Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	1,303,073	Δ12 bp	coding (175‑186/921 nt)	oppB →	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1303072	1 (0.020)	29 (0.720)	25/488	0.6	96.7%	coding (174/921 nt)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
	?	NC_000913	1303085 =	1 (0.020)					coding (187/921 nt)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

CTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTT  >  NC_000913/1302853‑1303330                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      gctccacggtgtctgatttttatcgcattacagaaggcacaggccagaaggtagggcaatgttaaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAaac                                                                                                                                                                                                                                     <  1:120847‑M2/19‑1 (MQ=255)                                 gaaggcacaggccagaaggtagggcaatgttaaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTTCCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGttt                                                                                                                                                                                                     <  1:334223‑M2/51‑1 (MQ=255)                                    ggcacaggccagaaggtagggcaatgttaaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTaa                                                                                                                                                                                                   >  2:99008‑M2/198‑250 (MQ=255)                                     gcacaggccagaaggtagggcaatgttaaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAat                                                                                                                                                                                                 <  1:82921‑M2/55‑1 (MQ=255)                                         aggccagaaggtagggcaatgttaaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATaa                                                                                                                                                                                             <  2:12916‑M2/59‑1 (MQ=255)                                          ggccagaaggtagggcaatgttaaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATaaa                                                                                                                                                                                            <  1:362660‑M2/60‑1 (MQ=255)                                                 aggtagggcaatgttaaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTAt                                                                                                                                                                                                                                                     >  2:8560‑M2/185‑187 (MQ=255)                                                 aggtagggcaatgttaaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTAt                                                                                                                                                                                                                                                     <  1:8560‑M2/3‑1 (MQ=255)                                                          aatgttaaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATg                                                                                                                                                                            >  1:84993‑M2/176‑251 (MQ=255)                                                                    tttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagaccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgcaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGc                                                                                                                                                                  >  1:31531‑M2/166‑251 (MQ=255)                                                                                 gctgtctggaagcgattccgacgctatttattcttattactatttcgttctttattatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTccc                                                                                                                                                     <  2:223250‑M2/99‑1 (MQ=255)                                                                                    gtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGtt                                                                                                                                                  >  1:217665‑M2/150‑251 (MQ=255)                                                                                         gaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGc                                                                                                                                             >  2:364985‑M2/145‑251 (MQ=255)                                                                                             cgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCaa                                                                                                                                          >  1:300727‑M2/141‑250 (MQ=255)                                                                                                ttacgacgctatttattcttattcctatttcgttctttatgatgcgcctcgcgccgggaatcccttttaccggcgaacgtactttaccgccagaagggatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGCGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAACCTg                                                                                                                                      >  2:18375‑M2/138‑251 (MQ=255)                                                                                                   cgacgctatttcttcttataaccatttcgttctttatgatccgcctcgcgccgggaagcccttttaccggcgaacgtactctaccgccagaagtgatggccaatatcgaagcgaactatcatcttaatgatccgTCTTTCAGCTACCTGAAACAACTGCCGCACGGTGTTTTCGGTCCATCGTTTAACTATACAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTTTGCCAAACTggg                                                                                                                                    >  2:42387‑M2/135‑250 (MQ=255)                                                                                                    gacgctatttattcttattactatttcgctctttatgatgcgcctcgcgccgggaatcccttttaccggcgaacgtactttaccgccagacgtgatggccaatatcgaagcgaaatatcatcttaatgatccgGATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAGAATTATTCGGTCAATGACTTGGTTGAATCCAGTTTTACCGTTTCTGCCAAACTggg                                                                                                                                    >  2:105292‑M2/134‑249 (MQ=255)                                                                                                               ttcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCAttt                                                                                                                       >  2:310964‑M2/123‑251 (MQ=255)                                                                                                                    attactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTcc                                                                                                                   <  2:300727‑M2/133‑1 (MQ=255)                                                                                                                             tcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTaa                                                                                                          <  1:99008‑M2/142‑1 (MQ=255)                                                                                                                                        gcCGGTAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAATTGAGGGCCAATATCGAAGCGAAATATCATCTTAATGATCC‑‑‑‑‑‑‑‑‑‑‑‑GTATTTCGGCTTCCTGAAACAACTGGCGCACGGTGATGTCGGTGCATCGTTTAAATATAAAGAGTATGCTATCAATGATTTGGTTGCGTCCGGTTTTCCCGTTTCGGCCAAACGTGGTGCCGCATCATTGTGCCTAGCTGTGATAGTTGGGGTTAGTACTGGCGTTa                                                                                  <  2:232792/251‑1 (MQ=255)                                                                                                                                          ggggcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgGATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAGGTGGGAGCCGCAGCATGTTTCCTTTCGGTAATACTGGGTGTTAGt                                                                                            <  2:179286‑M2/156‑1 (MQ=255)                                                                                                                                                 cgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCg                                                                                     <  1:104027‑M2/163‑1 (MQ=255)                                                                                                                                                      cgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTAtt                                                                                >  1:146857‑M2/84‑251 (MQ=255)                                                                                                                                                             cccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCAt                                                                         <  2:152256‑M2/175‑1 (MQ=255)                                                                                                                                                                                                                  cgaaatatcatcttaatgatccgTATTTCATCTACCTGAAACAACTGGCGCACGGTGCTTTCGGTCCATCGGTTGAATATAAAGCTTATTCGGTCAATGACTTGGATGCATCCAGTTTTCGCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCggg                    >  1:391239‑M2/24‑251 (MQ=255)                                                                                                                                                                                                                  cgaaatatcatcttaatgatccgTATTTCAGGTACCTGAAACAACTGACGCACGGTGGTTTCGGTCCATTGTTTAAATATAAAGATTATTCGTTCAATGACTTGTTTGCATCCAGTTTTTCCGTTTCTGCCATACTGAGAGCCGCAGCATTTTTCCTGGCGGTTATACTGGGTGTTAGTGCTTGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATTGTGTTGGCAATGATCggg                    >  1:231033‑M2/24‑251 (MQ=255)                                                                                                                                                                                                                  cgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGAGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCg                      >  1:70494‑M2/24‑249 (MQ=255)                                                                                                                                                                                                                           ggcttaatgatccgTATTTCAGCTAACTGAAACAACTGCCGCACGGTTATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCAGTTTCTGCCGAACTAGGAGCCGCAGCATTTTTCCTTGCGGTCATATTGGGTGTTAGTGATGGCGTTATTGCCGCATTAAAACAAGGCACCAAGTGGGACTTTACCTTGATTGGGCTGGCAATGAGcgggtttgtcatt           >  2:178225‑M2/15‑247 (MQ=255)                                                                                                                                                                                                                                    atccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGtttt  <  2:99530‑M2/246‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      CTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTT  >  NC_000913/1302853‑1303330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.