Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,016,990	G→A	W187* (TGG→TAG)	udp →	uridine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,016,990	0	G	A	100.0%	172.5 / NA	49	W187* (TGG→TAG)	udp	uridine phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/23);  total (26/23)

GATGTCCTGGTTACCACGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAT  >  NC_000913/4016746‑4017236                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       gATGTCCTGGTTACCACGGCGTCTGTCCGTCGGGATGGCGCGAGCCTGCACTTCGCAACGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAgg                                                                                                                                                                                                                                                   <  1:17930/250‑1 (MQ=255)      ccTGGTTACCACGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATgg                                                                                                                                                                                                                                             <  2:346544/251‑1 (MQ=255)             cccACGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTCTGGAAGAGTAGCAGGCGATGTGCGTAAt                                                                                                                                                                                                                                      <  2:43919/250‑1 (MQ=255)               cACGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATg                                                                                                                                                                                                                                     >  1:361481/1‑250 (MQ=255)                ccGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGaa                                                                                                                                                                                                                                   <  1:256777/250‑1 (MQ=255)                   gCGTCTGTCCGTCTGGATGGCGCTAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGGTGGAAGCTGCGAAATCCATTGGCGCGACAACGCACGTTGGCGTGACAGCTTCTGCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGAAGCTGGCGATGGGCGTAATGAACTa                                                                                                                                                                                                                                <  2:159667/251‑1 (MQ=255)                         gTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGa                                                                                                                                                                                                                             <  1:293344/248‑1 (MQ=255)                            cGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATgg                                                                                                                                                                                                                        >  1:251851/1‑250 (MQ=255)                                          gAGCCTCCACTTCGAACAGCTGGAATTCCCGGCTCTCGCTGATTTCGAATGTACGACTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACActgc                                                                                                                                                                                                         >  1:303260/1‑251 (MQ=255)                                             ccTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGGTTGAAGCTGCGAAGTCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGGTCTATGGAATAGTAGCAGGCGATGGTCGTAATGAACTATGAAATGGAATCTGCAACCctgctg                                                                                                                                                                                                       >  2:5795/1‑250 (MQ=255)                                               tGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGCCTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGAcc                                                                                                                                                                                                    >  2:58225/1‑251 (MQ=255)                                                       gCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGc                                                                                                                                                                                            <  1:107636/251‑1 (MQ=255)                                                            gCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCTTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTc                                                                                                                                                                                       <  1:162885/251‑1 (MQ=255)                                                             cTGGAATTCCCGGCAATCGCTGATTTCGAATGTACGACTGCGCTGGTTGAAGGTGCGAAATCCATTGGCGAGACAACTCAAGTTGGCGTGACAGCCTCTTCTGATACCTTCTACCCAGGTCAGGAAGGTTACGATACTTACTCTGGTCGCGTAGCTAGTGACTTTCAAGGTTCTACGGAAGTGTAGCAGGCGCTGGGAGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGagcacgagtgcacgtc                                                                                                                                                                                       >  2:35106/1‑235 (MQ=255)                                                                        ggCTGTCGCTGATCTCGAATGTACGACTGCGCTGGTTGAAGCTGAGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATAATTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACAATGAAATGGAACCTGCAACCCTGCTGCCAATGTGTGCAAGTCAGAGCCTGCGTg                                                                                                                                                                           >  2:401108/1‑251 (MQ=255)                                                                         gCTGTCGCTGATTTCGAATGTACGACTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGc                                                                                                                                                                          >  1:346116/1‑251 (MQ=255)                                                                                        gAATGTACGACTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTACTGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCggg                                                                                                                                                           >  2:30447/1‑251 (MQ=255)                                                                                            gTACGACTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGtt                                                                                                                                                       <  1:56849/251‑1 (MQ=255)                                                                                                            tGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCAccc                                                                                                                                       >  2:39075/1‑251 (MQ=255)                                                                                                                  tGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCa                                                                                                                                   >  1:388001/1‑249 (MQ=255)                                                                                                                     gAAATCCATTGTGGCGTCAACTCACGTTGGCGTGACAGCTTTTTCTGATACCTTCTACCCAGGTCAGGAACGATACGATACTTATTCTGGTCGCGTAGCTCGTCCCTTTCAAGGTTCTATGGAAGAGTAGCAGGCGATGCGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCCGACCATGTGGGCAAGACAGGGCCTGCTTGCCGGTCTGGTAGCGGGTGTGATCGGTAACGGAACCCAGCAag                                                                                                                                 >  2:8747/1‑248 (MQ=255)                                                                                                                               ggCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATg                                                                                                                     <  2:79411/250‑1 (MQ=255)                                                                                                                                          ccACGTTGGCGTGACAGCTTCTTATGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGa                                                                                                         <  2:309129/250‑1 (MQ=255)                                                                                                                                             cGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGaaac                                                                                                      >  1:20513/1‑251 (MQ=255)                                                                                                                                                   cGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCATGTATGGTAGCGGGTGTTATCGTTAAACGCACCCAGCAAGAGTTCCAGAATGCTGAGACGATGAAACAAACCg                                                                                                >  2:287269/1‑251 (MQ=255)                                                                                                                                                    gtaaCAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATTGAAGAGTAGCAGGCGATAGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGGTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGGCGATGAAACAAACCGa                                                                                               <  2:390377/248‑1 (MQ=255)                                                                                                                                                       aCAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACGCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCAGGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAg                                                                                            <  1:156627/251‑1 (MQ=255)                                                                                                                                                             tcttctGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCAt                                                                                        <  2:175448/249‑1 (MQ=255)                                                                                                                                                             tcttcgGATACGTTCTACCCAGGTCAGGAACGCTACGACACTTACTCTGGACGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGACGATGGGCGTAATGAACTATGAAATGGCATCTGCAACCCTGCTGCTCCTGTGTGCAACTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGTTCCCGAATGCTGTGACGTTGAAACAAACCGCAAGCCNTGc                                                                                      >  2:354565/1‑251 (MQ=255)                                                                                                                                                                 ctGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGCTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCACGGCCTGCGTGCCGGTATGGAAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCGATGCggcg                                                                                  >  1:57272/1‑249 (MQ=255)                                                                                                                                                                   gATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGa                                                                                 <  1:62767/250‑1 (MQ=255)                                                                                                                                                                       ccTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATc                                                                            <  2:340383/251‑1 (MQ=255)                                                                                                                                                                                cAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGAGCCCGAATGCTGATACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGaa                                                                   <  1:55909/251‑1 (MQ=255)                                                                                                                                                                                  ggTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCCACCCTGCTGACCATGTGTGCAAGTCGGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATg                                                                                                                     <  1:332682/199‑1 (MQ=255)                                                                                                                                                                                  ggTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAGCCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGGGAAAATCGTGGTTGAAgc                                                                 <  2:249222/251‑1 (MQ=255)                                                                                                                                                                                  ggTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATg                                                                                                                     >  2:332682/1‑199 (MQ=255)                                                                                                                                                                                     cAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAgcggc                                                              >  2:71921/1‑251 (MQ=255)                                                                                                                                                                                         aaCGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGcgt                                                          <  1:216907/251‑1 (MQ=255)                                                                                                                                                                                         aaCGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGcgt                                                          >  1:195241/1‑251 (MQ=255)                                                                                                                                                                                                      ttactCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGGGGAAGCGGCGCGTCGTCTGCTGTAAt                                             >  1:188333/1‑251 (MQ=255)                                                                                                                                                                                                                    tAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCTGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTc                               >  2:115830/1‑251 (MQ=255)                                                                                                                                                                                                                     aGTTCGTCACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCt                              >  1:337346/1‑251 (MQ=255)                                                                                                                                                                                                                     aGTTCGACACTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATAGGCGTAATGAAATATAAAATGGAATCTGCAACACTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGAAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCt                              >  1:249413/1‑251 (MQ=255)                                                                                                                                                                                                                             aCTTTAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCg                      <  1:145615/251‑1 (MQ=255)                                                                                                                                                                                                                               tttAAAGGTTCTATGGAAGAGTAGCAGGCGATGGGAGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGAGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTATTCTCCTGTCTGAAGGCCg                      >  2:156948/1‑249 (MQ=255)                                                                                                                                                                                                                                  aaaGGTTCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGAcgcg                 <  1:39075/251‑1 (MQ=255)                                                                                                                                                                                                                                       ttCTATGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCTAGAGATCCCGAATGCTGAGACGATTAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCgg            >  1:286540/1‑251 (MQ=255)                                                                                                                                                                                                                                           aTGGAAGAGTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCt         <  2:343692/250‑1 (MQ=255)                                                                                                                                                                                                                                                 gagTAGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCCTGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAt  >  1:140078/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       GATGTCCTGGTTACCACGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCAACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAT  >  NC_000913/4016746‑4017236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑