Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,913,050	G→A	L207F (CTT→TTT)	glmS ←	L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,913,050	0	G	A	98.4%	216.1 / ‑4.2	62	L207F (CTT→TTT)	glmS	L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (38/23);  total (39/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

GTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTT  >  NC_000913/3912808‑3913292                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   gtcgGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATa                                                                                                                                                                                                                                             >  1:151988/1‑250 (MQ=255)                  gCTCGTTTAAATCAACCTGACCGTGGCTGATGCGCCCGCTCAGGGTGTTTTTGATCGCGTTCGGCGGTTCGTAGATCGCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCCATATCCTGACGTTTTACTTCCGCGCCGGTTTTATCGACGAGGTTTACCGACCGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGAAGGGTCACCGGc                                                                                                                                                                                                                           >  2:348316/1‑251 (MQ=255)                      gCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAAc                                                                                                                                                                                                                        >  2:300801/1‑250 (MQ=255)                         tAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTAGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAg                                                                                                                                                                                                                      <  1:86688/249‑1 (MQ=255)                             tCAACCTGACCGTGGCTGATGCGTCAGGCAAGGGCGATTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTACGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGcc                                                                                                                                                                                                                  >  2:175298/1‑249 (MQ=255)                              cAACCTGACCGTGGCTGATGCGTCCGGCAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGc                                                                                                                                                                                                               >  1:103042/1‑251 (MQ=255)                                aCCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAAAAGCGCCAGCTg                                                                                                                                                                                                             >  2:172431/1‑251 (MQ=255)                                 ccttaCCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGATCGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTgg                                                                                                                                                                                                            <  2:352308/247‑1 (MQ=255)                                    gACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGGAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCa                                                                                                                                                                                                         <  2:402203/251‑1 (MQ=255)                                       cGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGa                                                                                                                                                                                                       >  2:200423/1‑250 (MQ=255)                                               aTGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGACAGATGGTCAGAAGCGATaa                                                                                                                                                                                               >  1:212762/1‑250 (MQ=255)                                                 gCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGt                                                                                                                                                                                            <  1:156495/251‑1 (MQ=255)                                                     ccGGTCAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTNTTc                                                                                                                                                                                        >  2:358978/1‑251 (MQ=255)                                                     ccGGTACGGGTGTGTTTGATCGCGTGCGGCTGTTCGTAGATCGCTTTTTGCATGTAGTGACGGTAAATGCCTTTATCGCCAGCATCATATTGCAGATTGGATTCGAGATCCTGACGTTTTGCTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAAGATCGCGCTCTTCAAAGAAGATAAAGAGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCAATAAAGTTTTc                                                                                                                                                                                        <  2:350737/251‑1 (MQ=255)                                                           aGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCAt                                                                                                                                                                                  <  2:337683/251‑1 (MQ=255)                                                           aGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGAAGGGTCACCGGCAACAGCGCCAGCTGGACAGAAGCGATAAAGTTTTCGCCCAt                                                                                                                                                                                  >  1:161857/1‑251 (MQ=255)                                                               tgtTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGACTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGCAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATCAAGTTTTCGCCCATcccc                                                                                                                                                                              >  2:289003/1‑251 (MQ=255)                                                                            gTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCAcc                                                                                                                                                                  >  1:227752/1‑250 (MQ=255)                                                                               cGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGc                                                                                                                                                               <  2:13012/250‑1 (MQ=255)                                                                                    gTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGGTTTCGCCCATCCCCAGGCCAATCACCAGCGGACt                                                                                                                                                          <  1:346854/250‑1 (MQ=255)                                                                                           gATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAAc                                                                                                                                                  >  2:156739/1‑251 (MQ=255)                                                                                                ctTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGCCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTgcc                                                                                                                                             >  1:381292/1‑251 (MQ=255)                                                                                                      gCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAATAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGaa                                                                                                                                                   <  2:174784/239‑1 (MQ=255)                                                                                                      gCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGaa                                                                                                                                                   >  1:174784/1‑239 (MQ=255)                                                                                                            aGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTa                                                                                                                                 <  2:151988/251‑1 (MQ=255)                                                                                                               gACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATcc                                                                                                                              >  2:167558/1‑251 (MQ=255)                                                                                                                aCGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCg                                                                                                                             <  1:156739/251‑1 (MQ=255)                                                                                                                    tAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTg                                                                                                                         >  1:22821/1‑251 (MQ=255)                                                                                                                    tAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGCATCCGGGTg                                                                                                                         >  2:114162/1‑251 (MQ=255)                                                                                                                      aaTGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGCATCCGGGTGAc                                                                                                                       >  1:389658/1‑251 (MQ=255)                                                                                                                              tATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCgg                                                                                                                                                                                                                            <  2:137859/142‑1 (MQ=255)                                                                                                                              tATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCgg                                                                                                                                                                                                                            >  1:137859/1‑142 (MQ=255)                                                                                                                                        gTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTg                                                                                                     >  2:99072/1‑251 (MQ=255)                                                                                                                                         tCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGCTTTTCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGTGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGAAGGGCATCCGGGTGACGGGAGACCATGATCACTGt                                                                                                    >  2:184545/1‑251 (MQ=255)                                                                                                                                              ttGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGt                                                                                               >  1:13801/1‑251 (MQ=255)                                                                                                                                                 cAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACg                                                                                            <  1:167558/251‑1 (MQ=255)                                                                                                                                                          ttCGATATCCTGACGTTTTACTTCAGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACt                                                                                                                                                          >  2:90128/1‑180 (MQ=255)                                                                                                                                                          ttCGATATCCTGACGTTTTACTTCAGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACt                                                                                                                                                          <  1:90128/180‑1 (MQ=255)                                                                                                                                                            cGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAg                                                                                  <  2:56481/250‑1 (MQ=255)                                                                                                                                                              atatCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTg                                                                               <  1:114162/251‑1 (MQ=255)                                                                                                                                                                atCCTGACGTCTTCCTTCAGCGCAAGATTTATCGAAGATGTTTCCCGAACGACACGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGCTCAGAAGCGCTAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGTACTACCAGAACATGCCGCCAGCAGGGTAACCGGGTGACGGCAGACCAAGATCACTGTACCCTACGCACCACGCCGCTGCg                                                                             >  2:56171/1‑251 (MQ=255)                                                                                                                                                                   cTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCCCGCAGCTGCggg                                                                           >  2:294531/1‑250 (MQ=255)                                                                                                                                                                     gACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCTCCACGCAGCTGCGGGATa                                                                        <  1:300801/251‑1 (MQ=255)                                                                                                                                                                     gACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGAt                                                                         >  2:155133/1‑250 (MQ=255)                                                                                                                                                                      aCGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAG‑‑GGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGATAGc                                                                      >  1:47855/1‑250 (MQ=255)                                                                                                                                                                        gTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGACGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAACTGGTCAGAAGCGATAAAGTTTTCGCCCATCCACAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGAATCCGGGTGACGGGAGTCCATGATCATTGTACCGTACGCACCACGCCGCTGCGGGATAGcc                                                                     >  2:390892/1‑250 (MQ=255)                                                                                                                                                                         ttttACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCGTCAAAGAAGATAAAGCGACGGGTCACCGTCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCa                                                                     <  2:254900/250‑1 (MQ=255)                                                                                                                                                                           ttACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGCTAGCACGc                                                                  >  2:120037/1‑251 (MQ=255)                                                                                                                                                                               ttCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGa                                                               >  2:207611/1‑250 (MQ=255)                                                                                                                                                                                  cgcgCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACgg                                                           >  1:125929/1‑251 (MQ=255)                                                                                                                                                                                      ccAGTTTTATCGAAGCTGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTTAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGATGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTc                                                       >  1:231938/1‑251 (MQ=255)                                                                                                                                                                                                 gAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTcc                                            <  2:284683/251‑1 (MQ=255)                                                                                                                                                                                                 gAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTcc                                            >  1:285117/1‑251 (MQ=255)                                                                                                                                                                                                    gATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGACAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTACCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTAACGCAGAGTCCCGc                                         >  2:123918/1‑251 (MQ=255)                                                                                                                                                                                                             cGAACGGCGAGTGATGTCCGCAATATCGCCCTCGTCAAAGAAGATAAAGCGACGGGTGACCGGCAACAGCGCCAGCTGGTCAGAAGCGACAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAGCGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTGCCGTACTTACGACGCAGCCGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCa                                <  1:219023/251‑1 (MQ=255)                                                                                                                                                                                                               aaCGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCcagc                                                                                                                                       <  2:336001/146‑1 (MQ=255)                                                                                                                                                                                                               aaCGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCcagc                                                                                                                                       >  1:336001/1‑146 (MQ=255)                                                                                                                                                                                                                          aTTTCCGCAATATCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCAc                   <  1:38903/251‑1 (MQ=255)                                                                                                                                                                                                                                    tatCGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGc          >  2:283045/1‑250 (MQ=255)                                                                                                                                                                                                                                       cGCCCTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGACCGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATc      >  2:264012/1‑251 (MQ=255)                                                                                                                                                                                                                                         cccTCTTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCCGGCCAACCACCAGCGGACTCCCCGAACGTTCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCAc    <  2:45638/251‑1 (MQ=255)                                                                                                                                                                                                                                           ctctTCAAAGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACtt  >  2:304136/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   GTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTT  >  NC_000913/3912808‑3913292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑