Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,055,224	G→A	L19F (CTT→TTT)	glnG ←	DNA‑binding transcriptional dual regulator NtrC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,055,224	0	G	A	100.0%	218.5 / NA	64	L19F (CTT→TTT)	glnG	DNA‑binding transcriptional dual regulator NtrC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/40);  total (24/40)

TGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCGTATCCTGCAAATGAGGCGGAATGCC  >  NC_000913/4054992‑4055469                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      tgttgATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGaa                                                                                                                                                                                                                                       <  2:208135/249‑1 (MQ=255) tgttgATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGaa                                                                                                                                                                                                                                       <  1:125023/249‑1 (MQ=255)    tgATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATc                                                                                                                                                                                                                                  >  1:364838/1‑251 (MQ=255)      aTAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGt                                                                                                                                                                                                                                <  1:357084/251‑1 (MQ=255)        aGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGCCATAATGAAGACCGGACGCAATAGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTTCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCCAACACCCAACGGATGGAACTATCGTc                                                                                                                                                                                                                               >  1:396319/1‑250 (MQ=255)         ggCGCTGACGGCAGCATCCAGATCGGAATGTGCTGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCAt                                                                                                                                                                                                                             <  2:264384/251‑1 (MQ=255)                aCGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTAc                                                                                                                                                                                                                      >  1:374537/1‑251 (MQ=255)                 cGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTAcc                                                                                                                                                                                                                     >  1:358154/1‑251 (MQ=255)                            aGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATccc                                                                                                                                                                                                          <  1:161426/251‑1 (MQ=255)                             gagcGGAATGTGCGGACATAATGATGCCCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCCCATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCGCTTCTGCGCCGTTCGCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACGCCCAGCGGATGGAACTAGCGTCATCGACTACCCATACTATCCCt                                                                                                                                                                                                         <  2:296519/248‑1 (MQ=255)                                 ggAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATATGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGtt                                                                                                                                                                                                     >  1:19651/1‑251 (MQ=255)                                  gAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTc                                                                                                                                                                                                        <  2:358154/247‑1 (MQ=255)                                         cGGTCATAAGGATGACCGGAAGCATTGGATGGCGCTGTTTCATCTGCTTGAGCAGCGACAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTGTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAAc                                                                                                                                                                                             <  2:6181/251‑1 (MQ=255)                                          ggTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACg                                                                                                                                                                                            <  1:330470/251‑1 (MQ=255)                                                 atgatgACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCtt                                                                                                                                                                                     <  1:174972/251‑1 (MQ=255)                                                  tgatgaCCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACg                                                                                                                                                                                                                                              >  2:277001/1‑193 (MQ=255)                                                  tgatgaCCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACg                                                                                                                                                                                                                                              <  1:277001/193‑1 (MQ=255)                                                   gatgaCCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTa                                                                                                                                                                                   <  1:118519/251‑1 (MQ=255)                                                            aaGCATTGTATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATATGGAAATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGTCTCCAGCGCTTCTGCGCCGTTCTCAAACGTCGTACAGTTTCAACCTGCCCCTGCGAGCGCACGTTCAAACACCCAACGGATTGAACTTTCGTCATCAGCTACCCAAACTATCCCTCGTTGCATAACCGGCTCCTTTGTTTCCTGAt                                                                                                                                                                          <  2:166126/251‑1 (MQ=255)                                                                  tGGATGGCGCTGTTTAATCTGCTTGAGCATCGCCAGCCCGTCCATTCCCGGCATAAGGATATCTGAAAGCAGCACATCCGACGTTTTGCTCGCCAGCGCCTCCAGAACTTCTGCGCCGTTCTCAAACGTCGTACAGGATAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCATCGTTGCATAAACGACACCTTTATTTCCTGATaagc                                                                                                                                                                      >  2:79415/1‑246 (MQ=255)                                                                  tGGATGGCGCTGTTTAAGCTGCTTGAGCAGCGGCAGCCCGTTCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCGTTATTTCCTGATAGGCa                                                                                                                                                                     <  2:258805/250‑1 (MQ=255)                                                                      tGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTa                                                                                                                                                                 >  2:12707/1‑250 (MQ=255)                                                                      tGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTa                                                                                                                                                                 >  1:106698/1‑250 (MQ=255)                                                                         cgcTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAcc                                                                                                                                                             <  1:312798/251‑1 (MQ=255)                                                                               ttAATCTTCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGaa                                                                                                                                                        <  1:120614/250‑1 (MQ=255)                                                                                      gCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCgg                                                                                                                                                   <  1:344803/248‑1 (MQ=255)                                                                                      gCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCgg                                                                                                                                                   <  2:50093/248‑1 (MQ=255)                                                                                      gCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTAt                                                                                                                                                <  1:152686/251‑1 (MQ=255)                                                                                             cagcGCCAGCCCTTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTgg                                                                                                                                         <  2:264068/251‑1 (MQ=255)                                                                                              agcGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGc                                                                                                                                        >  1:357766/1‑251 (MQ=255)                                                                                                ggcCAGCCCGTCCCTTCCCGGCCTACGGATACCTGATAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCACCCCTTCTGCGCCGTTCTCAAAAGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACTCCCAACGGATTGAACTATCGTTTTCGACTACCCAGACTATCCCTCGTTGCATAATCGTCACTTTGACTTCTTTATAGGCAGGTAAACCGACATCTCGGTATGCCCTGGCCa                                                                                                                                      <  2:171887/250‑1 (MQ=255)                                                                                                 gcCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCa                                                                                                                                      >  2:319387/1‑250 (MQ=255)                                                                                                  aaaGCCCGTCCATTCCCGGCATAAGGATATATGAAAGCAGCAAATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTAAGCCGGTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCACACACCCAACGGCTGGAACTATCGTCATCGACTACCCAGACTATCCCTCGCTGCATAACCGTCACCTTTATTTCCTGATAGGCCGGTAAACCGAGAACTCGGTATGACCTTGCCAAc                                                                                                                                    >  2:338856/3‑251 (MQ=255)                                                                                                     gCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTgg                                                                                                                                 <  2:148957/251‑1 (MQ=255)                                                                                                            cATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTc                                                                                                                          >  1:202331/1‑251 (MQ=255)                                                                                                                cccGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAt                                                                                                                       <  2:350892/250‑1 (MQ=255)                                                                                                                        aCGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTg                                                                                                               <  2:202331/250‑1 (MQ=255)                                                                                                                        aCGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGa                                                                                                              <  1:317612/251‑1 (MQ=255)                                                                                                                          ggATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAAt                                                                                                            >  2:197634/1‑251 (MQ=255)                                                                                                                          ggATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAAt                                                                                                            <  1:293045/251‑1 (MQ=255)                                                                                                                                  gNAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGat                                                                                                      <  2:357766/249‑1 (MQ=255)                                                                                                                                  gAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGa                                                                                                       <  1:19937/248‑1 (MQ=255)                                                                                                                                    aaGCATCACCTCCTGCGTCTTGCTCGCCAGCTCCTCCAGCACTTCGGCTCCGTTCTCAAACCGCTGACATGTTAAACCTGCCCCAGCCAGCGCCCGTTCTAACGCCCATCGTATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAAGCAGGTATACCGAGAACTCGGTATGCCCTTGCCAACTGGTAAATTTAATTTTGCCTGAATGCTGatcaat                                                                                                  <  2:275168/251‑1 (MQ=255)                                                                                                                                    aaGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTTGTAAATTCAATTTTGCCTGAATGCTGatcaat                                                                                                  <  1:315079/251‑1 (MQ=255)                                                                                                                                           acaTCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAAtt                                                                                            >  1:119121/1‑250 (MQ=255)                                                                                                                                               ccgccGTTTTGCTCGCCATAGGCTCCAGCACTGCTGCGCCGTTCTCAACCGTCGTACACGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCACCGGATGGAACGATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCTTGATAGGCAGGTAAACCGCGAACGTGGTATGCCCTGGCCAGCTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTCCGa                                                                                        <  2:272595/246‑1 (MQ=255)                                                                                                                                                cGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGc                                                                                      >  2:73983/1‑251 (MQ=255)                                                                                                                                                       ttGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGa                                                                                <  2:396349/250‑1 (MQ=255)                                                                                                                                                          cTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATa                                                                              >  2:94377/1‑249 (MQ=255)                                                                                                                                                                               tCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTcg                                                       >  1:81090/1‑251 (MQ=255)                                                                                                                                                                               tCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTc                                                        <  1:1788/250‑1 (MQ=255)                                                                                                                                                                                  gcgcCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTcgcg                                                     <  1:299823/250‑1 (MQ=255)                                                                                                                                                                                    gcCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGcc                                                  >  2:152581/1‑251 (MQ=255)                                                                                                                                                                                           tcAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCAGCTGAcc                                           >  1:45318/1‑251 (MQ=255)                                                                                                                                                                                               aCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTAGTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGAACATCg                                       <  2:113080/251‑1 (MQ=255)                                                                                                                                                                                                cgtcgtACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACAGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTGCGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCgg                                      <  1:12707/251‑1 (MQ=255)                                                                                                                                                                                                   cgtACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGt                                    <  2:144934/250‑1 (MQ=255)                                                                                                                                                                                                                   gCCCCAGCGAGCGCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCGTATCCTg                    <  1:172227/250‑1 (MQ=255)                                                                                                                                                                                                                       cggcgagcgCACGTTCAAAAACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAAATCGGTATGCCCTGGCCAACTGGTAAGTTCAATTTTGCCTGAATGCTGATAAATCAACATACGAGCGATGGATAAACCAAGCCCGGGGCCACATTCGCGGCCGATGACCAGCGGGAAACACAGCGTATCCTGCAAAt               >  2:384046/3‑251 (MQ=255)                                                                                                                                                                                                                         gcgagcgCACTTTCAAACTCCCAACGGATGGAACTGTCGTGATAGACTACCCAGGCTATCCCTAGTCGCATAAACGTCAACTTTATTTCCTGATAGGCAGGTAAGCAGAAAACTCGGAATGCGCTGGACAACTGGAAAATTAAATTATGCCTAACTGCTGATCAATCAAATTACGAGCGATGGATAAGCGAAGCCCGGGGCCACATGCGCGACCGCTGACCATGGGGTAAAACTGCggctcttgcaan                >  2:367446/1‑237 (MQ=255)                                                                                                                                                                                                                          cgagcgCACGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCGTATCCTGCAAATg              >  2:283089/1‑249 (MQ=255)                                                                                                                                                                                                                            agcgCACGTTCAAACACCCAACGGATGGAACTTTCGTCATCGACTACCCATACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGATAACTCTGTAGACCCCGGCCAACTGGTAAATTCAATTTGGCCTGAATGCTGATCAATCTAATTACGGGCGATGGATATGCCAAGCCCGGTGCCACCTTCGCGGGCGCTTCCCATCGCGTAAAGCAGCGGAAGCTGTAAATGAGGc          <  2:373313/251‑1 (MQ=255)                                                                                                                                                                                                                                  cGTTCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTAGTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACGGGTAAATTCAATTTTGCCTGAATGCTGATGAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGGGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCGTATCCTGCAAAGGAGGCGGAATg    <  2:248951/251‑1 (MQ=255)                                                                                                                                                                                                                                    ttCAAACACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCGTATCCTGCAAATGAGGCGGAATgcc  >  2:350348/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      TGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCGTATCCTGCAAATGAGGCGGAATGCC  >  NC_000913/4054992‑4055469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑