGTCAGAAAAACGCGACTGCAACGGAATAAACGCCACGGTACACATCAATAGCCCTATGGTTGCACCACCCTGACGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAACCTGGANCGt                                                                     >  2:354775/1‑251 (MQ=255)                                                                                                                                                                                gTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGACGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTa                                                             <  1:169597/251‑1 (MQ=255)                                                                                                                                                                                                  cAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGACGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTg                                           <  1:192192/251‑1 (MQ=255)                                                                                                                                                                                                           aaaCGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGACGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaa                                   >  1:48302/1‑250 (MQ=255)                                                                                                                                                                                                                 cACGGTACAAATCAATAGCCCTCTGGTTGCACCACCCAGACGGTAAGCCGCGACCTGCGCGCGCTCATCCGGGTTTTTGGTAATTGCCGGGATCATCGCACCCTACGAGCAGTTCATCAGCCTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGCCGTTTTAACCGGCAAACAAACGGCGGTGGCGATAAACTGGAGCGTTGCACTTACCGCCGCCGGAACCGCTGCATataaaataacgg                            >  2:167813/1‑248 (MQ=255)                                                                                                                                                                                                                    ggTACAAATCAATAGCCCTACGGTTGCCCCACCCTGACGGTAAGCCGCGAGCTGCGCTCGCGCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGTAGTGCATCAGACTGTATGACATTCTAAACATCATAACCAGCGTGGTGGCAATCGTCGTTTTAACCGGCACACAAAAGGTGCTGGTGATAAACTGGAGCGTTGCAATTAACGTCGCCGGAACCGCTGCATATAAAATAAAGGGAc                         <  2:138508/251‑1 (MQ=255)                                                                                                                                                                                                                     gTACAAATCAATAGCCCTATGGTTGCACCACCCTGACGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACg                        <  2:144423/251‑1 (MQ=255)                                                                                                                                                                                                                         aaaTCAATAGCCCTATGGTTGCACATCCCCGACGTTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGAACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAGCAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAt                    <  2:317909/251‑1 (MQ=255)                                                                                                                                                                                                                              aaTAGCCCTATGGTTGCACCACCCTGACGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGcc               <  1:243452/251‑1 (MQ=255)                                                                                                                                                                                                                                            tGCACCACCCTGACGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAata  <  1:222582/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              AAACCAGCAATGGCGGATTCCGAAATATCGCGCAAAATGATTTGAGAATACTGGCTTTATGTCCGGTTGGCGTTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATA  >  NC_000913/4065046‑4065530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑