Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,838,773	C→T	P176S (CCC→TCC)	yicL →	putative transporter YicL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,838,773	0	C	T	100.0%	104.4 / NA	29	P176S (CCC→TCC)	yicL	putative transporter YicL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/11);  total (18/11)

CATCGAAAAATCGAACGCAGCCACGGCAACGGTGCTGCAATTCCTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATT‑GGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACC  >  NC_000913/3838526‑3839014                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   cATCGAAAAATCGAACGCAGCCACGGCAACGGTGCTGCAATTCCTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCt                                                                                                                                                                                                                                                 >  2:174835/1‑251 (MQ=255)         aaTCGAACGCAGCCACGGCAACGGTGCTGCAATTCCTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCt                                                                                                                                                                                                                                          >  2:35637/1‑250 (MQ=255)            cGAACGCAGCCACGGCAACGGTGCTGCAATTCCTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAAtt                                                                                                                                                                                                                                      <  2:101638/251‑1 (MQ=255)                     ccACGGCAACGGTGCTGCAATTCCTCTCACCGACGATTATCGCCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACCCGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTAt                                                                                                                                                                                                                             >  2:286421/1‑251 (MQ=255)                       aCGGCAACGGTGCTGCAATTCCTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATgg                                                                                                                                                                                                                           >  1:366548/1‑251 (MQ=255)                        cGGCAACGGTGCTGCAATTCCTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATGGc                                                                                                                                                                                                                          >  1:57312/1‑251 (MQ=255)                                                    gacgaTTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGc                                                                                                                                                                                              <  1:127094/251‑1 (MQ=255)                                                    gacgaTTATCGTCGCCTGGTTATCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGc                                                                                                                                                                                              >  1:85031/1‑251 (MQ=255)                                                           aTCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGCTTgg                                                                                                                                                                                       >  2:68153/1‑251 (MQ=255)                                                           aTCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCATCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGAGTgg                                                                                                                                                                                       >  2:139316/1‑251 (MQ=255)                                                             cgtcgCCTCGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTCTTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGTGCATTGCTTCGGCATTTGCTGCTTCATTCTATACCACCTATTCCTCAACGCTAATGGCCCTCTATGGCCCGTTACCCGTCGTTTTCTGGAGTATGCTTATTgtcg                                                                                                                                                                                     <  2:67853/251‑4 (MQ=255)                                                               tcgCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTTTGTTTTTCTGAGCTATTTTGACATCGCTGGTCTGGACTTTTTTATTGGTGACACACGGTAACCCGACGTCATTATCGATCTCTCCTGCTGCGTTGTTCTGGGGCATTGCCTCGGCAGTTGCTGCTGCATTCTATACCACCTAGTCCTCAACGCTAAGTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTgtcg                                                                                                                                                                                     <  2:116798/249‑4 (MQ=255)                                                                                       aaTCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGcc                                                                                                                                                           >  1:168205/1‑251 (MQ=255)                                                                                                                            gACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCATCGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGGCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTGACGGCAGTTTg                                                                                                                       <  1:393400/250‑1 (MQ=255)                                                                                                                             aCATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGAt                                                                                                                     >  1:10203/1‑251 (MQ=255)                                                                                                                                        cGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGttt                                                                                                           <  1:200409/250‑1 (MQ=255)                                                                                                                                         gggACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGttttt                                                                                                         <  1:131167/251‑1 (MQ=255)                                                                                                                                                    aTTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTTCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGAATCTGGCGTTTTTTTATTtggtgg                                                                                              >  1:305113/1‑251 (MQ=255)                                                                                                                                                     ttGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGTGTTTTTTTATTTgggggt                                                                                             >  1:67149/1‑251 (MQ=255)                                                                                                                                                                cGGTACTCCGACGTCATTATCGATCTCTCCTGCTGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTCCCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAAGAACAAACTTTGTCGTTAACGGCAGTTTGATTTTGGGGTTTTTTTTTTTGGCGGTCATT‑TGTActt                                                                                 >  2:390284/1‑249 (MQ=255)                                                                                                                                                                      tCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGCTTCTGTTGCCTTTTTATGCCAGACaa                                                                                                                                                     >  1:65772/1‑178 (MQ=255)                                                                                                                                                                      tCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACaa                                                                                                                                                     <  2:65772/178‑1 (MQ=255)                                                                                                                                                                                        ctctcCTGCCGCGTTGTTCTGGGGCATGGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGACCGCTATGGCACGTTACCAGTCGTCGGCTGGCGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGGGGTTTTTTTTTTGGTGGTCATT‑GGTATGGACCTGACATTTTGTCTGTAccat                                                          >  2:330440/1‑248 (MQ=255)                                                                                                                                                                                                   cgTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATT‑GGTACGTCCCTGACATTTTGTCTGTACCTGAAAGGAGcaca                                               >  1:328623/1‑250 (MQ=255)                                                                                                                                                                                                     ttgttCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCCGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTTATTCTGTAGTTTTTTTATTTTGGGGTCATTTGGT‑CGTCCCTTACTTTTTGTCTTTACCTGTAAGGAgaaaaatt                                            >  2:79892/1‑244 (MQ=255)                                                                                                                                                                                                                               tgctgctgcATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATT‑GGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCAtt                   <  1:288805/250‑1 (MQ=255)                                                                                                                                                                                                                                      gcATTCTATACCACCTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCATACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATT‑GGTACGTCCCTGACATTTAGTCTGTACCTGAAATTAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCtg           <  2:57312/251‑1 (MQ=255)                                                                                                                                                                                                                                              taCCACCTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATT‑GGTACGTCCCTGACAttt                                                                      <  1:152538/184‑1 (MQ=255)                                                                                                                                                                                                                                               accaccTATTCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGCTTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGAGTTTTTTTATTTGGGGGTCATT‑GGTACGTCCCTGACCTTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCACCCTTTTGTGGTGTGcctaaac  >  2:244448/1‑245 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   CATCGAAAAATCGAACGCAGCCACGGCAACGGTGCTGCAATTCCTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATT‑GGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACC  >  NC_000913/3838526‑3839014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑