Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	987,152	G→T	intergenic (‑170/+433)	ompF ← / ← asnS	outer membrane porin F/asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	987,152	0	G	T	100.0%	73.6 / NA	21	intergenic (‑170/+433)	ompF/asnS	outer membrane porin F/asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/6);  total (15/6)

TGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAG  >  NC_000913/986914‑987396                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     tGCAGAGTTTGCAGCCCCTGCTACTACCAGAGCAGGGACGATCACTGACAGAATAATGAGCTTCATCATTATTTATTAAACTCATGGTTTTTTTTATGACAACTGCCACTGCCGTCAATCAGTTCTGTCAATACAAATTTACGGACCTATTGATCAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGttnaa                                                                                                                                                                                                                                           >  2:201723/1‑247 (MQ=255)  gCAGCGTTTGCAGTCCCTGCTACTAACAGCGCCGGGACGATCACTGTCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGTTTCaa                                                                                                                                                                                                                                          >  1:54886/1‑250 (MQ=255)   cAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGATTCATCATTATTTATTACCCTCATGGTTTTTTTTATGGCACCTGCCAATGCCGTCAATAAGTTTTGTCAAAAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTCTGTGTCTGCAGGCATCTTTCCCATCAAACTAACGTTTCGCTACCGTGAAAGTGCTTCAAAGATAAATATTTGGTTTCaaa                                                                                                                                                                                                                                         >  1:89447/1‑250 (MQ=255)      cGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGCATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGTTTCaaaa                                                                                                                                                                                                                                        >  2:134361/1‑248 (MQ=255)                    gCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGTTTCAAAAAGAAAAAATATGTAAcc                                                                                                                                                                                                                       >  2:35052/1‑251 (MQ=255)                                                                catcatTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTcc                                                                                                                                                                            <  1:7111/250‑1 (MQ=255)                                                                catcatTATATATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGTCCCTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTcc                                                                                                                                                                            >  2:32351/1‑250 (MQ=255)                                                                    attattTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTcaat                                                                                                                                                                          >  1:140895/1‑245 (MQ=255)                                                                               cccTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTgatgat                                                                                                                                                            >  2:59589/1‑251 (MQ=255)                                                                                                    catcTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTAATGATATTTACAGCAATTTTAATCTa                                                                                                                                       <  2:156170/248‑1 (MQ=255)                                                                                                        tGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGCTGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTAttt                                                                                                                                    >  1:211887/1‑250 (MQ=255)                                                                                                                                         aTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGAATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACgg                                                                                                  >  2:155222/1‑251 (MQ=255)                                                                                                                                            tACGGACCGATTGATGAGAGTTTGGTGTCCTTATGTGTCGGCAGGCATCTTACCAATCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGTTACAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGTCGTGATGATATTTTCAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAATCGGaa                                                                                                <  2:178626/250‑1 (MQ=255)                                                                                                                                             aCGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGAAAt                                                                                              <  1:75230/251‑1 (MQ=255)                                                                                                                                                                            aTGTGACTGCAGGCATCTTTCCATTACAACTAACGTTTCGCTACCGTGAAAGGGCTACAAAGATAAATATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATACAGACGTGATGATATTTACCGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTgg                                                               >  2:97287/1‑251 (MQ=255)                                                                                                                                                                                                  aTTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACAt                                         >  1:209290/1‑251 (MQ=255)                                                                                                                                                                                                       aaCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAATATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGAAATTTTTGTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGa                                    <  1:35052/251‑1 (MQ=255)                                                                                                                                                                                                                               gTGCTACAAAGATAAATATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGatat                                                                                                                                                          >  1:194203/1‑109 (MQ=255)                                                                                                                                                                                                                               gTGCTACAAAGATAAATATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGatat                                                                                                                                                          <  2:194203/109‑1 (MQ=255)                                                                                                                                                                                                                                    aCAAAGATAACTATTTGGTTTCATAACGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGAATTTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGACATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGCCATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCt       >  2:145562/1‑251 (MQ=255)                                                                                                                                                                                                                                    aCAAAGATAAATATTTGGTTTCAAAAAGACAAACTATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCt       >  1:67407/1‑251 (MQ=255)                                                                                                                                                                                                                                         gataaatatTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAg  <  2:54886/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     TGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAATTTTAATCTATTTATATGATTTCCTTATATTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAG  >  NC_000913/986914‑987396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑