Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,884,712	C→T	R74C (CGT→TGT)	rnpA →	RNase P protein component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,884,712	0	C	T	100.0%	121.7 / NA	34	R74C (CGT→TGT)	rnpA	RNase P protein component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/17);  total (17/17)

CTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCGCCTGGCTCGCGGGTCCTGATAGCCCTCATTCGGGTCTATCAACGCCTGATTAGTCCGCTACTCGGGCCGCATTGTCGTTTCACTCCAACCTGTTCAAGCTACGGAATTGAGGCATTGCGCAGG  >  NC_000913/3884469‑3884956                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     cTAAGTAATATGGCTAAACCCTTAGCGCTTAAGCAAGCATTTCCCCGGGCGTTACGCTTGTTAACTCCCAGTCAATTAACATTCGTCTTACAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGACGCCTGGATTCGCTGGGGCATCCCCGTTTCGTTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCACTCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTgcg                                                                                                                                                                                                                                               >  2:250364/1‑251 (MQ=255)          aaaGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATg                                                                                                                                                                                                                                       <  2:213270/250‑1 (MQ=255)            aGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTACCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAAAATGAAc                                                                                                                                                                                                                                    >  1:34045/1‑251 (MQ=255)                         gTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATgg                                                                                                                                                                                                                        >  1:219010/1‑250 (MQ=255)                                              aGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGaaaa                                                                                                                                                                                                   >  2:324329/1‑250 (MQ=255)                                                     tACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGtt                                                                                                                                                                                           >  1:42250/1‑251 (MQ=255)                                                          ttGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTATGCGCCAACATGAACTCCCGGCTATGGATTTCGCGGTGGTGGCGCAAAAAGGGGTTGCCg                                                                                                                                                                                       >  2:241678/1‑250 (MQ=255)                                                           tGTTAACTCCCAGTCAATTCACATTGGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCg                                                                                                                                                                                       <  1:159152/249‑1 (MQ=255)                                                                     cAGTAAATTCACATTCGTCTTCCAGCAGCCACAACGAGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAAcc                                                                                                                                                                           <  2:221566/251‑1 (MQ=255)                                                                       gTCAATTCCCATTCGTCTTCCAGCAGCCACAACTGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGt                                                                                                                                                                         <  1:70486/251‑1 (MQ=255)                                                                                      tCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCg                                                                                                                                                          >  2:208867/1‑251 (MQ=255)                                                                                        ttCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGtt                                                                                                                                                        >  2:108433/1‑251 (MQ=255)                                                                                            agcagcCACATCGGGCGGGCACGCCGCAAATTACCATTCTCGTCCGCCTGAATTCGCTTGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACTTCTTACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCTTGGTGGTGGGGAAAAAATGTGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGaa                                                                                                                                                    <  1:170239/251‑1 (MQ=255)                                                                                              cagcCACAACGGGCTGGCACGACGCAAATTACCATTCTCTGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGACAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGTAAAAAGGGGTTGCCGACCTCGATAACCGTGCGCTCTCGGTAGCGGTGGaaa                                                                                                                                                   >  2:200103/1‑250 (MQ=255)                                                                                                     aaCGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGa                                                                                                                                                                                                                       <  1:340455/175‑1 (MQ=255)                                                                                                     aaCGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGa                                                                                                                                                                                                                       >  2:340455/1‑175 (MQ=255)                                                                                                        gggCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGgcgc                                                                                                                                        <  1:108433/251‑1 (MQ=255)                                                                                                                                    cggtcgcatGAATTCGCTGGTGCATCCCCGTACCGGGATAACAGTAACCAAAAAAATCGTTCGTCGCGCCAATTAGCTCAACCGGAGTAATCTTGGGAGACGTGATAGCTTCTGTCGTCGCCAACAGTAGCTCCCGGCTATGGATTCCGTGGTGGTGGAGAAAAAAGTGGTTGCTGCCCCCGATAACCGTGCCCACCCGGAGGCGGTGGAAAATTTATTGTGCCGCCACTGTCGCCTGGCTCGCGGGTcc                                                                                                             <  2:334934/242‑1 (MQ=255)                                                                                                                                    cGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCGCCTGGCTCGCGGGTc                                                                                                              <  1:324329/249‑1 (MQ=255)                                                                                                                                                   gCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAAAGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGACAAATTATGGCGCCGCCACTGTCGCCTGGCTCGCGGGTCCTGATAGCCCTCATTCg                                                                                             >  1:165983/1‑251 (MQ=255)                                                                                                                                                          cATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACGGTCGCCTGGCTCGCGGGTCCTGATAGCCCTCATTCGGGTCTAt                                                                                      <  2:51197/251‑1 (MQ=255)                                                                                                                                                                        tCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAAAGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGACAAATTATGGCGCCGCCACTGTCGCCTGGCTCGCGGGTCCTGATAGCCCTCATTCGGGTCTATCAACGCCTGAtt                                                                          >  1:180551/1‑249 (MQ=255)                                                                                                                                                                           tACAGGCGCAAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCGCCTGGCTCGCGGGTCCTGATAGCCCTCATTCGGGTCTATCAACGCCTGATTAGt                                                                       <  1:322124/249‑1 (MQ=255)                                                                                                                                                                                   ccAAGAAAAACGTGCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGGGAAAGCTTCTGTCTGCGCCACCATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGCCCTCGATAACCGTGCTCTCTCGGAAGCCTTGGAAAAATTATGGCGCCGCCACTGTCGCCTGGCTCGCGGGTCCTGATAGCCCTCATTCGGGTCTATCAACGCCTGATTAGTCCGCTACTCg                                                             <  2:42250/251‑1 (MQ=255)                                                                                                                                                                                     aaGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCGCCTGGCTCGCGGGTCCTGATAGCCCTCATTCGGGTCTATCAACGCCTGATTAGTCCGCTACTCggg                                                           >  1:361936/1‑251 (MQ=255)                                                                                                                                                                                      aGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCGCCTGGCTCGCGGGTCCTGATAGCCCTCATTCGGGTCTATCAACGCCTGATTAGTCCGCTACTCGGGc                                                          >  2:353294/1‑251 (MQ=255)                                                                                                                                                                                              gTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCGCCTGGCTCGCGGGTCCTGATAGCCCTCATTCGGGTCTATCAACGCCTGATTAGTCCGCTACTCGGGCCGCATTGt                                                  >  2:79389/1‑251 (MQ=255)                                                                                                                                                                                                gcNACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCGCCTGGCTCGCGGGTCCTGATAGCCCTCATTCGGGTCTATCAACGCCTGATTAGTCCGCTACTCGGGCCGCATTGTc                                                 <  2:361936/249‑1 (MQ=255)                                                                                                                                                                                                              aaCGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCGCCTGGCTCGCGGGTCCTGATAGCCCTCATTCGGGTCTATCAACGCCTGATTAGTCCg                                                                    >  2:278495/1‑217 (MQ=255)                                                                                                                                                                                                              aaCGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCGCCTGGCTCGCGGGTCCTGATAGCCCTCATTCGGGTCTATCAACGCCTGATTAGTCCg                                                                    <  1:278495/217‑1 (MQ=255)                                                                                                                                                                                                                         aTTAACCGTCTGAGGCGTGAAGGCTTCTGTCTGCGCCAACAGGAACTCCCGGCTATGGATTTCGTGGTGGGGGAGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGATGCGTTGAAAAAATTATGGCGCCGCCACTGTCGCCTGGCTCGCGGGTCCTGATAGCCCTCATTCGGGTCTATCAACGCCTGATTAGTCCGCTACTCGGGCCGCATTGTCGTTTCACTCCAACCTGTTCAAGCTAc                       <  2:8534/251‑1 (MQ=255)                                                                                                                                                                                                                                      cgcgTGAAAGCTTCTGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTTTCTCGGAAGCGTGGGAAAATTTAGGGAGCCGCAACTGTCGCCTGCCTCGCGGGTCCTGATAGCCCTCATTCGGTCCTTTCAACGCTTGTTTATTCCGCTACTGGGCCCGCATTGTGGTTTCACTCCAACCTGTTCAACCTACGGAATTGAGGctt          >  1:245084/1‑249 (MQ=255)                                                                                                                                                                                                                                        cgTGAAAGCTTCTGTCTGCGCCAACATGCACTCCCGGCTATGTATTTCGTGGTGGTGGCGAAAAAAGGGTTTGCCGCCCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCGCCTGGCTCGCGGGTCCTGATAGCCCTCATTCGGGTCTATCAACGCCTGATTAGTCCGCTACTCGGGCCGCATTGTCGTTTCACTCCAACCTGTTCAAGCTACGGAATTGAGGCATTg        <  1:61513/251‑1 (MQ=255)                                                                                                                                                                                                                                              aGCTTCTGTCTGCGCCAACATGAACCCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGCCCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCGCCTGGCTCGCGGGTCCTGATAGCCCTCATTCGGGTCTATCAACGCCTGATTAGTCCGCTACTCGGGCCGCATTGTCGTTTCACTCCAACCTGTTCAAGCTACGGAATTGAGGCATTGCGCAgg  <  2:131698/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     CTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCGCCTGGCTCGCGGGTCCTGATAGCCCTCATTCGGGTCTATCAACGCCTGATTAGTCCGCTACTCGGGCCGCATTGTCGTTTCACTCCAACCTGTTCAAGCTACGGAATTGAGGCATTGCGCAGG  >  NC_000913/3884469‑3884956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑