Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,339,943 C→G G41G (GGG→GGC)  mlaF ← intermembrane phospholipid transport system, ATP binding subunit MlaF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,339,9430CG94.4% 49.5 / ‑5.9 18G41G (GGG→GGC) mlaFintermembrane phospholipid transport system, ATP binding subunit MlaF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (7/10);  minor base A (0/1);  total (7/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

ACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAA  >  NC_000913/3339705‑3340163
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                            
acaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGATCGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGcc                                                                                                                                                                                                                  <  1:26141/251‑1 (MQ=255)
     ggcccgGGAAGTTGGGTATGTTCGGGCAGTGGACAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGCTTTAAATAACATGCTCATCCTTTTGCGCACTGTATCCAGGCGAGAACGGGACATCGCCGGACTATTCTCACCATGGAAAAGGAGCTCCCCATGCTCTTGTGCCATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTTCCGATGCCCGATGGGCCCATGATCGCCGTGAt                                                                                                                                                                                                             <  1:95041/247‑1 (MQ=255)
                          tCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCa                                                                                                                                                                                         <  1:216136/250‑1 (MQ=255)
                                 gtgaaTAGGGGACGTTGTCAAATACGTTCATATCAGTAAACACCCCCCCAGACGGAAATAACATGCTCATCCGTTGGCGCACTGTATAAAGGCGCGAACGAGACATCGCCGGAATATTCTCCCCATCGAAAAGGATCTAACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAata                                                                                                                                                                                 <  2:195031/247‑1 (MQ=255)
                                                 gTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGACCGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGCTGGGCCCATGATCGCCGTGATCTTCCCTcgcg                                                                                                                                                                                                  >  2:183478/1‑218 (MQ=255)
                                                 gTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCcgcgcg                                                                                                                                                                                                  <  1:183478/218‑1 (MQ=255)
                                                             cATAGCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCCCTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCAcgcg                                                                                                                                                     <  1:113263/251‑1 (MQ=255)
                                                               aatCAGTGAACAACGCCCCGGACGAAAATAACAGGCGCCTCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCACACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTa                                                                                                                                                   <  2:103966/250‑1 (MQ=255)
                                                                    gTGAACAACGCCCCGGTCTCAAAAACCGTGCTCATCCTTATGCGCAATTTATACAGGCGCGAACGAGAGATCGCGGGAATATTCTCGCAATCGAAAAGGATCTCCCCGTAGTCTGGGGCGATTTGCCTGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCTCTCGCGGCACGTTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACt                                                                                                                                              <  2:40802/251‑1 (MQ=255)
                                                                                            aaCATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACCTCTCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGCAGCGTCGTTTTACCGATGCCCGCTGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAAtt                                                                                                                      >  1:150862/1‑251 (MQ=255)
                                                                                                                              gcgAACGAGACCTCACCGGAATATTCTCACCATCGAAGAGGATCTCACCATGCTCTGGTGCGATTTGCCCGCCAATCAGACGGCGTAGCGCCGTTTTACCGATGCTCGATGGGCCCATGATCGCCGTCATCTTCCCTCGCGGCACGGTCAGGGACATATCACCGAAGATGCCGCGCTTGCCACGCGTACAACTGACATAGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCt                                                                                    >  2:92913/1‑251 (MQ=255)
                                                                                                                               cgAACTAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCtt                                                                                   <  1:129189/251‑1 (MQ=255)
                                                                                                                                                      tctcaaaaTCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGACGTCATCTTCCCTCGCGGCACGGACAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGgcg                                                            >  1:141393/8‑251 (MQ=255)
                                                                                                                                                         cACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTc                                                         >  1:125193/1‑251 (MQ=255)
                                                                                                                                                                               acgATCTGATGCGAATTTACCGCGAATCAGGCGGAGTAGCGTCGGTTTACCGAAGCATTATGGAACCATGATCGCCGTGAGCTTCCCACGCGGCACGGTCAGTGAAATATTATCGAAGATGCAAGGATTGCCACGCTTAAAACTGACATTGCGCTTATCGACTAAATTCGCCACAGACTACTCCATAATACACCCTTCGTCTTGCGTTGATTTTCTAAGCATGTCGCTCTATGTACCCTTGAACCCTACat                                   <  2:63612/249‑1 (MQ=255)
                                                                                                                                                                                           gATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTaa                                                                    >  2:153263/1‑206 (MQ=255)
                                                                                                                                                                                           gATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTaa                                                                    <  1:153263/206‑1 (MQ=255)
                                                                                                                                                                                                                 aGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGaa  >  2:170587/1‑250 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                            
ACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAA  >  NC_000913/3339705‑3340163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑