Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,494,388	Δ1 bp	coding (684/924 nt)	ydcI ←	DNA‑binding transcriptional dual regulator YdcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,494,388	0	C	.	97.7%	192.8 / ‑4.1	43	coding (684/924 nt)	ydcI	DNA‑binding transcriptional dual regulator YdcI
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base . (17/25);  total (18/25)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGG  >  NC_000913/1494143‑1494629                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  aCCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAgg                                                                                                                                                                                                                                              >  1:248265/1‑250 (MQ=255)  ccTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGaa                                                                                                                                                                                                                                            <  1:291397/251‑1 (MQ=255)     ttCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAAtt                                                                                                                                                                                                                                          <  2:375744/250‑1 (MQ=255)       cAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAAtttt                                                                                                                                                                                                                                        <  2:43548/250‑1 (MQ=255)         gAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTAGCATCTACGCGGGTCAGTATTCCACTCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCCGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTAc                                                                                                                                                                                                                                      >  1:221913/1‑250 (MQ=255)                    aTTTTCGAATAGCGTTAATCATCAACTGGCAACCGGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGATATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTTCAGCCCTGGctct                                                                                                                                                                                                                          <  2:174879/251‑1 (MQ=255)                       ttCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGc                                                                                                                                                                                                                        <  2:106570/250‑1 (MQ=255)                                     atcatcAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGACGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGACTTTTACAGCCCTGGCCCGGCACTAATGCATCTGa                                                                                                                                                                                                          >  2:236269/1‑250 (MQ=255)                                     atcatcAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGAGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGAAAGCGTTTCGATACAACC‑GAAGGACTTTTACAGCCCTGGCTCCGCACTAATGCATCTGa                                                                                                                                                                                                          >  2:163548/1‑250 (MQ=255)                                       catcaACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAAt                                                                                                                                                                                                        <  1:146454/250‑1 (MQ=255)                                                 cAACCAGAATAGAACGTCTCATCTACGCGGGGCAGTATTCCAATCGGTTCGCCTGCGCCATGTCCCGGATCAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTTAGCGTATTCAACCGTAAGTTGACGAGATAGCGAATCAGACAGCGTTTCGATGCAACC‑GAAGGAATTTTACAGCCCGGGCTCTGCCCTAATTCATCTTAATGCttgcgtggc                                                                                                                                                                                             <  2:221758/251‑1 (MQ=255)                                                 cAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTACGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCtggcgtggc                                                                                                                                                                                             >  1:92529/1‑251 (MQ=255)                                                      agaagaGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGt                                                                                                                                                                                        >  2:3915/1‑251 (MQ=255)                                                                gTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGt                                                                                                                                                                              >  1:320596/1‑251 (MQ=255)                                                                  cGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTg                                                                                                                                                                             >  2:663/1‑250 (MQ=255)                                                                    cATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGAt                                                                                                                                                                           >  1:297778/1‑250 (MQ=255)                                                                            cgGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGGGATACAACGAcc                                                                                                                                                                  >  2:63693/1‑251 (MQ=255)                                                                                 cAGTATTCCAATCGTTTCGCCTGCACCATGTTCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTGTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAAGTTTACAGCCCTGGCTCTGCACTAATGCATCTGAAGGCTGGCGTGGAACAGGGCCTTCTGGTGATACAACGCCCGGCCa                                                                                                                                                             <  2:54106/251‑1 (MQ=255)                                                                                 cAGTATTCCAATCGGTTCGCCTGCCCCATGTCCCGGAACAGGCAGGGCCAACAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGAAAAAACACCAGTAATCGGATTAAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCa                                                                                                                                                             <  1:284515/251‑1 (MQ=255)                                                                                 cAGTATTCCAATCGGTTCGCCTGCACCATTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACGAATTCAGCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATGTAACGACCGGcc                                                                                                                                                              <  2:390087/250‑1 (MQ=255)                                                                                     aTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGGCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTcc                                                                                                                                                         <  2:302488/251‑1 (MQ=255)                                                                                                      tgtaccgtGTCCCGGAACGGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTCTTACAGTGCCAGAAGGGACAAACCACCCGTAATCGTATTCAACCGTCAGTTGACGAGATAGCGAAACATACATCGTTTCGATA‑AAACCGAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATGTGAATGCTGGCATGGCGCAGAACCTTCTGGGGATTCAAAGACCGGGCCTTTCAGCACCCGGCCTAgtgg                                                                                                                                        >  2:64973/8‑248 (MQ=255)                                                                                                         aCCATGTCCCGGACCAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTAc                                                                                                                                     >  2:77813/1‑251 (MQ=255)                                                                                                                  cGGACCAGGCACGGCCACCAGCCGGGCATGACGCAGTTCGTCTTATACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAATC‑GAAGGAATTTTACAGCCCTGGCTCTGCACGAATGCACCTGAATGCTGGCGTGGCGCAGTGCCTTCTTGTGATACAACTACGTGCCCTTCCAGCTGCCGGCTTAGCGTTACGtcatcc                                                                                                                              <  2:38352/249‑6 (MQ=255)                                                                                                                    gAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGaa                                                                                                                          <  2:254946/251‑1 (MQ=255)                                                                                                                      aCAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGTACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGTTGATACAACGACCGGCCATTCCAGCACCCGTCTTAGCGTTACGTTCTCCTGAAGt                                                                                                                        <  2:43198/251‑1 (MQ=255)                                                                                                                              ggCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCGCCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTg                                                                                                                <  2:248265/251‑1 (MQ=255)                                                                                                                                  accaGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTg                                                                                                                                                                                >  1:259395/1‑183 (MQ=255)                                                                                                                                  accaGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTg                                                                                                                                                                                <  2:259395/183‑1 (MQ=255)                                                                                                                                   ccaGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGAtt                                                                                                             >  1:115064/1‑249 (MQ=255)                                                                                                                                      gCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGc                                                                                                        <  2:35819/251‑1 (MQ=255)                                                                                                                                                     ggccgtcTTTTACAGCGCCAGAATGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCGGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCGTCCGGCTTAGCGTTACTTTCTCCTGAAGTAGCGGGTGATTAGGGCGTACAACCAGCTTCa                                                                                         <  1:228124/248‑1 (MQ=255)                                                                                                                                                             tttACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTc                                                                                  <  2:297778/250‑1 (MQ=255)                                                                                                                                                                        aGAAGGGACAAACCACACGTCATCGTATTCAACCGTACGTTGCCGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGTCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAAcagcat                                                                      >  1:124465/1‑250 (MQ=255)                                                                                                                                                                           aGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTc                                                                   >  2:107432/1‑251 (MQ=255)                                                                                                                                                                                        acGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCg                                                      <  1:223387/251‑1 (MQ=255)                                                                                                                                                                                            aaTCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGtca                                                  <  2:273945/251‑1 (MQ=255)                                                                                                                                                                                             aTCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGtcat                                                 <  1:185875/251‑1 (MQ=255)                                                                                                                                                                                                                 gACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCTGGTGATTAGGGCGTACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGAGCTGACATc                              <  2:221913/250‑1 (MQ=255)                                                                                                                                                                                                                           cGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGcc                   <  1:6068/251‑1 (MQ=255)                                                                                                                                                                                                                                           tCGATACATCC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATGAAGCCCGTTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTcccc    <  1:356959/250‑1 (MQ=255)                                                                                                                                                                                                                                           tCGATACAACC‑GAAGGAATTTTACAGCCCTGGATCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGAGCGGCCATTCCAGCATCCGGTTTAGCGTTACGTTCTCCTGAAGTAGCGGTAGATTAGGCGGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCGGGTCATCAGTTCACGAGCTGATCTCCGACCAATGACGatatggctttctccg   >  2:12017/1‑240 (MQ=255)                                                                                                                                                                                                                                            cGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCgg  >  1:265621/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  ACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGG  >  NC_000913/1494143‑1494629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑